Quality Characteristics of Citrus Fruit by Cyclic Low Pressure Drying and High Hydrostatic Pressure Extraction

초고압 처리에 의한 감귤의 추출률 및 특성변화

  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym Polytechnic University Research Institute of Biomaterial, Hallym Polytechnic University) ;
  • Choi, Young-Bum (Agro Foodtech Holdings O'JEJU, Jeju Special Self-Governing Province) ;
  • Ko, Jung-Rim (Agro Foodtech Holdings O'JEJU, Jeju Special Self-Governing Province) ;
  • Rha, Young-Ah (Dept. of Food Technology and Services, Eulji Univrsity) ;
  • Lee, Hyeon-Yong (Dept. of Food Science and Technology, Seowon University)
  • 박성진 (한림성심대학교 관광외식조리과/한림성심대학교 생물소재연구소) ;
  • 최영범 (농업회사법인 (주)오'제주) ;
  • 고정림 (농업회사법인 (주)오'제주) ;
  • 나영아 (을지대학교 식품산업외식학과) ;
  • 이현용 (서원대학교 식품공학과)
  • Received : 2014.02.05
  • Accepted : 2014.06.10
  • Published : 2014.06.30

Abstract

We developed a method for improving the antioxidant activities of Citrus extracts through cyclic low pressure drying(CLPD) and a high hydrostatic pressure extraction (HPE) process. Citrus fruits were prepared for water extraction at $60^{\circ}C$ and 300 MPa for 5 min (high hydrostatic pressure extraction, HPE5) and 15 min (high hydrostatic pressure extraction, HPE15) after cyclic low pressure drying method. Extraction yields obtained by cyclic low pressure drying and high hydrostatic pressure extraction process were 20.41, 23.47, and 28.19%, respectively. Total polyphenol contents were increased by combined process. Generally, CLPD and HPE resulted in higher yields than the conventional extraction process. Further, HPE15 showed 48.21% DPPH radical scavenging activity (EDA, %) at $1,000{\mu}g/mL$. In general, antioxidant activities of Citrus increased by CLPD and HPE. Therfore, CLPD and HPE of Citrus resulted in higher antioxidant activity than conventional water extraction.

본 연구에서는 순환형 감압건조 및 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 복합 추출공정에 의한 감귤의 항산화 활성 증진을 확인하고자 연구를 수행하였다. 건조공정을 거친 후 초고압 처리 추출물의 수율이 20.41~28.19%로 높은 추출 수율을 나타내어 건조전 열수추출공정(17.21%)과 비교하여 약 1.6배의 높은 추출수율을 나타내었다. 순환형 감압건조와 초고압 공정을 병행하였을 시 총 폴리페놀과 플라보노이드 함량이 순환형 감압건조공정을 거치지 않은 것보다 다소 증가되는 것으로 보아 활성성분의 용출이 증진된 것으로 보인다. DPPH radical 소거 활성은 15분 초고압 처리한 추출물이 48.21%로 높은 활성을 나타내었다. 전처리 공정에 따른 감귤 시료의 주사전자현미경(SEM)을 통해 순환형 감압건조 후 초고압 추출이 감귤 내부 조직까지 영향을 주어 세포벽이 깨어지면서 조직 및 구조가 변화한 것으로 판단되며, 이를 통해 수율 및 활성 성분의 용출 증가가 이루어 진 것으로 사료된다. 따라서, 감귤의 건조 및 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 추출수율을 향상시킬 것으로 판단된다.

