Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of a lipid-encapsulated zinc oxide dietary supplement, on growth parameters and intestinal morphology in weanling pigs artificially infected with enterotoxigenic Escherichia coli

  • Kim, Sung jae (College of Veterinary Medicine and Institute of Veterinary Science, National University) ;
  • Kwon, Chang Hoon (College of Veterinary Medicine and Institute of Veterinary Science, National University) ;
  • Park, Byung Chul (R & D Institute, Sunjin Co., Ltd.) ;
  • Lee, Chul Young (The Regional Animal Industry Center, Gyeongnam National University of Science and Technology) ;
  • Han, Jeong Hee (College of Veterinary Medicine and Institute of Veterinary Science, National University)
  • Received : 2014.10.17
  • Accepted : 2014.12.29
  • Published : 2015.01.31
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Abstract

The study was performed to investigate the effect of dietary supplementation of a lipid-encapsulated Zinc oxide on growth parameters and intestinal mucosal morphology piglets born to Duroc-sired Landrace ${\times}$ Yorkshire dams. Twenty-four 30-day-old piglets weaned at 25 days of age were orally challenged with $5{\times}10^8$ colony forming units of enterotoxigenic Escherichia coli (ETEC) K88 and fed one of the four diets for 7 days: (i) a nursery basal diet containing 100-ppm ZnO (referred to as BASAL), (ii) BASAL supplemented with 120-ppm apramycin (referred to as ANTIBIO), (iii) BASAL with 2,400-ppm ZnO (referred to as HIGH), and BASAL containing 100-ppm lipid-encapsulated ZnO (referred to as LE). All piglets were killed at the end of the experiment for histological examination on the intestine. The results showed that the average daily gain (ADG), the villus height: crypt depth (CD) ratio in the ileum, and the goblet cell density of the villus and crypt in the duodenum, jejunum, and colon were greater in the LE-fed group that those of the BASAL (p < 0.05). Fecal consistency score (FCS) and the CD ratio in the ileum were less in the LE-fed group, compared to the BASAL-fed one (p < 0.05). The effects observed in the LE-fed group were almost equal to those of the HIGH-fed group as well as even superior to those of the ANTIBIO-fed group. Taken together, our results imply that dietary supplementation of 100-ppm lipid-encapsulated ZnO is as effective as that of 2,400-ppm ZnO for promoting growth diarrhea and intestinal morphology caused by ETEC infection.

Keywords

Acknowledgement

Supported by : CTCBIO, Inc., Gyeongnam National University of Science and Technology

