Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Cinobufacin Suppresses Cell Proliferation via miR-494 in BGC-823 Gastric Cancer Cells

  • Zhou, Rong-Ping (First College of Clinical Medicine, Nanjing University of Chinese Medicine) ;
  • Chen, Gang (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University) ;
  • Shen, Zhi-Li (Department of Oncology, the Affiliated Jiangning Hospital of Nanjing Medical University) ;
  • Pan, Li-Qun (First College of Clinical Medicine, Nanjing University of Chinese Medicine)
  • Published : 2014.02.01
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Abstract

Cinobufacin is used clinically to treat patients with many solid malignant tumors. However, the mechanisms underlying action remain to be detailed. Our study focused on miRNAs involved in cinobufacin inhibition of GC cell proliferation. miRNA microarray analysis and real time PCR identified miR-494 as a significant cinobufacin-associated miRNA. In vivo, ectopic expression of miR-494 inhibited the proliferation and induced apoptosis of BGC-823 cells on CCK-8 and flow cytometry analysis. Further study verified BAG-1 (anti-apoptosis gene) to bea target of miR-494 by luciferase reporter assay and Western blotting. In summary, our study demonstrated that cinobufacin may inhibit the proliferation and promote the apoptosis of BGC-823 cells. Cinobufacin-associated miR-494 may indirectly be involved in cell proliferation and apoptosis by targeting BAG-1, pointing to use as a potential molecular target of cinobufacin in gastric cancer therapy.

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References

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