Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Zerumbone's Effects on Jurkat Cell Proliferation and Migration

Zerumbone이 Jurkat 세포의 증식과 유주에 미치는 영향

  • Moon, Cheol (Department of Clinical Laboratory Science, Semyung University)
  • 문철 (세명대학교 임상병리학과)
  • Received : 2015.10.30
  • Accepted : 2015.11.13
  • Published : 2015.12.30
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Abstract

Zerumbone is a major component of the essential oils of Zingiber zerumbet Smith and is known to have a number of effects on the functions of various cells, including immune cells. Many reports present the zerumbone's functions in various biological environments including cancer and inflammation. In this report, using a transwell system, we confirmed that zerumbone decreased the stromal cell-driven factor-$1{\alpha}$ (SDF-$1{\alpha}$), induced migration of Jurkat cells; about a 25% decrease in the case of 100 ng/mL SDF-$1{\alpha}$ treatment, 17% decrease in the case of 200 ng/mL. Whereas, no significant changes of basic cellular proliferation were observed after zerumbone treatment. These results are novel and promising functions of zerumbone on T cell physiology. At the same time, there is a great need to confirm the results using more physiological T cells and to proceed with cellular and biochemical mechanism studies, measuring apoptosis, CXCR4 expression and phosphorylation of ZAP-70 and Erk1/2.

야생 생강 Zingiber zerumbet Smith의 정유에 포함되어 있는 주요 성분인 zerumbone은 면역세포를 포함한 여러 종류의 세포기능에 영향을 미치는 것으로 알려져 있다. 또한, 종양, 염증을 포함한 여러 생물학적 환경에서 기능을 나타냄이 보고되었다. 본 연구에서는 zerumbone이 SDF-$1{\alpha}$로 유도된 T림프구 세포주 Jurkat 세포의 이동을 감소시키는 것을 transwell system을 이용하여 확인하였다; 100 ng/mL의 SDF-$1{\alpha}$로 유발시킨 이동은 약 25%, 200 ng/mL의 SDF-$1{\alpha}$로 유발시킨 경우에는 약 17%의 감소를 나타냈다. 반면에, Jurkat 세포의 기본 증식에는 큰 변화를 유발하지 않는다는 점을 WST assay를 통해 확인하였다. 이는 zerumbone이 지닌 새로운 기능이지만, 향후 생리적 세포를 통해 다시 확인해야 하며, zerumbone에 의한 세포자멸사 유발, CXCR4 발현 감소 등 세포학적 기전 연구와 ZAP-70, Erk1/2의 인산화 변화 측정 등 생화학적 기전 연구가 필요하다.