References

  1. Hill GM, Mahan DC, Carter SD, Cromwell GL, Ewan RC, Harrold RL, et al. Effect of pharmacological concentrations of zinc oxide with or without the inclusion of an antibacterial agent on nursery pig performance. J Anim Sci. 2001;79:934-41. https://doi.org/10.2527/2001.794934x
  2. Heo JM, Opapeju FO, Pluske JR, Kim JC, Hampson DJ, Nyachoti CM. Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds. J Anim Physiol Anim Nutr. 2013;97:207-37. https://doi.org/10.1111/j.1439-0396.2012.01284.x
  3. Hill GM. Minerals and mineral utilization in swine. In: Chiba LI, editor. Sustainable swine nutrition. Oxford, UK: John Wiley & Sons, Inc.; 2013. p. 173-95.
  4. Willing BP, Malick G, Van Kessel AG. Nutrition and gut health in swine. In: Chiba LI, editor. Sustainable swine nutrition. Oxford, UK: John Wiley & Sons, Inc.; 2013. p. 197-213.
  5. Madec F, Bridoux N, Bounaix S, Cariolet R, Duval-Iflah Y, Hampson DJ, et al. Experimental models of porcine post-weaning colibacillosis and their relationship to post-weaning diarrhoea and digestive disorders as encountered in the field. Vet Microbiol. 2000;72:295-310. https://doi.org/10.1016/S0378-1135(99)00202-3
  6. Wellock IJ, Fortomaris PD, Houdijk JGM, Kyriazakis I. Effects of dietary protein supply, weaning age and experimental enterotoxigenic Escherichia coli infection on newly weaned pigs: health. Animal. 2008;2:834-42.
  7. Slade RD, Kyriazakis I, Carroll SM, Reynolds FH, Wellock IJ, Broom LJ, et al. Effect of rearing environment and dietary zinc oxide on the response of group-housed weaned pigs to enterotoxigenic Escherchia coli O149 challenge. Animal. 2011;5:1170-8. https://doi.org/10.1017/S1751731111000188
  8. Kim JC, Hansen CF, Pluske JR, Mullan BP. Evaluating the replacement of zinc oxide with an encapsulated zinc oxide product as a means of controlling post-weaning diarrhea in piglets. Project 2C-114. Pork CRC. 2010. p. 1-16.
  9. Kwon CH, Lee CY, Han SJ, Kim SJ, Park BC, Jang I, et al. Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis, growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli. Anim Sci J. 2014;85:805-13. https://doi.org/10.1111/asj.12215
  10. Heo JM, Kim JC, Hansen CF, Mullan BP, Hampson DJ, Pluske JR. Feeding a diet with decreased potein content reduces indices of protein fermentation and the incidence of postweaning diarrhea in weaned pigs challenged with an enterotoxigenic strain of Escherichia coli. J Anim Sci. 2009;87:2833-43. https://doi.org/10.2527/jas.2008-1274
  11. Yan L, Jang HD, Kim IH. Effects of varying creep feed duration on preweaning and post-weaning performance and behavior of piglet and sow. Asian-Aust J Anim Sci. 2011;24:1601-6. https://doi.org/10.5713/ajas.2011.11181
  12. Zhao PY, Jung JH, Kim IH. Effect of mannan oligosaccharides and fructan on growth performance, nutrient digestibility, blood profile, and diarrhea score in weanling pigs. J Anim Sci. 2012;90:833-9. https://doi.org/10.2527/jas.2011-3921
  13. Lee CY, Lim Y-W, Ko Y-H, Kang S-Y, Park MJ, Ko T, et al. Intestinal growth and deveopment of weanling pigs in response to dietay supplementation of antibiotics, phytogenic products and brewer’s yeas plus Bacillus spores. J Anim Sci Technol. 2011;53:227-35. https://doi.org/10.5187/JAST.2011.53.3.227
  14. West DM, Sprigings KA, Sassar C, Wakeley PR, Sawyer J, Davies RH. Rapid detection of Escherichia coli virulence factor genes using multiplex real-time $TaqMan^{(R)}$ PCR assays. Vet Microbiol. 2007;122:323-31. https://doi.org/10.1016/j.vetmic.2007.01.026
  15. Uni Z, Smirnov A, Sklan D. Pre- and posthatch development of goblet cells in the broiler small intestine: effect of delayed access to feed. Poultry Sci. 2003;82:320-7. https://doi.org/10.1093/ps/82.2.320
  16. Jin Z, Yang YX, Choi JY, Shinde PL, Yoon SY, Hahn T-W, et al. Potato (Solanum tuberosum L. cv. Gogu valley) protein as a novel antimicrobial agent in weanling pigs. J Anim Sci. 2008;86:1562-72. https://doi.org/10.2527/jas.2007-0414
  17. Lee JS, Awji EG, Lee SJ, Tassew DD, Park YB, Park KS, et al. Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weanling pigs challenged with enterotoxigenic Esherichia coli. J Anim Sci. 2012;90:3709-17. https://doi.org/10.2527/jas.2011-4434
  18. Owusu-Asiedu A, Nyachoti CM, Marquardt RR. Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic. J Anim Sci. 2003;81:1790-8. https://doi.org/10.2527/2003.8171790x
  19. Li BT, Van Kessel AG, Caine WR, Huang SX, Kirkwood RN. Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietay level of zinc oxide. Can J Anim Sci. 2001;81:511-6. https://doi.org/10.4141/A01-043
  20. Li X, Yin J, Li D, Chen X, Zang J, Zhou X. Dietary supplementation with zinc oxide increases IGF-I and IGF-I receptor gene expression in the small intestine of weanling piglets. J Nutr. 2006;136:1786-91. https://doi.org/10.1093/jn/136.7.1786
  21. Hedemann MS, Jensen BB, Poulsen HD. Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs. J Anim Sci. 2006;84:3310-20. https://doi.org/10.2527/jas.2005-701

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