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Detection Characteristics of a Red Blood Cell Coupled with Micron Magnetic Beads by Using GMR-SV Device

GMR-SV 소자를 이용한 미크론 자성비드와 결합된 적혈구 검출 특성 연구

  • Lee, Jae-Yeon (Dept. of Oriental Biomedical Engineering, Sangji University) ;
  • Kim, Moon-Jong (Dept. of Oriental Biomedical Engineering, Sangji University) ;
  • Lee, Sang-Suk (Dept. of Oriental Biomedical Engineering, Sangji University) ;
  • Rhee, Jin-Kyu (Division of Analytical Research, Western Seoul Center, Korea Basic Science Institute)
  • 이재연 (상지대학교 한방의료공학과) ;
  • 김문종 (상지대학교 한방의료공학과) ;
  • 이상석 (상지대학교 한방의료공학과) ;
  • 이진규 (한국기초과학지원연구원 서울서부센터 분석연구부)
  • Received : 2014.06.17
  • Accepted : 2014.07.01
  • Published : 2014.08.31

Abstract

The glass/Ta(5.8 nm)/NiFe(5 nm)/Cu(2.3 nm)/NiFe(3 nm)/IrMn(12 nm)/Ta(5.8 nm) GMR-SV (giantmagneto-resistance-spin valve) multilayer structure films with a magnetoresistance ratio (MR) of 5.0 % and a magnetic sensitivity (MS) of 1.5%/Oe was deposited by dc magnetron sputtering method. Also, GMR-SV device having a width of $7{\mu}m{\sim}8{\mu}m$ similar to the diameter of RBC (red blood cell) was fabricated by the light lithography process. When RBCs coupled with several magnetic beads with a diameter of $1{\mu}m$ dropped upon the GMR-SV device having MR = 1.06% and MS = 0.3 %/Oe, there is observed the variation of about included of a resistance value of ${\Delta}R=0.4{\Omega}$ and ${\Delta}MR=0.15%$ around a external magnetic field of -0.6 Oe. From these results, the GMR-SV device having the width magnitude of a few micron size can be applied as the biosensor for the analysis of a new magnetic property of hemoglobin inside of RBC combined to magnetic beads.

Dc 마그네트론 스퍼터링법으로 자기저항비 5.0 %와 자장감응도 1.5 %/Oe를 갖는 glass/Ta(5.8 nm)/NiFe(5 nm)/Cu(2.3 nm)/NiFe(3 nm)/IrMn(12 nm)/Ta(5.8 nm) 다층구조 GMR-SV 박막을 증착하였다. 광 리소그래피 공정으로 적혈구 직경 크기인 $7{\mu}m{\sim}8{\mu}m$ 이내의 선폭인 GMR-SV 소자를 제작하였다. 직경 $1{\mu}m$ 크기인 여러 개의 자성비드가 결합된 적혈구를 자기저항비 1.06 % 자장감응도 0.3 %/Oe를 갖는 GMR-SV 소자에 떨어뜨려 -0.6 Oe 부근에서 약 $0.4{\Omega}$과 약 0.15 %의 변화를 관찰하였다. 본 연구로부터 미크론 크기의 선폭을 갖는 GMR-SV 소자가 자성비드를 결합한 적혈구내 헤모글로빈의 새로운 자성 특성을 분석하는 바이오센서로 활용할 수 있음을 보여주었다.

Keywords

References

  1. S. X. Wang and A. M. Taratorin, Magnetic Information Storage Technology, Academic Press (1999).
  2. D. A. Baker, Nature 405, 39 (2000). https://doi.org/10.1038/35011000
  3. R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger, and C. A. Mirkin, Science 277, 1078 (1997). https://doi.org/10.1126/science.277.5329.1078
  4. Y. Cui, Q. Wei, H. Park, and C. M. Lieber, Science 293, 1289 (2001). https://doi.org/10.1126/science.1062711
  5. S. H. Hong, Physics and High Technology 18, 16 (2009).
  6. D. L. Graham, H. A. Feliciano, P. P. Fretias, L. A. Clarke, and M. D. Amaral, Sens. Actuators B 107, 936 (2005). https://doi.org/10.1016/j.snb.2004.12.071
  7. B. M. de Boer, J. A. H. M. Kahlman, T. P. G. H. Jansen, H. Duric, and J. Veen, Biosens. Bioelectron. 22, 2366 (2006).
  8. J. Kong, N. R. Franklin, C. Zhou, Mi. G. Chapline, S. Peng, K. Cho, and H. Dai, Science 287, 622 (2000). https://doi.org/10.1126/science.287.5453.622
  9. S. S. Lee, S. H. Park, and K. S. Soh, Sae Mulli 52, 564 (2006).
  10. S. H. Park, K. S. Soh, M. C. Ahn, D. G. Hwang, and S. S. Lee, J. Korean Magn. Soc. 16, 157S (2006). https://doi.org/10.4283/JKMS.2006.16.3.157
  11. W. H. Lee, D. G. Hwang, and S. S. Lee, J. Magn. 14, 18 (2009). https://doi.org/10.4283/JMAG.2009.14.1.018
  12. J. G. Choi, I. S. Koh, Y. M. Gong, M. H. Kim, Y. S. Park, D. G. Hwang, and S. S. Lee, J. Korean Magn. Soc. 19, 52 (2009). https://doi.org/10.4283/JKMS.2009.19.2.052
  13. P. Khajidmaa and S. S. Lee, J. Korean Magn. Soc. 23, 193 (2013). https://doi.org/10.4283/JKMS.2013.23.6.193
  14. J. G. Choi, T. J. Kwak, J. T. Shim, and S. S. Lee, J. Korean Magn. Soc. 20, 35 (2010). https://doi.org/10.4283/JKMS.2010.20.2.035
  15. P. Khajidmaa, K. J. Park, and S. S. Lee, J. Korean Magn. Soc. 23, 98 (2013). https://doi.org/10.4283/JKMS.2013.23.3.098
  16. J. G. Choi and S. S. Lee, J. Korean Magn. Soc. 20, 41 (2010). https://doi.org/10.4283/JKMS.2010.20.2.041
  17. J. G. Choi, Y. S. Park, and S. S. Lee, J. Korean Magn. Soc. 22, 173 (2012). https://doi.org/10.4283/JKMS.2012.22.5.173

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