Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics and breeding of a new cultivar Pleurotus eryngii var. ferulae, 'Beesan No.2'

아위느타리 신품종 '비산2호'의 육성 및 자실체 특성

  • Shin, Pyung-Gyun (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoo, Young-Bok (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2014.03.31
  • Accepted : 2014.04.03
  • Published : 2014.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

To develop a new cultivar of King oyster mushroom white variety (Pleurotus ferulae), GW10-68 as parental strain was selected by the method of Di-mon crossing between monokaryotic strain ASI 2798-24 derived from ASI 2798 and dikaryotic strain ASI 2803. The GW10-95(ASI 2803 ${\times}$ ASI 2798-24) was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Beesan No.2'. The 'Beesan No.2' was formed incompatibility line distinctly in the confrontation growth of parental strains ASI 2803, ASI 2798 and Beesan No.1. Analysis of the genetic characteristics of the new cultivar 'Beesan No.2' showed a different DNA profile as that of the control strains, ASI 2803, ASI 2798 and 'Beesan No.1', when RAPD(Random Amplified Polymorphic DNA) primer URP4 was used. The optimum temperature and pH arrange for mycelial growth were $25^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about 123.3 g. And also the stipe was long. This new cultivar 'Beesan No.2' of Pleurotus ferulae was characterized white-like variety of King oyster mushroom in the color of stipe, that was long and low yield compared to that of other cultuvar 'Beesan No.1'. We therefore expect that this new strain will increase of export and substitute cultivar of King oyster mushroom.

아위느타리(Pleurotus ferulae)의 품종 육성은 수량성이 높은 ASI 2803과 형태적으로 보기 좋은 외국 도입품종 ASI 2798의 단포자를 분리한 후 교배하여 13개의 교잡주를 우량계통으로 선발하였고, 수량성이 높은 ASI 2803보다 수량은 적으나 백색을 띄며 형태가 좋은 우량계통 GW10-68를 선발하여 농작물직무육성 신품종선정심의회에 상정하여 통과되어 '비산2호'로 명명되었다. 고유특성으로는 균사체 배양의 대선형성유무에서 모균주 ASI 2803, ASI 2798 및 비산1호와 대치배양하였을 때 뚜렸한 대선을 형성하였다. 유전적으로는 RAPD primer를 이용하여 신품종 '비산2호'의 DNA pattern는 ASI 2798 및 비산1호와는 DNA밴드 패턴이 구별되는 밴드양상을 보였으나 모균주 ASI 2803과는 유사한 밴드 패턴을 보여주었다. 가변특성은 균사생장 적온은 $25^{\circ}C$이며, pH의 범위가 pH5~8까지 넓게 형성되었다. 자실체 수량은 병당 $123.3{\pm}25.7g$으로 비산1호보다 수량이 적지만, 개체중에서는 오히려 높게 나타났다. 또한 대 길이는 비산1호보다 길고, 대의 색깔이 큰느타리보다 순백색을 나타내어 큰느타리와 차별화하여 재배하면 큰느타리 대체품종 뿐만 아니라 수출 품종으로도 기대된다.

Keywords

References

  1. Alam N, Shim MJ, Lee MW, Shin PG, Yoo YB, Lee TS. 2009. Phylogenetic relationship in different commercial strains of Pleurotus nebrodensis based on ITS sequence and RAPD. Mycobiology. 37(3) : 183-188. https://doi.org/10.4489/MYCO.2009.37.3.183
  2. Baldrian P, Gabriel J, Pospisck M. 1999. Improved isolated of nucleic acids from basidiomycetes fungi. Biotechniques. 27 : 458-460.
  3. Hong KH, Kim BY, Kim HK. 2004a. Analysis of nutritional components in Pleurotus ferulae. Korean J Food SCI Technol. 34(4) : 543-567.
  4. Hong KH, Kim BY, Kim HK. 2004b. Studies on the biological activity of Pleurotus ferulae. J Korean Soc Food Sci Nutr. 33(5) : 791-796. https://doi.org/10.3746/jkfn.2004.33.5.791
  5. Huang NL. 1996. Pleurotus eryngii var. nebrodensis. In: Huang NL (ed) Cultivation of 18 species of rare and delicious mushroom. Chinese Agriculture Press, Beijing, pp 17-21
  6. Kawai G, Babasaki K, Neda H. 2008. Taxonomic position of Chinese Pleurotus "Bai-ling-Gu": it belongs to Pleurotus eryngii (DC.: Fr) Quel. And evolved independently in China. Mycoscience. 49 : 75-87. https://doi.org/10.1007/S10267-007-0392-2
  7. Kim DS. 2002. Physiological characteristics of Pleurotus ferulea Lanzi. thesis for Ph.D. Chonbuk Univ.
  8. Kim KH, Yoo YB. 2007. Breeding of the cultivar of Pleurotus eryngii var. ferulae and their construction of stabilized production system. The Republic of Korea Ministry of Agriculture, Food and Rural Affairs. pp 96.
  9. Korea Seed & Variety Service. 2013. Beesan No.1. Patent 2013- 203(2013.3.8.)
  10. Korea Seed & Variety Service. 2014. Beesan No.2. Patent 2014- 68(2014.2.4.)
  11. Korea Seed & Variety Service. 2013. Mihyang. Name Patent 2007- 1144(2007.10.25.)
  12. Kwon KY, Cheong UJ. 2011. Mushmaru. Patent 10-2009- 0113872(2009.11.24.)
  13. Lee DH, Koo CD, Who B C. Kang BG, Choi JS. 2009. Mycelial Culture Characteristics of Pleurotus ferulae Strains. J Mushroom Sci Prod. 7(1) : 27-36.
  14. Lewinsohn D, Wasser SP, Reshetnikov SV, Hadar Y, Nevo E. 2002. The Pleurotus eryngii species-complex in Israel: distribution and morphological description of a new taxon. Mycotaxon. 81 : 51-67.
  15. Mao XL. 2001. Agaricales. In: Mao XL (ed) The macrofungi in China. Henan Science and Technology Press, Zhengzhou, pp 64-66.
  16. Mu CJ, Cao YQ, Ma JY. 1987. A new variety of Pleurotus ferula and its culture characters. Acta Mycol Sin 6 : 153-156
  17. Shin PG, Yoo YB, Kong WS, Oh YL. 2014. Characteristics and breeding of a new cultivar Pleurotus eryngii var. ferulae, Beesan No.1. J Mushrooms. 12(1):In press.
  18. Shin PG, Yoo YB, Kong WS, You CH, Oh SJ. 2004. Characterization of intraspecific hybrids by Di-mon crossing in Pleurotus eryngii. J Mushroom Sci Prod. 2(2) : 109-113.
  19. The Republic of Korea Ministry of Agriculture, Food and Rural Affairs, Korea Seed & Variety Service. 2012. Oyster Mushroom (Pleurotus (Fr.) Quel.), http://www.seed.go.kr.
  20. Venturella G, Zervakis G, Rocca SL. 2000. Pleurotus eryngii var. elaeoselini var. nov. from Sicily. Mycotaxon. 76 : 419-42.
  21. Venturella G. 2000. Typification of Pleurotus nebrodensis. Mycotaxon. 75 : 229-231.
  22. Zhang JX, Huang CY, Ng TB, Wang HX. 2006. Genetic polymorphism of ferula mushroom growing on Ferula sinkiangensis. Appl Microbiol Biotechnol. 71 : 304-309. https://doi.org/10.1007/s00253-005-0139-y