Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Nutritional Composition and Cytoprotective Effect of Moringa oleifera Lam.

Drumstick-tree (Moringa oleifera Lam.)의 주요 영양성분 및 추출물의 신경세포 보호 효과

  • Jin, Su Il (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hyeon Ju (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji Hee (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 진수일 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김현주 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 정지희 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진동은 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2014.03.18
  • Accepted : 2014.07.17
  • Published : 2014.10.31
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Abstract

The cytoprotective effect of Moringa oleifera Lam. (drumstick tree) on neuronal cells was investigated to confirm the physiological benefits associated with this natural food resource. First, the drumstick tree extract was chemically analyzed to determine inherent nutritional constituents. Calcium and potassium were identified as the major mineral constituents, and palmitic acid (C16:0, 16.33%) and gadoleic acid (C20:01, 66.34%) were detected as the major fatty acids. Moreover, drumstick tree extract contained 94.78 mg/100 g vitamin E and 112.61 mg/100 g niacin. PC12 cells were used to study the cytoprotective effects of drumstick tree extract. Intracellular accumulation of reactive oxygen species was significantly reduced when $H_2O_2$ treated-neuronal cells were cultured in a medium containing the methanolic extract of drumstick tree, compared to cells treated with only $H_2O_2$. Cell viability assay using MTT showed that the extract protected cells against $H_2O_2$-induced neurotoxicity and inhibited LDH leakage from the cell membrane. Caspase assay showed that the extract exerted cytoprotective effect against apoptosis. Consequently, these data suggest that drumstick tree is a useful natural resource with positive effects on human health.

국내에 충분히 보고되지 못한 소재로서 drumstick-tree (Moringa Oleifera Lam.)를 고부가가치 식품 자원으로서 그 활용가능성을 알아보기 위해 주요 영양성분 분식 및 in vitro 신경세포 보호효과에 대해서 연구하였다. Drumstick-tree의 주요 무기성분으로는 칼슘으로 2658.67 mg/100 g이 함유되어 있었고, 다음으로 칼륨, 마그네슘 및 인 등이 함유되어 있었다. 주요 지방산으로서 포화 지방산으로는 palmitic acid (16.33%)와 불포화 지방산으로서 gadoleic acid (66.34%)가 상대적으로 많이 함유되어 있었고, 지용성 비타민인 vitamin E가 94.78 mg/100 g 그리고 niacin이 112.61 mg/100 g 함유되어 있는 것을 알 수 있었다. 80% methanol에 추출한 drum-stick-tree 추출물을 활용하여 $H_2O_2$ 처리한 PC12 cell 내의 활성산소 생성억제효과를 DCF-DA assay를 통해 측정한 결과 drumstick-tree 추출물은 농도의존적인 활성산소 생성 억제효과를 보였다. MTT assay를 이용하여 $H_2O_2$로 유도된 PC12 신경세포에 대한 보호효과를 측정한 결과 vitamin C group 대비 효과적인 신경세포 보호효과를 확인하였고, LDH release assay를 통해 일정 수준의 세포막 보호효과를 역시 확인하였다. 또한 PC12 cell의 oxidative stress-induced apoptosis에 대한 세포 보호효과를 측정하기 위한 caspase assay 실험 결과, 세포 내 caspase activity가 추출물의 의해 효과적으로 감소됨을 알 수 있었다. 결국 본 연구결과를 종합해 볼 때, 우수한 영양 구성 성분과 함께 신경세포 내 oxidative stress의 저감화 등을 통한 drumstick-tree 추출물의 신경 세포 보호 효과는 고부가가치 천연 소재로서의 다양한 산업적 활용 가능성을 암시하는 것으로 판단된다.

Keywords

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