Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Attenuation of Diabetic Conditions by Sida rhombifolia in Moderately Diabetic Rats and Inability to Produce Similar Effects in Severely Diabetic in Rats

  • Received : 2015.06.17
  • Accepted : 2015.11.11
  • Published : 2015.12.31
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Abstract

Objectives: This study was done out to evaluate the effects of Sida rhombifolia methanol extract (SRM) on diabetes in moderately diabetic (MD) and severely diabetic (SD) Sprague-Dawley rats. Methods: SRM was prepared by soaking the powdered plant material in 70% methanol and rota evaporating the methanol from the extract. Effective hypoglycemic doses were established by performing oral glucose tolerance tests (OGTTs) in normal rats. Hourly effects of SRM on glucose were observed in the MD and the SD rats. Rats were grouped, five rats to a group, into normal control 1 (NC1), MD control 1 (MDC1), MD experimental 1 (MDE1), SD control 1 (SDC1), and SD experimental 1 (SDE1) groups. All rats in the control groups were administered 1 mL of distilled water (DW). The rats in the MDE1 and the SDE1 groups were administered SRM orally at 200 and 300 mg/kg body weight (BW), respectively, dissolved in 1 mL of DW. Blood was collected initially and at intervals of 1 hour for 6 hours to measure blood glucose. A similar experimental design was followed for the 30-day long-term trial. Finally, rats were sacrificed, and blood was collected to measure blood glucose, lipid profiles, thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH). Results: OGTTs indicated that two doses (200 and 300 mg/kg BW) were effective hypoglycemic doses in normal rats. Both doses reduced glucose levels after 1 hour in the MDE1 and the SDE1 groups. A long-term trial of SRM in the MD group showed a reduced glucose level, a normal lipid profile, and normal GSH and TBARS levels. In SD rats, SRM had no statistically significant effects on these parameters. Normal weight was achieved in the MD rats, but the SD rats showed reduced BW. Conclusion: The study demonstrates that SRM has potential to alleviate the conditions of moderate diabetic, but not severe diabetes.

