Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Biochemical Characterization of α-Galactosidase-Producing Thermophilic Bacillus coagulans KM-1

α-Galactosidase를 생산하는 고온성 Bacillus coagulans KM-1 균주의 생화학적 특성

  • Nam, Ki Ho (Food and safety Research Division, National Fisheries Research and Development Institute) ;
  • Jang, Mi Soon (Food and safety Research Division, National Fisheries Research and Development Institute) ;
  • Park, Hee Yeon (Food and safety Research Division, National Fisheries Research and Development Institute) ;
  • Koneva, Elena (TINRO Centre, Pacific Scientific Research Fisheries Centre, Shevchenko Alley)
  • Received : 2014.09.22
  • Accepted : 2014.10.29
  • Published : 2014.10.31
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Abstract

A bacterium producing ${\alpha}$-galactosidase (${\alpha}$-$\small{D}$-galactoside galactohydrolase, EC 3.2.1.22) was isolated. The isolate, KM-1 was identified as Bacillus coagulans based on its 16S rRNA sequence, morphology, and biochemical properties. ${\alpha}$-Galactosidase activity was detected the culture supernatant of B. coagulans KM-1. The bacterium showed the maximum activity for hydrolyzing para-nitrophenyl-${\alpha}$-$\small{D}$-galactopyranoside (pNP-${\alpha}Gal$) at pH 6.0 and $50^{\circ}C$. It hydrolyzed oligomeric substrates such as melibiose, raffinose, and stachyose liberating a galactose residue, indicating that the B. coagulans KM-1 ${\alpha}$-galactosidase hydrolyzed ${\alpha}$-1,6 linkage. The results suggest that the decreased stachyose and raffinose contents in fermented soybean meal are due to the ${\alpha}$-galactosidase activity.

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References

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