Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts on Blood Glucose and Lipid Metabolism in STZ-induced Diabetic Rats

돼지감자 추출액이 Streptozotocin으로 유발된 당뇨쥐에서 혈당 및 지질대사에 미치는 효과

  • Kim, Hye-Jeong (Department of Biomedical Laboratory Science, Kyungwoon University) ;
  • Kim, Dong-Il (Department of Health Care & Biotechnology, Kyungwoon University) ;
  • Yon, Jung-Min (Research Institute of Veterinary Medicine, Chungbuk National University)
  • 김혜정 (경운대학교 임상병리학과) ;
  • 김동일 (경운대학교 보건바이오학과) ;
  • 연정민 (충북대학교 동물의학연구소)
  • Received : 2015.10.22
  • Accepted : 2015.12.01
  • Published : 2015.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the effect of Jerusalem artichoke (Helianthus tuberosus L.) extract on Blood Glucose and Lipid Metabolism in Streptozotocin (70 mg/kg B.W., i.p.)-induced Diabetic Rats. Sprague-Dawley male rats (200~220 g) were divided into normal control group (NC), diabetic control group (DC) and Jerusalem artichoke treated diabetic group (DJ). Diabetes was induced by single intraperitoneal injection of streptozotocin as a dose of 70 mg/kg body weight. Food (p<0.001) and water (p<0.05) intakes were higher in diabetic groups than the normal group. Body weight gain and food efficiency ratio were significantly lower in diabetic groups than normal group (p<0.01). However, they were higher in the DJ group than in the DC group. The serum levels of AST and ALT were significantly lower in the DJ group than in the DC group (p<0.05). The serum level of HDL-C was significantly higher in the DJ group than in the DC group (p<0.001). The serum levels of Triglyceride (p<0.05), LDL-C (p<0.001), and glucose (p<0.001) were significantly lower in the DJ group than in the DC group. At 3 and 4 weeks after the experiment, blood glucose level in the DJ group was significantly lower than the DC group (p<0.05). In conclusion, these results indicated that Jerusalem artichoke can prevent or retard the development of diabetic complications via its beneficial effects for alleviating the hyperglycemia and improved lipid metabolism.

Streptozotocin (70 mg/kg B.W., i.p.)으로 유발된 당뇨쥐에서 돼지감자(Helianthus tuberosus L.) 추출액의 혈당강하 및 혈중 지질대사 개선효과를 알아보고자, SD 랫드(200~220 g) 수컷을 3군(NC, DC, DJ)으로 나누어 돼지감자 추출액을 4주간 경구투여 하였다. 본 연구 결과 음수량과 식이량은 당뇨군에서 유의한 증가를 보였으며, 체중은 유의한 감소를 보였다(p<0.05). 그러나 당뇨돼지 감자군에서 체중감소를 줄이고 식이효율을 다소 개선하는 것으로 나타났다. 혈청 중 AST 및 ALT 농도는 당뇨대조군(DC)에 비해 당뇨 돼지감자군(DJ)은 각 14.3%, 27.3% 유의한 감소를 보였다(p<0.05). 혈청 HDL-C의 농도는 DC군에 비해 DJ군은 31.8% 유의한 증가를 보였다(p<0.05). 혈청 TG, LDL-C 및 glucose 농도는 DC군에 비해 DJ군에서 각 25.4%, 56.6% 및 33.0% 유의한 감소를 나타내었다(p<0.05). 혈중 포도당 농도를 측정한 결과 당뇨 유발 후 3주 및 4주 후 DC군에 비해 DJ군은 각 31.0%, 31.6% 유의하게 감소를 보였다(p<0.05). 이로서 돼지감자는 당뇨로 인한 체중 저하를 방지하고 혈당을 강하시키며 지방대사를 개선하는 것으로 판단된다.

