Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema

  • Kim, You-Sun (Asan Institute for Life Science) ;
  • Kim, Ji-Young (Asan Institute for Life Science) ;
  • Shin, Dong-Myung (Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Huh, Jin Won (Department of Pulmonary and Critical Care Medicine, Asthma Center, Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center) ;
  • Lee, Sei Won (Department of Pulmonary and Critical Care Medicine, Asthma Center, Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center) ;
  • Oh, Yeon-Mok (Asan Institute for Life Science)
  • Received : 2014.05.13
  • Accepted : 2014.06.24
  • Published : 2014.09.30
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Abstract

Background: Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema. Methods: We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection. Results: The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema. Conclusion: In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.

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References

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