Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Studies on Insect Diversity Related to Drought-tolerant Transgenic (Agb0103) Rice in Large Scale GMO Field

대규모 GMO 포장에서 가뭄저항성벼(Agb0103)가 곤충다양성에 미치는 영향

  • Oh, Sung-Dug (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jinseo (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Kijong (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Tae-Hun (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Sang Jae (School of Applied Biosciences, Kyungpook National University)
  • 오성덕 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 김진서 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이기종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 류태훈 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 서상재 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2014.05.12
  • Accepted : 2014.06.09
  • Published : 2014.06.30
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Abstract

This study was carried out to develop of macro-protocol and the biosafety guide for drought-tolerant transgenic rice (Agb0103) at large scale GMO field, a total of $4,700m^2$. In GMO quarantine area of Kyungpook National University, insect species diversities and population densities on Agb0103 and wild type (Ilmi) were investigated. There was no difference between the population densities of insect pests and natural enemies on two varieties, while sometimes insect pest density on Ilmi was slightly higher than on Agb0103, but natural enemy density on Agb0103 was a little higher. These results provided the insect diversity for risk assessment analysis of Agb0103 and suggested that the macro-protocol could be useful to detect GM plants.

본 연구는 대규모 GMO 격리포장에서 고추 유래 가뭄저항성CaMsrB2유전자가 도입된 가뭄저항성벼(Agb0103)의 안정성 평가에 대한 가이드라인 및 프로토콜을 개발하고자 수행하였다. 경북대학교 농업생명과학대학 GMO 실습격리포장에서 총 사용면적 $4,700m^2$에서 Agb0103와 모품종인 일미벼에 대해 거미류를 포함한 곤충류 다양성을 조사하였다. 조사기간 동안 채집된 개체들은 기능별로 해충군, 천적군, 거미군으로 크게 구분하여 계수하였으며, 조사된 개체군의 해충군, 천적군, 거미류 등의 밀도는 기능군 별로 Agb0103와 일미벼간에 유의성을 보이지 않았다. 해충의 발생양상을 비교 분석한 결과, 전반적으로 일미벼에서 발생량이 다소 많은 경향으로 나타났으나, 대부분의 시기별 발생량 평균 간의 차이는 없는 것으로 나타나서 Agb0103와 일미벼과의 해충 발생양상에는 유의성을 찾기가 어렵다고 판단된다. 또한 천적류 발생양상은 해충 발생양상과는 달리 Agb0103에서 발생량이 다소 많은 경향으로 나타났으나, 시기별 평균 간 차이는 없는 것으로 분석되어 천적 발생양상에도 특이한 유의성을 없다고 판단된다. 따라서 가뭄저항성 CaMsrB2유전자가 도입된 Agb0103에서의 해충 및 천적의 개체군 밀도 및 발생양상은 모본인 일미벼에서와 차이가 없는 것으로 조사되었다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

References

  1. Brookes G, Barfoot P. 2006. Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use. AgBioForum 9: 139-151.
  2. Catling HD, Lee SC. 1977. Studies on economic damage of Korean rice pests. Kor. J. Pl. Prot. 16: 79-86.
  3. Cho SK, Ryu MY, Song C, Kwak JM, Kim WT. 2008. Arabidopsis PUB22 and PUB23 are homologous U-Box E3 ubiquitin ligases that play combinatory roles in response to drought stress. Plant Cell 20: 1899-1914. https://doi.org/10.1105/tpc.108.060699
  4. Dunwell JM. 1999. Transgenic crops: the next generation, or an example of 2020 vision. Ann. Bot. 84: 269-277. https://doi.org/10.1006/anbo.1999.0934
  5. Im JS, Cho KS, Cho JH, Park YE, Cheun CG, Kim HJ, Cho HM, Lee JN, Jin YI, Byun MO, Kim DY, Kim MJ 2012. Growth, quality, and yield characteristics of transgenic potato (Solanum tuberosum L.) overexpressing StMyb1R-1 under water deficit. J. Plant Biotech. 39: 154-162. https://doi.org/10.5010/JPB.2012.39.3.154
  6. James C. 2013. The global status of commercialized biotech/GM Crops: 2013. ISAAA Briefs No. 46: 4-8.
  7. Jang IC, Oh SJ, Seo JS, Choi WB, Song SI, Kim CH, Kim YS, Seo HS, Choi YD, Nahm BH, Kim JK. 2003. Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. Plant Physiol. 131: 516-24. https://doi.org/10.1104/pp.007237
  8. Kim CK, Min HS, Ra DS, Lee KS. 1996. Incidence of major rice diseases under direct seeded cultivation. RDA. J. Agri. Sci. 38: 353-358.
  9. Kim JS, Park HM, Che SH, Lee TH, Hwang DJ, Oh SD, Park JS, Song DG, Pan CH, Choi DI, Kim YH, Nahm BH, Kim YK. 2013. A Pepper MSRB2 gene confers drought tolerance in rice through the protection of chloroplast-targeted genes. Plos One. 9: e90588.
  10. Kim KM, Ryu TH, Suh SJ. 2010. Studies on insect diversity realted to genetically engineered vitamin A rice under large scale production. Korean J. Breed. Sci. 42: 157-162.
  11. Lee HP, Kim JP, Jun JR. 1993. Utilization of insect natural enemies and spiders for the biological control in rice paddy field - Community structure of insects and spiders, suppress effect on insect pest by natural enemies, and their overwintering habitats in rice paddy field. RDA. J. Agri. Sci. 35: 261-274.
  12. Lee HS, Ryu TH, Jung HY, Park SK, Park GH, Sohn JK, Kim KM. 2010. Characteristics of agronomy to vitamin A strengthening rice at large scale GMO field. Korean J. Breed. Sci. 42: 56-60.
  13. Lee SC, Ma KC. 1997. Occurrence of major insect pests in machine transplanted and direct seeded rice paddy field. Korean J. Entomol. 36: 141-144.
  14. Lee SG, Lee YH, Kim JS, Lee BM, Kim MJ, Shin JH, Kim HM, Choi DH. 2005. Insect pests occurrence and control in organic and conventional rice paddy field. Korean J. Organic Agri. 13: 301-201.
  15. Lee SW, Choi BR, Park HM, Yoo JK, Chung BK. 2005. Monitoring on insecticide resistance of major insect pests in paddy field. Korean J. Pesticide Sci. 9: 365-373.
  16. Ma KC, Lee SC. 1996. Occurrence of major rice insect pests at different transplanting times and fertilizer levels in paddy field. Korean J. Entomol. 35: 132-136.
  17. Oh SD, Lee KJ, Park SK, Park HM, Lee SM, Ahn HI, Sohn SI, Cho HS, Ryu TH. 2013. Biosafety assessment for drought-tolerant transgenic rice (CaMsrB2-8) : Progeny stability study for three generations. Korean J. Breed. Sci. 42: 157-162.
  18. Owen MDK. 2000. Current use of transgenic herbicide resistant soybean and corn in the USA, Crop Prot. 19: 765-771. https://doi.org/10.1016/S0261-2194(00)00102-2
  19. Qin Y, Kim SM, Park HY, Sohn JK. 2009. Inheritance analysis of giant mutation induced by T-DNA insertion in rice. Korean J. Breed. Sci. 41: 9-15.