Clinical and Experimental Otorhinolaryngology

Korean Society of Otolaryngology-Head and Neck Surgery (대한이비인후과학회)
권호 표지
DOI QR코드

DOI QR Code

Expression Profile of Fas-Fas Ligand in Spiral Ganglion Cells During Apoptosis

  • Gu, Tae Woo (Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Bae, Woo Yong (Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Park, Hwan Tae (Department of Physiology, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Lee, Jae Hoon (Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Kang, Min Young (Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Jeong, Sung Wook (Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine) ;
  • Shin, Yoon Kyung (Department of Physiology, Medical Science Research Institute, Dong-A University College of Medicine)
  • Received : 2012.09.14
  • Accepted : 2012.11.21
  • Published : 2014.03.30
⟨ Previous Next ⟩

Abstract

Objectives. To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis. Methods. Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining. Results. In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner. Conclusion. FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.

Keywords

Acknowledgement

Supported by : Dong-A University

References

  1. Puel JL. Chemical synaptic transmission in the cochlea. Prog Neurobiol. 1995 Dec;47(6):449-76. https://doi.org/10.1016/0301-0082(95)00028-3
  2. Pujol R, Puel JL, Gervais d'Aldin C, Eybalin M. Pathophysiology of the glutamatergic synapses in the cochlea. Acta Otolaryngol. 1993 May;113 (3):330-4. https://doi.org/10.3109/00016489309135819
  3. Janssen R, Schweitzer L, Jensen KF. Glutamate neurotoxicity in the developing rat cochlea: physiological and morphological approaches. Brain Res. 1991 Jun;552(2):255-64. https://doi.org/10.1016/0006-8993(91)90090-I
  4. Yuan J, Yankner BA. Apoptosis in the nervous system. Nature. 2000 Oct;407(6805):802-9. https://doi.org/10.1038/35037739
  5. Bae WY, Kim LS, Hur DY, Jeong SW, Kim JR. Secondary apoptosis of spiral ganglion cells induced by aminoglycoside: Fas-Fas ligand signaling pathway. Laryngoscope. 2008 Sep;118(9):1659-68. https://doi.org/10.1097/MLG.0b013e31817c1303
  6. Lee HK, Shin YK, Jung J, Seo SY, Baek SY, Park HT. Proteasome inhibition suppresses Schwann cell dedifferentiation in vitro and in vivo. Glia. 2009 Dec;57(16):1825-34. https://doi.org/10.1002/glia.20894
  7. Lee HK, Seo IA, Suh DJ, Hong JI, Yoo YH, Park HT. Interleukin-6 is required for the early induction of glial fibrillary acidic protein in Schwann cells during Wallerian degeneration. J Neurochem. 2009 Feb;108(3):776-86. https://doi.org/10.1111/j.1471-4159.2008.05826.x
  8. Ylikoski J, Wersall J, Bjorkroth B. Degeneration of neural elements in the cochlea of the guinea-pig after damage to the organ of corti by ototoxic antibiotics. Acta Otolaryngol Suppl. 1974;326:23-41.
  9. Lefebvre PP, Weber T, Rigo JM, Staecker H, Moonen G, Van De Water TR. Peripheral and central target-derived trophic factor(s) effects on auditory neurons. Hear Res. 1992 Mar;58(2):185-92. https://doi.org/10.1016/0378-5955(92)90127-9
  10. Kopke R, Staecker H, Lefebvre P, Malgrange B, Moonen G, Ruben RJ, et al. Effect of neurotrophic factors on the inner ear: clinical implications. Acta Otolaryngol. 1996 Mar;116(2):248-52. https://doi.org/10.3109/00016489609137834
  11. Staecker H, Kopke R, Malgrange B, Lefebvre P, Van de Water TR. NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells. Neuroreport. 1996 Mar;7(4):889-94. https://doi.org/10.1097/00001756-199603220-00011
  12. Krieglstein K, Strelau J, Schober A, Sullivan A, Unsicker K. TGF-beta and the regulation of neuron survival and death. J Physiol Paris. 2002 Jan-Mar;96(1-2):25-30. https://doi.org/10.1016/S0928-4257(01)00077-8
  13. Marzella PL, Gillespie LN, Clark GM, Bartlett PF, Kilpatrick TJ. The neurotrophins act synergistically with LIF and members of the TGF-beta superfamily to promote the survival of spiral ganglia neurons in vitro. Hear Res. 1999 Dec;138(1-2):73-80. https://doi.org/10.1016/S0378-5955(99)00152-5
  14. Ruel J, Chen C, Pujol R, Bobbin RP, Puel JL. AMPA-preferring glutamate receptors in cochlear physiology of adult guinea-pig. J Physiol. 1999 Aug;518(Pt 3):667-80. https://doi.org/10.1111/j.1469-7793.1999.0667p.x
  15. Jeong SW, Kim LS, Hur D, Bae WY, Kim JR, Lee JH. Gentamicin-induced spiral ganglion cell death: apoptosis mediated by ROS and the JNK signaling pathway. Acta Otolaryngol. 2010 Jun;130(6):670-8. https://doi.org/10.3109/00016480903428200
  16. Lee JE, Nakagawa T, Kim TS, Iguchi F, Endo T, Dong Y, et al. A novel model for rapid induction of apoptosis in spiral ganglions of mice. Laryngoscope. 2003 Jun;113(6):994-9. https://doi.org/10.1097/00005537-200306000-00015
  17. Alam SA, Ikeda K, Oshima T, Suzuki M, Kawase T, Kikuchi T, et al. Cisplatin-induced apoptotic cell death in Mongolian gerbil cochlea. Hear Res. 2000 Mar;141(1-2):28-38. https://doi.org/10.1016/S0378-5955(99)00211-7
  18. Alam SA, Oshima T, Suzuki M, Kawase T, Takasaka T, Ikeda K. The expression of apoptosis-related proteins in the aged cochlea of Mongolian gerbils. Laryngoscope. 2001 Mar;111(3):528-34. https://doi.org/10.1097/00005537-200103000-00026
  19. Watanabe K, Tomiyama S, Jinnouchi K, Yagi T. Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the hydropic cochlea of guinea pigs - second report. Eur Arch Otorhinolaryngol. 2002 May;259(5):257-61. https://doi.org/10.1007/s00405-002-0459-9
  20. Suda T, Nagata S. Purification and characterization of the Fas-ligand that induces apoptosis. J Exp Med. 1994 Mar;179(3):873-9. https://doi.org/10.1084/jem.179.3.873

Cited by

  1. Increased expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNA in chronic otitis media with cholesteatoma vol.134, pp.5, 2014, https://doi.org/10.3109/00016489.2013.878474
  2. Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells vol.15, pp.12, 2014, https://doi.org/10.7314/apjcp.2014.15.12.5023
  3. Effects of Memantine on Aminoglycoside-Induced Apoptosis of Spiral Ganglion Cells in Guinea Pigs vol.155, pp.1, 2014, https://doi.org/10.1177/0194599816639297