Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Inhibitory Effects of Dendropanax Morbifera Leaf Extracts on Melanogenesis through Down-Regulation of Tyrosinase and TRP-2

Tyrosinase 및 TRP-2의 발현 억제를 통한 황칠나무 잎 추출물의 Melanin 생성 저해 효과

  • Park, Su Ah (Department of Fine Chemistry, Nanobiocosmetic laboratory and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Lee, Hye Mi (Department of Fine Chemistry, Nanobiocosmetic laboratory and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Ha, Ji Hoon (Department of Fine Chemistry, Nanobiocosmetic laboratory and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Jeon, So Ha (Department of Fine Chemistry, Nanobiocosmetic laboratory and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Park, Soo Nam (Department of Fine Chemistry, Nanobiocosmetic laboratory and Cosmetic R&D center, Seoul National University of Science and Technology)
  • 박수아 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 이혜미 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 하지훈 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 전소하 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소)
  • Received : 2014.06.06
  • Accepted : 2014.08.14
  • Published : 2014.10.10
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Abstract

Dendropanax morbifera (D. morbifera) grows in the southern coastal areas and on Jeju Island in Korea. In this study, D. morbifera leaf extract was investigated to determine the mechanism of its whitening effect. The inhibitory activities of the extract on melanogenesis were tested in B16 melanoma cells treated with the ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH). D. morbifera leaf extracts remarkably decreased the melanin content at 25 and $50{\mu}g/mL$. The extracts significantly inhibited the intracellular tyrosinase activity and protein expression of tyrosinase and tyrosinase related protein-2 (TRP-2). In conclusion, D. morbifera leaf extracts would show a whitening effect by inhibiting intracellular tyrosinase activities and the expression of enzymes directly involved in the melanin biosynthesis. The results indicate that fractions of D. morbifera leaf extracts show potential for application as a whitening agent in the new whitening cosmetics.

황칠나무는 한국의 남부 해안 및 제주도 등에서 자생하는 식물이다. 본 연구에서는 황칠나무 잎 추출물의 미백 활성의 원인을 규명하고자 연구를 수행하였다. ${\alpha}$-MSH로 자극된 B16 melanoma 세포에 대한 추출물의 멜라닌 생합성 저해 활성을 측정하였다. 추출물은 25 및 $50{\mu}g/mL$ 농도에서 유의적으로 멜라닌 함량을 감소시켰으며, 세포 내의 tyrosinase 활성뿐만 아니라 tyrosinase와 TRP-2의 단백질 발현도 상당히 저해하였다. 결과적으로, 황칠나무 잎 추출물은 세포 내의 tyrosinase활성과 멜라닌 생합성에 직접적으로 관련된 효소의 발현을 저해함으로써 미백효과를 나타내었다. 본 연구 결과는 황칠나무 잎 추출물이 새로운 미백 화장품의 원료로써 이용 가능함을 시사한다.

Keywords

References

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