Keywords

References

  1. Bennett PB, Marquis RE, Demchenko I (1998). High pressure biology and medicine. University of Rochester Press, New York, USA. p. 1-428
  2. Cheigh CI, Jung WG, Chung EY, Ko MJ, Cho SW, Lee JH, Chang PS, Park YS, Paik HD, Kim KT, Chung MS (2010). Comparison on the extraction efficiency and antioxidant activity of flavonoid from Citrus Peel by different extraction methods. Food Engineering Progress 14(2): 166-172.
  3. Choi KH, Jeong JS, Moon CH, Kim ML (2004). Effect of carbon source supplement on the gel production from citrus juice by Gluconacetobacer Hansenii TL-2C. J Korean Soc Food Sci Nutr 33(1): 170-175. https://doi.org/10.3746/jkfn.2004.33.1.170
  4. Choi YB (2010). The Manufacturing Method of Dried Kimchi by Using Reduced Pressure Drying. Korea Patent. 1020100077718
  5. Chung SK, Kim SH, Choi YH, Song EY, Kim SH (2000). Status of citrus fruit production and view of utilization in Cheju. Food Ind Nutr 5(2): 42-52
  6. Eun JB, Jung YM, Woo GJ (1996). Identification and determination of dietary fibers and flavonoids in pulp and peel of Korean tangerine (Citrus aurantium var.). Korean J Food Sci Technol 28(2): 371-377.
  7. George JP, Datta AK (2002). Development and validation of heat and mass transfer models for freeze-drying of vegetable slices. J Food Eng 52(1): 89-93. https://doi.org/10.1016/S0260-8774(01)00091-7
  8. Gutfinger T (1981). Polyphenols in olive oils. JAOCS 58(11): 966-967.
  9. He XL, Kim SS, Park SJ, Seong DH, Yoon WB, Lee HY, Park DS, Ahn JH (2010). Combined effectes of probiotic fermentation and highpressure extraction on the antioxidant, antimicrobial, and antimutagenic activities of Deodeok(Codonopsis lanceolata). J Agric Food Chem 58: 1719-1725. https://doi.org/10.1021/jf903493b
  10. Hwang OS, Park HJ, Chun HK, Chang CM (1990). A study on the manufacturing of vinegar from fallen apples. Res Rept RDA 32: 40-47.
  11. Kang SH, Lee YJ, Lee CH, Kim SJ, Lee DH, Lee YK, Park DB (2005). Physical activities of peel of Jeju-indifenous Citrus sunki Hort. Tanaka. Korean J Food Sci Technol 37(6): 983-988.
  12. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, Park UY, Lee HY (2008). Skin-Whitening and UV protective effects of Angelica gigas Nakai extracts on uitra high pressure extraction process. Korean J Medicinal Crop Sci 16(4): 255-260.
  13. Kim CH, Kwon MC, Syed AQ, Hwang B, Nam JH, Lee HY (2007). Toxicity reduction and improvement of anticancer activities from Rhodiola sachalinensis A. Bor by ultra high pressure extracts process. Korean J Medicinal Crop Sci 15(6): 411-416.
  14. Kim JS, Ahn JS, Ahn KY (2013). Quality characteristics of fresh noodles with hot-air-dried Perilla leaf powder. Korean J Culinary Research 19(3): 73-86.
  15. Lee EJ, Ju HW, Lee KS (2012). Quality characteristics of pan bread added with citrus mandarin peel powder. Korean J Culinary Research 18(1): 27-39.
  16. Ling J, Han JG, Ha JH, Jeong HS, Kwon MC, Ahn JH, Kim JC, Choi GP, Chung EK, Lee HY (2008). Effect of immune activity on Berberis koreana Palibin by ultra high pressure low temperature process. Korean J Medicinal Crop Sci 16(6): 439-445.
  17. Lee HH, Lee SY (2008). Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean J Medicinal Crop Sci 16(2): 79-85.
  18. Moon YG, Lee KJ, Heo MS (2007). Characteristics of citrus by-product ferment using Bacillus subtilis as starter extracts. Korean J Microbiol Biotechnol 35(2): 142-149.
  19. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY (2004). Effect of ultrasonification process on enhancement of immuno-stimulatory activity of Ephedra sinica Stapf and Rubus coreanus Miq. Korean J Biotechnol Bioeng 19(2): 113-117.
  20. Park SJ, Choi YB, Ko JR, Kim YE, Lee HY (2014). Enhancement of Antioxidant Activities of Blue berry (Vaccinium Ashei) by using High-Prerssure Extraction Process. J Korean Soc Food Sci Nutr 43(3): 471-476. https://doi.org/10.3746/jkfn.2014.43.3.471
  21. Que F, Mao L, Zhu C, Xie G (2006). Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. LWT-Food Sci Technol 39(2): 111-117. https://doi.org/10.1016/j.lwt.2005.01.001
  22. Rhee CO, Shin DH, Yoon IH, Han PJ (1979). Studies on the processing quality of Korean Citrus fruits. J Korean Soc Agric Chem Biotechnol 22(1): 28-32.
  23. Rhyu MR, Kim EY, Bae IY, Park YK (2002). Contents of naringin, hesperidin and neohesperidin in premature Korean citrus fruits. Korean J Food Sci Technol 34(1): 132-135.
  24. Youn KS (1998). Utilization of osmotic dehydration as pretreatment prior to drying. Korean J Postharvest Sci Technol 5(3): 305-314.