Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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A Long Non-Coding RNA snaR Contributes to 5-Fluorouracil Resistance in Human Colon Cancer Cells

  • Lee, Heejin (Department of Biochemistry, College of Medicine, Catholic University of Korea) ;
  • Kim, Chongtae (Department of Biochemistry, College of Medicine, Catholic University of Korea) ;
  • Ku, Ja-Lok (Cancer Research Institute and Cancer Research Center, Seoul National University) ;
  • Kim, Wook (Department of Molecular Science and Technology, Ajou University) ;
  • Kim Yoon, Sungjoo (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea) ;
  • Kuh, Hyo-Jeong (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea) ;
  • Lee, Jeong-Hwa (Department of Biochemistry, College of Medicine, Catholic University of Korea) ;
  • Nam, Suk Woo (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea) ;
  • Lee, Eun Kyung (Department of Biochemistry, College of Medicine, Catholic University of Korea)
  • Received : 2014.06.05
  • Accepted : 2014.06.30
  • Published : 2014.07.31
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Abstract

Several types of genetic and epigenetic regulation have been implicated in the development of drug resistance, one significant challenge for cancer therapy. Although changes in the expression of non-coding RNA are also responsible for drug resistance, the specific identities and roles of them remain to be elucidated. Long non-coding RNAs (lncRNAs) are a type of ncRNA (> 200 nt) that influence the regulation of gene expression in various ways. In this study, we aimed to identify differentially expressed lncRNAs in 5-fluorouracil-resistant colon cancer cells. Using two pairs of 5-FU-resistant cells derived from the human colon cancer cell lines SNU-C4 and SNU-C5, we analyzed the expression of 90 lncRNAs by qPCR-based profiling and found that 19 and 23 lncRNAs were differentially expressed in SNU-C4R and SNU-C5R cells, respectively. We confirmed that snaR and BACE1AS were down-regulated in resistant cells. To further investigate the effects of snaR on cell growth, cell viability and cell cycle were analyzed after transfection of siRNAs targeting snaR. Down-regulation of snaR decreased cell death after 5-FU treatment, which indicates that snaR loss decreases in vitro sensitivity to 5-FU. Our results provide an important insight into the involvement of lncRNAs in 5-FU resistance in colon cancer cells.

Keywords

References

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