Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on Preparation of Water in Oil in Water (W1/O/W2) Emulsion Containing Titrated Extract of Centella asiatica

센텔라 아시아티카 정량추출물을 함유한 Water in Oil in Water (W1/O/W2) 에멀젼 제조에 관한 연구

  • 서동환 (코스맥스 R&I center) ;
  • 이홍선 (아주대학교 대학원 응용생명공학과 화장품과학전공) ;
  • 윤종혁 (코스맥스 R&I center) ;
  • 김연준 (코스맥스 R&I center) ;
  • 변상요 (아주대학교 대학원 응용생명공학과 화장품과학전공)
  • Received : 2015.12.01
  • Accepted : 2015.12.15
  • Published : 2015.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Titrated extract of Centella asiatica (TECA), which is poorly soluble in water is well known for wound healing and anti wrinkle agent. This study was conducted to find the optimum condition for the preparation of water in oil in water ($W_1/O/W_2$) emulsion containing TECA. Solubility of TECA were measured by UV spectrophotometer. 2.55 g of TECA was dissolved in solution composed of dipropylene glycol (40.0 g), ethanol (20.0 g), and water (10.0 g). Factors affecting stability of the emulsions ($W_1/O$, $W_1/O/W_2$) was investigated. The optimum conditions for the preparation of $W_1/O$ emulsion was composed of dipropylene glycol : ethanol : water : TECA in a weight ratio of 40.0 : 20.0 : 10.0 : 2.5 for water phase and squalane : cetyl PEG/PPG-10/1 dimethicone : cetearyl alcohol in a weight ratio of 22.5 : 4.0 : 2.5 for oil phase. The optimum conditions for the preparation of $W_1/O/W_2$ multiple emulsion was composed of water : $W_1/O$ emulsion : polysorbate 80 : carbomer : triethanolamine in a weight ratio of 55.8 : 40.0 : 4.0 : 0.1 : 0.1.

센텔라 아시아티카 정량추출물(TECA)은 병풀에서 얻은 난용성 추출물로 상처치유 및 항주름 물질로 알려져 있다. 본 연구에서는 TECA를 함유한 water in oil in water ($W_1/O/W_2$) 에멀젼 제조에 필요한 최적의 실험 조건을 찾기 위해 연구를 진행하였다. TECA의 용해도는 UV 흡광도계를 이용하였으며, 디프로필렌글라이콜(40.0 g), 에탄올(20.0 g) 및 정제수(10.0 g)의 조성에서 2.55 g의 TECA가 용해되는 것을 확인하였다. 에멀젼($W_1/O$, $W_1/O/W_2$)의 안정도에 영향을 미치는 요인에 대하여 조사하였다. $W_1/O$ 에멀젼의 제조하기 위한 최적의 수상 조건은 디프로필렌글라이콜 : 에탄올 : 정제수 : TECA가 40.0 : 20.0 : 10.0 : 2.5%(w/w), 유상 조건은 스쿠알란 : 세틸피이지/피피지-10/1디메치콘 : 세테아릴알코올이 22.5 : 4.0 : 1.0%(w/w)임을 확인하였다. $W_1/O/W_2$ 멀티에멀젼을 제조하기 위한 최적의 조건은 정제수 : $W_1/O$ 에멀젼 : 폴리소르베이트 80 : 카보머 : 트리에탄올아민의 비율이 55.8 : 40.0 : 4.0 : 0.1 : 0.1% (w/w)임을 확인하였다.

