The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Yeasts Associated with Fruits and Blossoms Collected from Hanbat Arboretum, Daejeon, Korea

한밭 수목원의 과일과 꽃으로부터 효모의 분리

  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Min, Jin-Hong (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Seon-A (Hanbat Arboretum) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 민진홍 (배재대학교 바이오.의생명공학과) ;
  • 김선아 (한밭수목원) ;
  • 이종수 (배재대학교 바이오.의생명공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과)
  • Received : 2014.06.23
  • Accepted : 2014.06.26
  • Published : 2014.06.30
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Abstract

Yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various fruits and blossoms are limited. We collected different kinds of blossoms and unripened fruits from Hanbat arboretum, Daejeon, Korea at the year of 2013. Yeasts were isolated by plating of suspensions prepared for collected samples onto YPD medium containing antibiotics. BLAST searches were subsequently performed for the comparison of the partially determined sequences of D1/D2 domain of 26S rDNA. As a result, we isolated 57 yeast strains of 31 species from 29 different kinds of blossoms and 6 kinds of fruits samples. We found huge differences in yeast flora depending on the sample collection season.

효모는 엽권의 중요한 서식자이지만, 효모의 분포와 역할을 정확하게 이해하고 있지 못한 형편이다. 2013년 봄과 가을에, 대전시 서구 만년동에 위치한 한밭 수목원에 식재되어 있는 각종 꽃과 과일로부터 효모를 분리하였다. 분리된 효모 집락을 PCR 방법에 의해 26S rDNA의 D1/D2 지역을 증폭하고 염기서열을 결정한 후, BLAST를 이용하여 데이터베이스와 비교하여 효모를 동정하였다. 그 결과 29종의 꽃과 6종의 과일로부터, 14속 31종에 속하는 효모를 57균주를 분리하였다. 그 결과 봄과 가을, 계절에 따라서 같은 수목원에서 분리되는 효모 종에 현저한 차이가 있는 것을 발견하였다.

Keywords

References

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