Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Study to find the Optimal Purification Processing Conditions of Anthocyanin from Bokbunja Byproducts

복분자박을 이용한 안토시아닌 최적정제공정 연구

  • Received : 2013.11.28
  • Accepted : 2014.01.15
  • Published : 2014.02.28
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Abstract

This study was performed to determine the purification yield, color value, and total anthocyanin content of purified anthocyanins from Bokbunja byproducts for analyzing the optimal conditions of purification processing by response surface methodology (RSM) based on HCl or trifluoroacetic acid (TFA) concentration as an acidulent and ethanol concentration as an eluent. From the results of purification yield, color value, and total anthocyanin content of the purified anthocyanins, it was shown that HCl was better than TFA for purification processing from Bokbunja byproducts and the optimal concentration range of ethanol was 60-75%. The optimal purification conditions for HCl or TFA, ethanol concentration, and overall desirability by analysis of multiple response surface methodology with the same weighed value for each dependent variable were 0.84%, 73.12% and 0.77 for HCl acidulent and 1.00%, 60.75%, and 0.96 for TFA acidulent, respectively.

본 연구는 복분자박의 안토시아닌을 천연색소원으로 활용하기 위하여 MCX SPE cartridge를 흡착제로 사용하였으며 pH 조절제로서 HCl 또는 trifluoroacetic acid(TFA)의 농도 변화 및 용출제로서 ethanol 농도 변화에 따른 수율, 색 가 및 총 안토시아닌 함량 등을 반응표면분석법에 의하여 최적화하였다. 정제된 안토시아닌의 수율, 색가 및 총 안토시아닌 함량을 전반적으로 분석한 결과 pH 조절제로서 HCl이 TCA보다 우수하게 나타났으며 용출제로서의 ethanol의 농도로는 60-75%수준에서 가장 좋은 결과를 얻었다. 다중반응표면의 최적화방법으로써 만족함수(desirability function)를 이용하여 종속변수들의 가중치를 1로 놓고 분석한 결과 pH 조절제가 HCl인 경우 최적 분리정제 조건은 HCl 0.84%, ethanol 73.12%의 조건이었으며 전반적인 만족도는 0.77이었다. TFA에서의 최적 분리정제조건은 TFA 1.0%, ethanol 60.75%의 조건이었으며 전반적인 만족도는 0.96이었다.

Keywords

Acknowledgement

Supported by : 농림수산식품기술기획평가원

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