Keywords

References

  1. I. D. Kim, R. H. Kwon, Y. Y. Heo, H. J. Jung, H. Y. Kang, and B. J. Ha, Supercritical extraction of oriental herb: anti-aging and anti-wrinkles effects, Kor. J. Biotechnol. Bioeng., 23(6), 529 (2008).
  2. E. J. Lee, The development of anti-wrinkle cosmeceutical ingredients from glehniae radix cum rhizoma, Master's Thesis Dissertation, Chung Ang Univ., Seoul, Korea (2010).
  3. Y. K. Jo, The development of anti-wrinkle cosmeceutical ingredients from leonuri herba extrat, Master's Thesis Dissertation, Chung Ang Univ., Seoul, Korea (2011).
  4. S. Lee, Novel nantural products as active material for beauty food, J. Food Sci., 40, 10 (2008).
  5. M. S. Lim, M. A Pack, and S. N. Park, Preparation of nano-emulsion containing Polygonum aviculare extract for enhanced transdermal delivery, Appl. Chem. Eng., 23(2), 222 (2012).
  6. D. W. Kim, M. H. Cho, S. Y. Park, J. H. Lee, G. W. Lee, M. S. Park, J. K. Park, and U. K. Jee, Preparation and evaluation of titrated extract of Centella asiatica niosome/w/o system cream for site specific targeting, J. Kor. Pharm. Sci., 32(4), 291 (2002).
  7. A. Segall, M. Sosa, A. Alami, C. Enero, F. Hormaechea, M. T. Pizzorno, C. Bregni, and R. Serrao, Stability study of lipoic acid in the presence of vitamins A and E in o/w emulsions for cosmetic application, J. Cosmet. Sci., 55, 449 (2004).
  8. E. Touitou, N. Dayana, L. Bergelsonb, B. Godina, and M. Eliaza, Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties, J. of Controlled Release, 65(3), 403 (2000). https://doi.org/10.1016/S0168-3659(99)00222-9
  9. A. C. Williams and B. W. Barry, Penetration enhancers, Advanced Drug Delivery Reviews, 64, 128 (2012). https://doi.org/10.1016/j.addr.2012.09.032
  10. K. J. Lissant, Structure of high-internal-phase-ratio emulsions, J. Colloid Interface Sci., 47(2), 416 (1974). https://doi.org/10.1016/0021-9797(74)90273-2
  11. W. H. Kim, K. S. Lee, and K. K. Lee, An experimental study on the property and stability of W/O emulsion by various structures of emulsifier, J. Soc. Cosmet. Scientists Korea, 38(2), 119 (2012). https://doi.org/10.15230/SCSK.2012.38.2.119
  12. J. Y. Yeon, B. R. Shin, T. G. Kim, J. M. Seo, C. H. Lee, S. G. Lee, and H. B. Pyo, A study on emulsion stability of O/W and W/S emulsion according to HLB of emulsifier, J. Soc. Cosmet. Scientists Korea, 40(3), 227 (2014). https://doi.org/10.15230/SCSK.2014.40.3.227
  13. E. J. Lim, A study on the release of electrolyte from W/O/W multiple emulsion stabilized with xanthan gum and cetostearyl alcohol, Master's Thesis Dissertation, Kon Kuk Univ., Seoul, Korea (1995).
  14. C. K. Zhoh, K. Y. Lee, and D. N. Kim, The influences of fatty alcohol and fatty acid on rheological properties of o/w emulsion, J. Soc. Cosmet. Scientists Korea, 35(2), 103 (2009).
  15. R. Jantharaprapap and G. Stagni, Effects of penetaration enhancers on in vitro permeability of meloxicam gels, Int. J. Pharm., 343, 26 (2007). https://doi.org/10.1016/j.ijpharm.2007.04.011
  16. W. Liu, D. Sun, C. Li, Q. Liu, and J. Xu, Formation and stability of paffin oil-in-water nano-emulsions prepared by the emulsion inversion point method, J. Colloid. Interface. Sci., 303, 557 (2006). https://doi.org/10.1016/j.jcis.2006.07.055
  17. N. Anarjan and C. P. Tan, Effects of selected polysorbate and sucrose ester emulsifiers on the physicochemical properties of astaxanthin nanodispersions, Molecules, 18, 768 (2013). https://doi.org/10.3390/molecules18010768
  18. C. Anchisi, A. M. Maccioni, C. Sinico, and D. Valenti, Stability studies of new cosmetic formulations with vegetable extracts as functional agents, Il Farmaco, 56, 427 (2001). https://doi.org/10.1016/S0014-827X(01)01055-2