Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Analysis of Fatty Acid Composition and Effects of Pumpkin Seed Oil on Human Umbilical Vein Endothelial Cells

호박씨유의 지방산 성분 분석 및 Human Umbilical Vein Endothelial Cell에 미치는 영향 연구

  • Kim, Kyoung Kon (Dept. of Bio-Health Technology, Kangwon National University) ;
  • Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University) ;
  • Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University) ;
  • Lee, Jeong Il (Basic Lab., Hanbit Flavor & Frganace) ;
  • Choe, Myeon (Dept. of Bio-Health Technology, Kangwon National University)
  • 김경곤 (강원대학교 생명건강공학과) ;
  • 강윤환 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김대중 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 김태우 (강원대학교 강원웰빙특산물산업화지역혁신센터) ;
  • 이정일 ((주)한빛향료) ;
  • 최면 (강원대학교 생명건강공학과)
  • Received : 2014.03.26
  • Accepted : 2014.06.28
  • Published : 2014.06.30
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Abstract

Pumpkin seed oil (PSO) was investigated for its parasite elimination activity and efficacy in treating disorders of the prostate gland and urinary bladder. We confirmed the composition of PSO and identified its ability to improve vessels. Gas chromatography coupled with mass spectrometric (GC-MS) system was used for PSO composition analysis. Cytotoxicity and cell proliferation were confirmed by Cell Counting Kit-8 (CCK-8) assay. Nitric oxide(NO) production was measured with Griess reagent, and intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 mRNA expression levels were measured by reverse transcription polymerase chain reaction (RT-PCR). As a result, PSO revealed the presence of several components such as linoleic acid, oleic acid, palmitic acid and stearic acid. Cytotoxic effects of PSO were not observed, and PSO increased nitric oxide production in human umbilical vein endothelial cells (HUVEC). Additionally, TNF-${\alpha}$-induced cell proliferation and ICAM-1 expression in HUVEC were inhibited by PSO treatment, whereas VCAM-1 expression was not significantly reduced. Taken together, these results show that PSO is worthy of study as a candidate food material for improvement of vascular disease.

본 연구에서는 호박씨유의 성분 특성과 기능성의 기초 자료 확보를 위해 수행되었다 그 결과, 호박씨유는 알려진 바와 같이 linoleic acid(44.7%), oleic acid(25.3%), palmitic acid(17.4%), stearic acid(7.9%)가 분석되었으며, 미량의 arachidonic acid(0.4%) 또한 함유하고 있었다. 세포 독성 실험을 통해 0.2 mg/mL 농도까지 독성이 관찰되지 않았고, 그 이상의 농도에서도 호박씨유보다는 용매에 의한 독성이 관찰되었다. 지방 성분 분석을 통해 구성 성분 및 함유량이 확인된 호박씨유를 이용하여 혈관 보호 및 질병 예방에 대한 잠재적 기능성을 연구하기 위해 nitric oxide 분비량 측정, 대표적인 세포 부착 단백질인 ICAM-1 및 VCAM-1의 발현과 cell proliferation 측정한 결과, 호박씨유는 TNF-${\alpha}$에 의해 감소된 nitric oxide를 유의하게 증가시켰다. 또한 ICAM-1과 VCAM-1의 발현을 확인한 결과, ICAM-1은 유의한 수준으로 감소되었고, 반면 VCAM-1은 감소하는 경향은 보였으나, 통계적인 유의성은 관찰되지 않았다. 호박씨유의 HUVEC proliferation 억제 효과는 TNF-${\alpha}$ 100 ng/mL 처리군(113%)과 비교하여 PSO 0.01 mg/mL, 0.05 mg/mL 및 0.1 mg/mL를 처리 군에서 100.7%, 100.8%, 90.3%의 억제 효과가 관찰되어 무처리 control군과 유사한 수준을 유지하는 것으로 관찰되었다. 위의 연구 결과를 종합해 보면, 호박씨유는 불포화지방산이 다량 함유된 우수한 식물성 유지이며, 기능적으로 혈관 보호 및 질병 예방에 잠재적으로 우수한 활성이 있음을 확인할 수 있었다. 따라서 본 연구 결과를 기초 자료로 하여 효과적인 기능성 식품 및 소재의 개발이 가능할 것으로 판단된다.

Keywords

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