Keywords

References

  1. Arbeeny CA, Meysers DS, Bergquist KE, Gregg RE. Inhibition of fatty acid synthesis decrease very-low density lipoprotein secretion in the hamster. J Lipid Res. 1992,33:843-851.
  2. Bach V, Kidmose U, Bjorn GK, Edelenbos M. Effects of harvest time and variety on sensory quality and chemical composition of Jerusalem artichoke (Helianthus tuberosus) tubers. J Food Chem. 2012,133:82-89. https://doi.org/10.1016/j.foodchem.2011.12.075
  3. Baltacioglu C, Esin A. Chips production from Jerusalem artichoke (Helianthus tuberosus L). Food Nutr Sci. 2012,3:1321-1328.
  4. Bursch W, Schulte-Hermann R. Cytoprotective effect of the prostacyclin derivative iloprost against liver cell death induced by the hepatotoxins carbon tetracholoride and brombenzen. Klin Wochenschr. 1986,7:47-50.
  5. Choi PB. Effect of long-term regular exercise and drug therapy on diabetes indicators, lipid profiles, and bone mineral density in patients with type II diabetes mellitus. Korean J Phys Edu. 2011,50:513-522.
  6. Connelly K, Kelly D, Gilbert R. Clinically relevant models of diabetic cardiac complications. Circ Res. 2007,101(6):78-80. https://doi.org/10.1161/CIRCRESAHA.106.147264
  7. Dunn JS, McLetchie NGB. Experimental alloxan diabetes in the rat. The Lancet. 1943,242:384-387. https://doi.org/10.1016/S0140-6736(00)87397-3
  8. Goldberg RB. Lipid disorders in diabetes. Diabetes Care. 1981,4:561-572. https://doi.org/10.2337/diacare.4.5.561
  9. Hong JH, Park MR, Rhee SJ. Effects of YK-209 mulberry leaves on HMG-CoA reductase and lipid composition of liver on streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr. 2002,31(5):826-833. https://doi.org/10.3746/jkfn.2002.31.5.826
  10. Jekal SJ, Lee KS, Chung OB, Lee JH. Effects of electrical stimulation on wound healing and skin mast cells in streptozotocin-induced diabetic rats. Korean J Clin Lab Sci. 2008,40(2):118-128.
  11. Kim HN, Yu SY, Yoon WB, Jang SM, Jang YJ, Lee OH. Analysis of nutritional components and physicochemical properties of hot-air dried Jerusalem artichoke (Helianthus tuberosus L.) Powder. Korean J Food Sci Technol. 2014,46:73-78. https://doi.org/10.9721/KJFST.2014.46.1.73
  12. Kim JL, Bae CR, Cha YS. Helianthus tuberosus extract has antidiabetes effects in HIT-T15 cells. J Korean Soc Food Sci Nutr. 2010,39:31-35. https://doi.org/10.3746/jkfn.2010.39.1.031
  13. Kleessen B, Schwarz S, Boehm A, Fuhrmann H, Richter A, Henle T, et al. Jerusalem artichoke and chicory inulin in bakery products affect faecal microbiota of healthy volunteers. Br J Nutr. 2007,98:540-549. https://doi.org/10.1017/S0007114507730751
  14. Korean Diabetes Association, Korea Centers for Disease Control and Prevention. Diabetes fact sheet in Korea 2012. Korea. 2012, p2. Korean Diabetes Association, Korea Centers for Disease Control and Prevention, Korea.
  15. Lee JH, Hyun SH, Park KT, Ahn TH, Kim IS. Correlation of chronic hepatitis B virus infection with diabetes mellitus indicators. Korean J Clin Lab Sci. 2013,45(1):9-15.
  16. Lee JH, Jun IN. The change of tissue lipid levels and fatty acid compositions by alloxan-induced diabetes in rats. J Korean Soc Food Sci Nutr. 2004, 33:1273-1278. https://doi.org/10.3746/jkfn.2004.33.8.1273
  17. Liao Z, Chen X, Wu M. Antidiabetic effect of flavones from Cirsium japonicum DC in diabetic rats. Arch Pharm Res. 2010,33(3):353-362. https://doi.org/10.1007/s12272-010-0302-6
  18. Ministry of Health and Welfare. Korea Health Statistics 2010: Korea National Health and Nutrition Examination Surveys (KNHANES V-1). 2010. Ministry of Health and Welfare, Korea.
  19. Park CJ, Han JS. Hypoglycemic effect of Jicama (Pachyrhizus erosus) extract on streptozotocin-induced diabetic mice. Prev Nutr Food Sci. 2015,20(2):88-93. https://doi.org/10.3746/pnf.2015.20.2.88
  20. Park SY, Pak SJ, Kang SJ, Kim NY, Kim DS, Kim MJ, et al. Effects of the C3G/D3G anthocyanins-rich black soybean testa extracts on improvement of lipid profiles in STZ-induced diabetic rats. J Nutr Health. 2015,48(4):299-309. https://doi.org/10.4163/jnh.2015.48.4.299
  21. Roberfroid MB. What is beneficial for health? The concept of functional food. Food Chem Toxicol. 1999,37:1039-1041. https://doi.org/10.1016/S0278-6915(99)00080-0
  22. Shin SH, Kwon SJ, Jo HJ, Go DH, Han JJ. Extraction and analysis of inulin from Jerusalem Artichoke. Food science and industry. 2012,45:50-58.
  23. Treayway JL, Mendys P, Hoover Dj. Glycogen phosphorylase inhibitors for the treatment of type 2 diabetes mellitus. Expert Opin Investig Drugs. 2001,10:439-454. https://doi.org/10.1517/13543784.10.3.439
  24. West KM, Ahuja MS, Bennett PH. The role of circulating glucose and triglyceride concentrations and their interaction with other "risk factors" as determinants of arterial disease in nine diabetic population samples from the WHO multi-national study. Diabetes Care. 1983,6:361-369. https://doi.org/10.2337/diacare.6.4.361
  25. Yoon JA, Son YS. Effects of fruits and stems of Opuntia ficus-indica on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr. 2009,38(2):146-153. https://doi.org/10.3746/jkfn.2009.38.2.146

Cited by

  1. 다양한 암세포 주와 MSCs에 대한 Saccharin의 항증식성 평가 vol.48, pp.3, 2016, https://doi.org/10.15324/kjcls.2016.48.3.169
  2. 약선죽(藥膳粥)에 대한 인지도 및 메뉴 개발 방향 - 대전지역 성인 여성을 중심으로 - vol.23, pp.2, 2017, https://doi.org/10.14373/jkda.2017.23.2.202
  3. 명월초, 여주 및 울금을 포함하는 돼지감자 복합물의 streptozotocin 유발 당뇨쥐에서 혈당강하 및 체내 지질개선에 미치는 영향 vol.28, pp.6, 2018, https://doi.org/10.5352/jls.2018.28.6.671
  4. Effects of Jerusalem Artichoke Extract and Inulin on Blood Glucose Levels and Insulin Secretion in Streptozotocin Induced Diabetic Mice vol.22, pp.1, 2015, https://doi.org/10.4093/jkd.2021.22.1.60