The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells

  • Ahn, Mi-Ja (Department of Pharmacognosy, Chung-Ang University College of Pharmacy) ;
  • Hur, Sun-Jung (Department of Pharmacognosy, Chung-Ang University College of Pharmacy) ;
  • Kim, Eun-Hyun (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Lee, Seung Hoon (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Shin, Jun Seob (Department of Biochemistry, Chung-Ang University College of Medicine) ;
  • Kim, Myo-Kyoung (Thomas J. Long School of Pharmacy, University of the Pacific) ;
  • Uchizono, James A. (Thomas J. Long School of Pharmacy, University of the Pacific) ;
  • Whang, Wan-Kyunn (Department of Pharmacognosy, Chung-Ang University College of Pharmacy) ;
  • Kim, Dong-Seok (Department of Biochemistry, Chung-Ang University College of Medicine)
  • Received : 2014.01.22
  • Accepted : 2014.05.19
  • Published : 2014.08.30
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Abstract

In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than $50{\mu}M$ and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.

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