Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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SIFamide and SIFamide Receptor Define a Novel Neuropeptide Signaling to Promote Sleep in Drosophila

  • Park, Sangjin (Department of Biological Sciences, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology) ;
  • Sonn, Jun Young (Department of Biological Sciences, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology) ;
  • Oh, Yangkyun (Department of Biological Sciences, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology) ;
  • Lim, Chunghun (Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology) ;
  • Choe, Joonho (Department of Biological Sciences, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology)
  • Received : 2013.12.16
  • Accepted : 2014.03.04
  • Published : 2014.04.30
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Abstract

SIFamide receptor (SIFR) is a Drosophila G protein-coupled receptor for the neuropeptide SIFamide (SIFa). Although the sequence and spatial expression of SIFa are evolutionarily conserved among insect species, the physiological function of SIFa/SIFR signaling remains elusive. Here, we provide genetic evidence that SIFa and SIFR promote sleep in Drosophila. Either genetic ablation of SIFa-expressing neurons in the pars intercerebralis (PI) or pan-neuronal depletion of SIFa expression shortened baseline sleep and reduced sleep-bout length, suggesting that it caused sleep fragmentation. Consistently, RNA interference-mediated knockdown of SIFR expression caused short sleep phenotypes as observed in SIFa-ablated or depleted flies. Using a panel of neuron-specific Gal4 drivers, we further mapped SIFR effects to subsets of PI neurons. Taken together, these results reveal a novel physiological role of the neuropeptide SIFa/SIFR pathway to regulate sleep through sleep-promoting neural circuits in the PI of adult fly brains.

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References

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