Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Intraspecific variation and geographic study of Lonicera insularis (Caprifoliaceae) based on chloroplast DNA sequences

엽록체 DNA를 이용한 섬괴불나무(Lonicera insularis Nakai)의 종내변이 및 지리학적 연구

  • Jeong, Keum Seon (Department of Biology Kyungpook National University) ;
  • Kim, Mi Sun (Department of Biology Kyungpook National University) ;
  • Lee, Woong (Department of Biology Kyungpook National University) ;
  • Pak, Jae-Hong (Department of Biology Kyungpook National University)
  • 정금선 (경북대학교 자연과학대학 생물학과) ;
  • 김미선 (경북대학교 자연과학대학 생물학과) ;
  • 이웅 (경북대학교 자연과학대학 생물학과) ;
  • 박재홍 (경북대학교 자연과학대학 생물학과)
  • Received : 2014.07.12
  • Accepted : 2014.09.16
  • Published : 2014.09.26
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Abstract

Lonicera insularis Nakai (Caprifoliaceae) is Korean endemic plant that lives along the shore of Ulleungdo and Dokdo. The aim of this study is to construct a phylogenetic relationship within six species (L.maackii, L.chrysantha, L.subsessilis, L. harai, L. morrowii) of genus Lonicera L. and Wigela subsessilis as outgroup and intraspecific variation of L. insularis using chloroplast DNA five regions sequences. Sequence analysis revealed that both L. insularis and L. morrowii showed complete homologies in the intergenic regions of trnL-trnF, trnS-trnG, psbM-trnD and matK coding region. However, sequence in the petN-psbM intergenic region showed a single nucleotide difference between both species, thus we designated them as CP01 and CP02. The plants having CP01 are prevalent in the Ulleungdo and Dokdo, while L. insularis and L. morrowii from Ulleungdo and of Dokdo, and Japan have CP02. This confirmed the existence of two cp DNA lineages with different geographical distributions. We can infer the allopatric speciation by geographical barrier. The result will provide the important basal data to study speciation and specie evolution of ocean islands such as Ulleungdo and Dokdo.

섬괴불나무(Lonicera insularis Nakai)는 인동과(Caprifoliaceae) 인동속(Lonicera L.) 에 속하는 관목으로 울릉도와 독도의 해안가 지역에 분포하는 한국특산식물이다. 섬괴불나무와 인동속의 근연종인 괴불나무, 청괴불나무, 각시괴불나무, 길마가지나무, L. morrowii, 병꽃나무 7분류군과의 유연관계를 밝히고, 섬괴불나무의 종내 변이를 확인하기 위해 엽록체 DNA 5개 영역의 염기서열을 분석하였다. 분석결과 전체 3.2kb의 염기서열이 결정되었고, 섬괴불나무와 L. morrowii 에서 2개의 엽록체 DNA haplotype(CP01-02)이 결정되었다. 섬괴불나무는 한 개의 염기치환으로 CP01 type과 CP02 type으로 구별되었으며, 일본의 L. morrowii와는 CP02 type을 공유하였다. 섬괴불나무에서 확인 된 두 개의 CP type은 1) 진화적으로 뚜렷한 두 개 이상의 계통을 가지며, 이는 하나 이상의 유입경로를 통해 울릉도로 유입되었을 가능성을 높게 지지한다. 그리고, 2) 형태학적으로 구별되는 섬괴불나무와 L. morrowii의 CP type의 공유는 두 종의 지리적인 장벽에 의한 이소적종분화의 결과로 추론할 수 있다. 본 연구에서 확인된 울릉도와 독도의 섬괴불나무의 종내 변이 및 다양성에 관한 결과는 울릉도 및 독도 식물의 분자생물지리학적 연구로 대양섬의 생물학적 진화양상과 종 분화 과정에 중요한 기초 자료로 활용될 것이다.

Keywords

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