Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Induction of HaCaT Cell Apoptosis by Sodium Nitroprusside

Sodium Nitroprusside로 유발한 HaCaT Cell의 Apoptosis

  • Park, Yuri (Department of Natural Medicine Resources, Semyung University) ;
  • Moon, Cheol (Department of Clinical Laboratory Science, Semyung University) ;
  • Kim, Sa-Hyun (Department of Clinical Laboratory Science, Semyung University) ;
  • Lee, Pyeongjae (Department of Natural Medicine Resources, Semyung University)
  • 박유리 (세명대학교 자연약재과학과) ;
  • 문철 (세명대학교 임상병리학과) ;
  • 김사현 (세명대학교 임상병리학과) ;
  • 이평재 (세명대학교 자연약재과학과)
  • Received : 2015.06.01
  • Accepted : 2015.06.23
  • Published : 2015.09.30
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Abstract

Nitric Oxide (NO) has been known to play important physiological and pathological roles. In this study, Sodium nitroprusside (SNP), NO donor, induced the apoptosis of HaCaT cell, human spontaneous immortal keratinocyte, which was investigated through DAPI staining and cleavage of PARP and caspase-3 protein. However, the expression level of Bip and CHOP, involved in ER stress, was not significantly changed as compared to the control cell group. Recent studies have showed that SIRT1, $NAD^+$-dependent deacetylase, is the key protein that controls cell survival and death. SNP treatment suppressed the SIRT1 gene expression, which indicated that apoptosis induced by SNP could be implicated in SIRT1 down-regulation.

산화질소(Nitric Oxide, NO)는 생리학적, 병리학적으로 주요한 역할을 하고 있는 것으로 알려져 있다. 본 실험에서는 NO donor인 sodium nitroprusside (SNP)가 HaCaT 세포에서 apoptosis를 유도한다는 것을 DAPI염색과 PARP, caspase-3 단백질 절단을 western blot으로 확인하였다. SNP는 ER stress와 관련 있는 Bip, CHOP 유전자 발현에는 영향이 없었다. 최근 NAD+ 의존 deacetylase인 sirt1이 세포의 생존 및 사멸에 매우 중요한 단백질이라는 보고가 있다. 본 실험에서 SNP는 HaCaT 세포의 sirt1 유전자 발현을 감소시켰으며 이는 apoptosis와 관련이 있을 수 있다.

Keywords

References

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