Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MiR-150-5p Suppresses Colorectal Cancer Cell Migration and Invasion through Targeting MUC4

  • Wang, Wei-Hua (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Chen, Jie (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Zhao, Feng (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Zhang, Bu-Rong (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Yu, Hong-Sheng (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Jin, Hai-Ying (Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University) ;
  • Dai, Jin-Hua (Department of Clinical Laboratory, Ningbo No.2 Hospital)
  • Published : 2014.08.15
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Abstract

Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.

Keywords

References

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