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Predation Efficiency and Preference of the Hydrophilid Water Beetle Hydrochara affinis (Coleoptera: Hydrophilidae) Larvae on Two Mosquitos Culex pipiens molestus and Ochlerotatus togoi under Laboratory Conditions

잔물땡땡이(Hydrochara affinis: Hydrophilidae, Coleoptera) 유충의 모기 2종(Culex pipiens molestus 및 Ochlerotatus togoi)에 대한 실내조건에서의 섭식효율 및 선호도

  • Baek, Hak Myeong (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Dong Gun (Department of Life Science, Graduate School, Korea University) ;
  • Baek, Min Jeong (Department of Life Science, Graduate School, Korea University) ;
  • Lee, Cha Young (Department of Life Science, Graduate School, Korea University) ;
  • Kang, Hyo Jeong (Department of Life Science, Graduate School, Korea University) ;
  • Kim, Myeong Chul (SOKN Co., Ltd.) ;
  • Yoo, Jae Seung (ETND Co., Ltd.) ;
  • Bae, Yeon Jae (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University)
  • 백학명 (고려대학교 생명과학대학 환경생태공학부) ;
  • 김동건 (고려대학교 대학원 생명과학과) ;
  • 백민정 (고려대학교 대학원 생명과학과) ;
  • 이차영 (고려대학교 대학원 생명과학과) ;
  • 강효정 (고려대학교 대학원 생명과학과) ;
  • 김명철 ((주)SOKN 생태보전연구소) ;
  • 유재승 ((주)이-티엔디) ;
  • 배연재 (고려대학교 생명과학대학 환경생태공학부)
  • Received : 2014.03.10
  • Accepted : 2014.05.17
  • Published : 2014.06.30

Abstract

Predation efficiency and preference of the hydrophilid water beetle, Hydrochara affinis (Coleoptera: Hydrophilidae), larvae on two mosquito species, Culex pipiens molestus and Ochlerotatus togoi, were tested under laboratory conditions. Experiments were conducted in a transparent cylindrical plastic container (diameter, 100 mm; height, 40 mm; water level, 15 mm) for 24 hours (16 h, light; 8 h, dark) at $25^{\circ}C$. The predation efficiency of H. affinis on mosquito larvae was investigated under various prey conditions (1, 2, 4, 8, 16, 32, 64, and 128 larvae) with five replicates. Further, in order to investigate the prey preference on two mosquito species, each 32 larvae of C. pipiens molestus and O. togoi were provided simultaneously for the first instar larva of H. affinis with 5 replicates. As a result, the predation curves of H. affinis on two mosquito species were logarithmic; and the number of consumed prey increased as the number of predator instars were increased. As predicted from the predation curves, the estimated maximum number of C. pipiens molestus and O. togoi consumed by a third instar larva of H. affinis per a day was 926 and 304 larvae, respectively. As predicted from the Holling's disk equation, the differences in consumed prey number between predator instars and prey species were caused by handling time rather than attack rate. Handling time decreased rapidly as the predator larvae grew, and that of O. togoi was twice longer than that of C. pipiens molestus.

잔물땡땡이(Hydrochara affinis) 유충의 지하집모기(Culex pipiens molestus)와 토고숲모기 (Ochlerotatus togoi)에 대한 포식효율과 먹이선호도를 실험하였다. 포식실험에는 투명한 원통형 용기(지름 100 mm, 높이 40 mm, 수심 15 mm)가 사용되었으며, $25^{\circ}C$에서 24시간 (16L : 8D)동안 진행되었다. 잔물땡땡이 개체당 모기 섭식 효율을 조사하기 위해 각 잔물땡땡이 유충에 대해 서로 다른 먹이조건(1, 2, 4, 8, 16, 32, 64 및 128개체)을 주었고, 각 조건별로 5회씩 반복실험 하였다. 모기유충 2종에 대한 먹이 선호도를 조사하기 위해 잔물땡땡이 1령 유충에 지하집모기와 토고숲모기 유충 32개체씩을 동시에 투여하여 5회 반복실험 하였다. 실험의 결과 모기유충 2종에 대한 잔물땡땡이 유충의 포식곡선은 로그함수의 곡선으로 나타났으며, 잔물땡땡이 유충의 영기가 증가함에 따라 포식수도 증가하는 것으로 나타났다. 포식곡선으로부터 잔물땡땡이 3령 유충의 1일 최대 포식수를 산출한 결과, 지하집모기는 926개체를, 토고숲모기는 304개체를 포식 할 것으로 추정되었다. Holling's disk equation을 이용하여 추정한 결과, 잔물땡땡이 유충의 영간, 그리고 모기유충의 종간에 모기유충 포식수의 차이가 발생하는 것은 Attack rate보다는 Handling time의 차이에 기인한 것으로 사료된다. Handling time은 잔물땡땡이 유충이 성장함에 따라 급격히 감소하였으며, 토고숲모기가 지하집모기에 비해 2배가량 길게 나타났다.

Keywords

References

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