Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells

  • Xia, Yi-Qun (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Wei, Xiao-Yan (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Li, Wu-Lan (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Kanchana, Karvannan (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Xu, Chao-Chao (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Chen, Da-Hui (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Chou, Pei-Hong (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Jin, Rong (Department of Digestive Diseases, The First Affiliated Hospital of Wenzhou Medical University) ;
  • Wu, Jian-Zhang (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University) ;
  • Liang, Guang (Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University)
  • Published : 2014.08.30
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Abstract

Curcumin and its analogues have been reported to exert anti-cancer activity against a variety of tumors. Here, we reported A501, a new curcumin analogue. The effect of A501 on cell viability was detected by MTT assay, the result showed that A501 had a better inhibiting effect on the four non-small cell lung cancer (NSCLC) cells than that of curcumin. Moreover, Colony forming experiment showed A501 significant restrained cell proliferation. Flow cytometry displayed A501 can cause G2/M arrest and induce apoptosis. Western blotting showed that A501 decreased the expression of cyclinB1, cdc-2, bcl-2, while increased the expression of p53, cleaved caspase-3 and bax. In conclusion, curcumin analogues A501 played antitumor activity by inhibiting cell proliferation and inducing apoptosis of NSCLC cells. And it was likely to be a promising starting point for the development of curcumin-based anticancer drugs.

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References

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