Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Anti-inflammatory effects of Rubus coreanus Miquel through inhibition of NF-${\kappa}B$ and MAP Kinase

  • Lee, Jung Eun (Department of Home Economics Education, Chung-Ang University) ;
  • Cho, Soo-Muk (Functional Food & Nutrition Division, Rural Development Administration) ;
  • Park, Eunkyo (Department of Home Economics Education, Chung-Ang University) ;
  • Lee, Seung Min (Department of Food and Nutrition, College of Human Ecology, Yonsei University) ;
  • Kim, Yuri (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Auh, Joong Hyuck (Department of Food Science & Technology, Chung-Ang University) ;
  • Choi, Hyung-Kyoon (College of Pharmacy, Chung-Ang University) ;
  • Lim, Sohee (Department of Life Science, Chung-Ang University) ;
  • Lee, Sung Chul (Department of Life Science, Chung-Ang University) ;
  • Kim, Jung-Hyun (Department of Home Economics Education, Chung-Ang University)
  • Received : 2013.10.21
  • Accepted : 2013.12.28
  • Published : 2014.10.01
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Abstract

BACKGROUND/OBJECTIVES: Rubus Coreanus Miquel (RCM), used as a traditional Korean medicine, reduces chronic inflammatory diseases such as cancer and rheumatoid arthritis. However, its mechanism has not been elucidated. In this study, we examine the anti-inflammatory effects of RCM and their possible mechanisms using RAW 264.7 cells. MATERIALS/METHODS: Unripe RCM ethanol extract (UE), unripe RCM water extract (UH), ripe RCM ethanol extract (RE), and ripe RCM water extract (RH) were prepared. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (iNOS, COX-2, TNF-${\alpha}$, IL-$1{\beta}$, and IL-6) and NO and $PGE_2$ productions were assessed. To determine the anti-inflammatory mechanism of RCM, we measured NF-${\kappa}B$ and MAPK activities. RESULTS: UE and UH treatment significantly reduced NF-${\kappa}B$ activation and JNK and p38 phosphorylation and reduced transcriptional activities decreased iNOS, COX-2, and pro-inflammatory cytokines expressions, and NO and $PGE_2$ productions. RE and RH treatments reduced IL-$1{\beta}$ and IL-6 expressions through suppressions of JNK and p38 phosphorylation. CONCLUSIONS: In this study, we showed that RCM had anti-inflammatory effects by suppression of pro-inflammatory mediator expressions. Especially, unripe RCM showed strong anti-inflammatory effects through suppression of NF-${\kappa}B$ and MAPK activation. These findings suggest that unripe RCM might be used as a potential functional material to reduce chronic inflammatory responses.

Keywords

References

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