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References

  1. Wolf SP, Crabbe MJC, Thornalley PJ. The autooxidation of glyceraldehydes and other simple monosaccharide. Experientia. 1984;40(3):244-6. https://doi.org/10.1007/BF01947562
  2. Ramasamy R, Vannucci SJ, Yan SS, Herold K, Yan SF, Schmidt AM. Advanced glycation end products and RAGE: a common thread in aging, diabetes, neurodegeneration, and inflammation. Glycobiology. 2005;15(7):16-28. https://doi.org/10.1093/glycob/cwi053
  3. Vlassara H, Palace MR. Diabetes and advanced glycation endproducts. J Intern Med. 2002;251(2):87-101. https://doi.org/10.1046/j.1365-2796.2002.00932.x
  4. Chaturvedi P, Batisane B. Acute effect of some organic solvent extracts of Melia azedarach fruit on glucose levels in albino rats. J Appl Zool Res. 2005;16(2):238-41.
  5. Chaturvedi P. Role of Momordica charantia in maintaining the normal glucose level and lipid profile in diabetic rats fed on high fat and low carbohydrate diet. Br J Biomed Sci. 2005;62(3):124-6. https://doi.org/10.1080/09674845.2005.11732698
  6. Chaturvedi P, Kwape T, George S. Effects of Sida rhombifolia on lipid profile and glucose tolerance in diabetic rats. J Appl Zool Res. 2009;20(2):161-7.
  7. Subramanya MD, Pai SR, Upadhya V, Ankad GM, Bhagwat SS, Hedge HV. Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India. J Ayurveda Integr Med. 2015;6(1):24-8. https://doi.org/10.4103/0975-9476.146544
  8. Dhalwal K, Shinde VM, Singh B, Mahadik KR. Hypoglycemic and hypolipedaemic effects of Sida rhombifolia ssp. Retusa in diabetic induced animals. Int J Phytomed. 2010;2(2):160-5. https://doi.org/10.5138/ijpm.2010.0975.0185.02025
  9. Ahmad M, Prawez S, Sultana M, Raina R, Pankaj NK, Verma PK, et al. Anti-hyperglycemic, anti-hyperlipidemic and antioxidant potential of alcoholic extract of Sida cordifolia (areal part) in streptozotocin induced diabetes in wistar rats. Proc Natl Acad Sci. 2014;84(2):397-405.
  10. Sushmakumari S, Jaydeep A, Kumar JS, Manon VP. Effect of carnitine on malonyldialdehyde, taurine and glutathione levels in heart of rats subjected to myocardial stress by isoprotenol. Indian J Exp Biol. 1989;27(2):134-7.
  11. Ellman GC. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70-7. https://doi.org/10.1016/0003-9861(59)90090-6
  12. Krauss RM. Lipids and lipoproteins in patients with type 2 diabetes. Diabetes Care. 2004;27(6):1496-504. https://doi.org/10.2337/diacare.27.6.1496
  13. Takahiro O, Chikao S, Yutaka H, Noriaki K, Kenji I, Yoshihiko N, et al. Hyperlipidemia and fat absorption in model rats with type 2 diabetes mellitus. Bulletin of the Osaka Medical College. 2006;52(2):45-58.
  14. Hopkins GJ, Barter PJ. Role of triglyceride-rich lipoproteins and hepatic lipase in determining the particle size and composition of high density lipoproteins. J Lipid Res. 1986;27(12):1265-77.
  15. Kim HJ, Kurup JV. Non enzymatic glycosylation of human plasma low density lipoprotein. evidence for in vitro and in vivo glycosylation. Metabolism. 1982;31:348-53. https://doi.org/10.1016/0026-0495(82)90109-3
  16. Jeppesen J, Hein HO, Suadicani P, Gyntelberg F. Relation of high TG-low HDL cholesterol and LDL cholesterol to the incidence of ischemic heart disease: an 8-year follow-up in the Copenhagen male study. Arterioscler Thromb Vasc Biol. 1997;17(6):1114-20. https://doi.org/10.1161/01.ATV.17.6.1114
  17. Zambon A, Bertocco S, Vitturi N, Polentarutti V, Vianello D, Crepaldi G. Relevance of hepatic lipase to the metabolism of triacylglycerol-rich lipoproteins. Biochem Soc Trans. 2003;31(5):1070-4. https://doi.org/10.1042/bst0311070
  18. Peerapatdit T, Patchanans N, Likidlilid A, Poldee S, Sriratnassathavorn C. Plasma lipid peroxidation and antioxidant nutrients in type 2 diabetes patients. J Med Assoc Thai. 2006;89(S5):147-55.
  19. Wolf G, Muller N, Mandecka A, Muller UA. Association of diabetic retinopathy and renal function in patients with type 1and 2 diabetes mellitus. Clin Nephrol. 2007;68(2):81-6. https://doi.org/10.5414/CNP68081
  20. Memisogullari R, Taysi S, Bakan E, Capoglu I. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cell Biochem Funct. 2003;21(3):291-6. https://doi.org/10.1002/cbf.1025
  21. Sekhar RV, McKay SV, Patel SG, Guthikonda AP, Reddy VT, Balasubramanyam A, et al. Glutathione synthesis is diminished in patients with uncontrolled diabetes and restored by dietary supplementation with cysteine and glycine. Diabetes Care. 2011;34(1):162-7. https://doi.org/10.2337/dc10-1006
  22. Chaturvedi P, Kwape TE, Fulukani I . Evaluation of free radical scavenging activities of Sida rhombifolia extracts. Indian J Plant Sci. 2015;4(1):5-10.
  23. Dhalwal K, Despandey YS, Purohit AP. Evaluation of in vitro antioxidant activity of Sida rhombifolia (L.) ssp retusa (L.). Journal of Medicinal food. 2007;10(4):683-8. https://doi.org/10.1089/jmf.2006.129
  24. Bahadoran Z, Mirmiran P, Azizi F. Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J Diabetes Metab Disord. 2013;12(1):43. https://doi.org/10.1186/2251-6581-12-43