Keywords

References

  1. Abdelwahab SI, Abdul AB, Mohan S, Taha MM, Ibrahim MY, Mariod AA. Zerumbone induces apoptosis in T-acute lymphoblastic leukemia cells. Leuk Res. 2011, 35(2):268-271. https://doi.org/10.1016/j.leukres.2010.07.025
  2. Alampour-Rajabi S, El Bounkari O, Rot A, Muller-Newen G, Bacheleri F, Gawaz M, et al. MIF interacts with CXCR7 to promote receptor internalization, ERK1/2 and ZAP-70 signaling, and lymphocyte chemotaxis. Faseb J. 2015, 29(11):4497-4511 https://doi.org/10.1096/fj.15-273904
  3. Bordon Y. Lymphocyte migration: Travel agents for two. Nat Rev Immunol. 2011, 11(2):77-88.
  4. Eguchi A, Kaneko Y, Murakami A, Ohigashi H. Zerumbone suppresses phorbol ester-induced expression of multiple scavenger receptor genes in THP-1 human monocytic cells. Biosci Biotechnol Biochem. 2007, 71(4):935-45. https://doi.org/10.1271/bbb.60596
  5. Keong YS, Alitheen NB, Mustafa S, Abdul Aziz S, Abdul Rahman M, Ali AM. Immunomodulatory effects of zerumbone isolated from roots of Zingiber zerumbet. Pak J Pharm Sci. 2010, 23(1):75-82.
  6. Kitayama T, Iwabuchi R, Minagawa S, Shiomi F, Cappiello J, Sawada S, et al. Unprecedented olefin-dependent histidinekinase inhibitory of zerumbone ring-opening material. Bioorg Med Chem Lett. 2004, 23:5943-5946.
  7. Murakami A, Hayashi R, Tanaka T, Kwon KH, Ohigashi H, Safitri R. Suppression of dextran sodium sulfate-induced colitis in mice by zerumbone, a subtropical ginger sesquiterpene, and nimesulide: separately and in combination. Biochem Pharmacol. 2003, 66(7):1253-1261. https://doi.org/10.1016/S0006-2952(03)00446-5
  8. Murakami A, Ohigashi H. Cancer-preventive anti-oxidants that attenuate free radical generation by inflammatory cells. Biol Chem. 2006, 387(4):387-392. https://doi.org/10.1515/BC.2006.052
  9. Murakami A, Song M, Katsumata S, Uehara M, Suzuki K, Ohigashi H. Citrus nobiletin suppresses bone loss in ovariectomized ddY mice and collagen-induced arthritis in DBA/1J mice: possible involvement of receptor activator of NF-kappaB ligand (RANKL)-induced osteoclastogenesis regulation. Biofactors. 2007, 30(3):179-192. https://doi.org/10.1002/biof.5520300305
  10. Park YR, Moon C, Kim SH, Lee PJ. Induction of HaCaT cell apoptosis by sodium nitroprusside. Korean J Clin Lab Sci. 2015, 47(3):112-116. https://doi.org/10.15324/kjcls.2015.47.3.112
  11. Perimal EK, Akhtar MN, Mohamad AS, Khalid MH, Ming OH, Khalid S, et al. Zerumbone-induced antinociception: involvement of the L-arginine-nitric oxide-cGMP -PKC-K+ ATP channel pathways. Basic Clin Pharmacol Toxico. 2011, l108(3):155-162.
  12. Shanmugam MK, Rajendran P, Li F, Kim C, Sikka S, Siveen KS, et al. Abrogation of STAT3 signaling cascade by zerumbone inhibits proliferation and induces apoptosis in renal cell carcinoma xenograft mouse model. Mol Carcinog. 2015, 54(10):971-985. https://doi.org/10.1002/mc.22166
  13. Sulaiman MR, Perimal EK, Akhtar MN, Mohamad AS, Khalid MH, Tasrip NA, et al. Anti-inflammatory effect of zerumbone on acute and chronic inflammation models in mice. Fitoterapia. 2010, 81(7):855-858. https://doi.org/10.1016/j.fitote.2010.05.009
  14. Sulaiman MR, Perimal EK, Zakaria ZA, Mokhtar F, Akhtar MN, Lajis NH, et al. Preliminary analysis of the antinociceptive activity of zerumbone. Fitoterapia. 2009, 80(4):230-232. https://doi.org/10.1016/j.fitote.2009.02.002
  15. Sung B, Jhurani S, Ahn KS, Mastuo Y, Yi T, Guha S, et al. Zerumbone down-regulates chemokine receptor CXCR4 expression leading to inhibition of CXCL12-induced invasion of breast and pancreatic tumor cells. Cancer Res. 2008, 68(21):8938-8944 https://doi.org/10.1158/0008-5472.CAN-08-2155
  16. Taha MM, Abdul AB, Abdullah R, Ibrahim TA, Abdelwahab SI, Mohan S. Potential chemoprevention of diethylnitrosamine- initiated and 2-acetylaminofluorene-promoted hepatocarcinogenesis by zerumbone from the rhizomes of the subtropical ginger (Zingiber zerumbet). Chem Biol Interact. 2010, 186(3):305.
  17. Ticchioni M, Charvet C, Noraz N, Lamy L, Steinberg M, Bernard A, et al. Signaling through ZAP-70 is required for CXCL12-mediated T-cell transendothelial migration. Blood. 2002, 99(9):3111-3118. https://doi.org/10.1182/blood.V99.9.3111
  18. Xian M, Ito K, Nakazato T, Shimizu T, Chen CK, Yamato K, et al. Zerumbone, a bioactive sesquiterpene, induces G2/M cell cycle arrest and apoptosis in leukemia cells via a Fas- and mitochondria-mediated pathway. Cancer Sci. 2007, 98(1):118-126. https://doi.org/10.1111/j.1349-7006.2006.00362.x
  19. Zhang S, Liu Q, Liu Y, Oiao H, Liu Y. Zerumbone, a Southeast Asian ginger sesquiterpene, induced apoptosis of pancreatic carcinoma cells through p53 signaling pathway. Evid Based Complement Alternat Med. 2012, 2012:936030.

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