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Evaluation of Pea (Pisum sativum L.) Genetic Resources for Sprout and Analysis of Optimum Culture Condition for Pea Sprout

완두나물 재배에 적합한 유용자원 선발과 완두나물 적정 재배조건 설정

  • 구성철 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 강범규 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김현태 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 서정현 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 정광호 (농촌진흥청 국립식량과학원 기획조정과) ;
  • 윤홍태 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 오인석 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 최만수 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과)
  • Received : 2015.08.19
  • Accepted : 2015.10.05
  • Published : 2015.12.31

Abstract

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and $25^{\circ}C$). However, whole length, hypocotyl length, and sprout yield were highly increased at $23^{\circ}C$ compared to 20 and $25^{\circ}C$. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at $23^{\circ}C$ than at 20 and $25^{\circ}C$, while antioxidant properties of PI343278 and PI343283 were decreased in a temperature-dependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.

본 연구는 완두나물로 활용 가능한 유전자원을 선발하고, 그에 적합한 재배조건을 설정하고자 재배 온도 및 기간에 따른 완두나물의 재배특성 및 항산화 활성을 분석하여 완두를 이용한 채소용 나물 최적 재배 조건을 구명하고자 실시하였다. 1. 완두 유전자원 38점 중 완두 나물로 이용 가능한 자원으로 PI269803, PI343278, PI343283, PI343300와 PI343307을 1차적으로 선발하였다. 2. 1차 선발된 완두자원을 대상으로 완두나물 재배 조건을 설정하기 위하여 $20^{\circ}C$, $23^{\circ}C$$25^{\circ}C$에서 각각 7일간 재배하였다. 그 결과 전장과 배축장은 $20^{\circ}C$$25^{\circ}C$에 비해 $23^{\circ}C$에서 가장 높게 증가하였다. 3. 재배온도별 나물수율은 $23^{\circ}C$에서 가장 높게 나타났으며, 유전자원 PI269803와 PI343300이 다른 자원에 비해 높은 나물수율을 나타내었다. 4. 재배온도별 완두나물의 조단백질 함량을 분석한 결과 온도에 따른 조단백질 변화는 나타나지 않았다. 5. 재배 온도별 완두나물의 폴리페놀 함량과 자유 라디칼 소거능을 측정함으로써 항산화 특성을 조사하였다. 그 결과 PI343278와 PI343283에서는 재배 온도가 증가함에 따라 폴리페놀 함량과 자유 라디칼 소거능이 감소하는 경향을 나타내었다. 그러나 PI269803와 PI343300에서는 $20^{\circ}C$$25^{\circ}C$에 비해 $23^{\circ}C$에서 높은 폴리페놀 함량과 항산화 활성을 나타내었다. 6. 높은 나물수율과 항산화 특성을 보이는 PI269803과 PI343300은 완두나물 이용에 적합한 자원으로 예상된다.

Keywords

References

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