Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Obesity Effect of Fermented Detoxified Rhus verniciflua Vinegar Supplementation in Diet-Induced Obese Rats

무독화한 옻발효초가 고지방식이를 급여한 흰쥐에 미치는 항비만 효과

  • Cheong, So Ra (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Ranseon (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Yoo Kyoung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Baek, SeongYeol (Fermented Food Science Division, Department of Agro-food Resource, NAAS, RDA) ;
  • Yeo, Soo-Hwan (Fermented Food Science Division, Department of Agro-food Resource, NAAS, RDA) ;
  • Lee, ChoongHwan (Department of Bioscience and Biotechnology, Konkuk University)
  • 정소라 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김란선 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박유경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 백성열 (국립농업과학원 발효식품과) ;
  • 여수환 (국립농업과학원 발효식품과) ;
  • 이충환 (건국대학교 생명공학과)
  • Received : 2015.09.08
  • Accepted : 2015.10.06
  • Published : 2015.12.31
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Abstract

The study aimed to investigate the anti-obesity effects of 1% Rhus verniciflua vinegar (RV) supplementation in high-fat-diet (60% fat)-induced obese rats. A total of 50 4-wk-old male Sprague-Dawley rats were fed normal chow diet or maintained on high-fat diet (HFD) for 12 weeks to induce obesity and were then randomized into five groups as follows: normal diet+ultra-pure water (CON), HFD+ultra-pure water (OB-DW), HFD+1% acetic acid (OBAA), HFD+1% RV (OB-RV), and HFD+0.1% caffeine (OB-CF). AA was used as a control for RV, and caffeine was used as a positive control due to its weight reducing effect. After 2 months, body weight, organ and adipose tissue weights, serum lipids, hepatic lipids, adipocyte size, and cell number per spot level were analyzed. As a result, food efficiency ratio, abdominal adipose tissue weight, serum levels of total cholesterol, triacylglycerol, free fatty acids, coronary artery index, and fecal lipid were significantly reduced in the RV treatment group. In this study, we found that dietary RV improved obesity by increasing lipid excretion and reducing lipogenesis. These results suggest that RV has potential as a functional anti-obesity food.

본 연구에서는 고지방식이로 비만 유도된 쥐를 대상으로 10주간 옻식초를 급여하였을 때 비만에 미치는 영향을 알아보고자 하였다. 옻식초는 무독화된 옻을 이용하여 알코올 발효 후 아세트산 발효를 거쳐 제조하였으며, 흰쥐를 일반식이(normal chow diet)와 고지방식이(fat 60 %kcal) 섭취군으로 나누어 12주 동안 비만 유도를 한 후 총 5군으로 나누었다. 일반식이와 정제수를 음용한 CON군, 고지방식이와 정제수를 음용한 OB-DW군, 고지방식이와 1% 아세트산을 음용한 OB-AA군, 고지방식이와 1% 옻식초를 음용한 OBRV군, 고지방식이와 0.1% 카페인 용액을 음용한 OB-CF군으로 나누어 총 10주 동안 해당 식이와 실험시료를 공급하였으며, 연구 종료 후 체중, 지방 무게, 지방구 크기 및 개수, 혈액과 간 조직의 지질 profile, 지방조직의 glycerol-3-phosphate dehydrogenase와 lipoprotein lipase(LPL) 효소 활성을 분석하였다. 그 결과 OB-DW군과 비교하여 OBRV군에서 체중 증가량과 식이효율이 유의적으로 감소하였고, 혈중 총 콜레스테롤과 중성지방 함량, 심장동맥경화지수(CRI), LPL 활성이 유의적으로 감소하였으며, 또한 혈중 유리지방산과 분변 중성지방의 함량이 유의성 있게 증가하였다(P<0.05). 항비만 효과를 살펴보기 위해 비만 흰쥐에게 옻식초를 음용시켰을 때 혈청이나 간의 지질 함량을 개선시키고 지질 배설을 증가시켜 부작용 없이 식품소재로서 비만을 일부 개선시키는 효과를 규명하였다.

Keywords

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