Mycobiology

The Korean Society of Mycology (한국균학회)
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Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea

  • Khalmuratova, Irina (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Kim, Hyun (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Nam, Yoon-Jong (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Oh, Yoosun (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Jeong, Min-Ji (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Choi, Hye-Rim (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • You, Young-Hyun (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Yoon, Hyeokjun (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Kim, Jong-Guk (School of Life Sciences and Biotechnology, Institute for Microorganisms, Kyungpook National University)
  • Received : 2015.09.16
  • Accepted : 2015.09.24
  • Published : 2015.12.31
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Abstract

Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

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References

  1. Traut BH. The role of coastal ecotones: a case study of the salt marsh/upland transition zone in California. J Ecol 2005; 93:279-90. https://doi.org/10.1111/j.1365-2745.2005.00969.x
  2. Kim BS, Oh HM, Kang H, Park SS, Chun J. Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol Biotechnol 2004;14:205-11.
  3. Lange M, Habekost M, Eisenhauer N, Roscher C, Bessler H, Engels C, Oelmann Y, Scheu S, Wilcke W, Schulze ED, et al. Biotic and abiotic properties mediating plant diversity effects on soil microbial communities in an experimental grassland. PLoS One 2014;9:e96182. https://doi.org/10.1371/journal.pone.0096182
  4. Selim KA, El-Beih AA, AbdEl-Rahman TM, El-Diwany AI. Biology of endophytic fungi. Curr Res Environ Appl Mycol 2012;2:31-82. https://doi.org/10.5943/cream/2/1/3
  5. Evans HC. The endophyte-enemy release hypothesis: implications for classical biological control and plant invasions. In: Proceedings of the 12th International Symposium on Biological Control of Weeds; 2007 Apr 22-27; La Grande Motte, France. Wallingford: CAB International; 2008. p. 20-5.
  6. Hermosa R, Viterbo A, Chet I, Monte E. Plant-beneficial effects of Trichoderma and of its genes. Microbiology 2012; 158(Pt 1):17-25. https://doi.org/10.1099/mic.0.052274-0
  7. Zhang J, Wang C, Guo S, Chen J, Xiao P. Studies on the plant hormones produced by 5 species of endophytic fungi isolated from medicinal plants (Orchidacea). Zhongguo Yi Xue Ke Xue Yuan Xue Bao 1999;21:460-5.
  8. Yamada A, Ogura T, Degawa Y, Ohmasa M. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 2001;42:43-50. https://doi.org/10.1007/BF02463974
  9. Vazquez MM, Cesar, S, Azcon R, Barea JM. Interactions between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl Soil Ecol 2000;15:261-72. https://doi.org/10.1016/S0929-1393(00)00075-5
  10. Colwell RK. Biodiversity: concepts, patterns, and measurement. In: Levin SA, Carpenter SR, Godfray HC, Kinzig AP, Loreau M, Losos JB, Walker B, Wilcove DS, editors. The Princeton guide to ecology. Princeton (NJ): Princeton University Press; 2009. p. 257-63.
  11. Lambshead PJ, Platt HM, Shaw KM. The detection of differences among assemblages of marine benthic species based on an assessment of dominance and diversity. J Nat Hist 1983;17:859-74. https://doi.org/10.1080/00222938300770671
  12. Hughes JB, Bohannan BJ. Section 7 update: application of ecological diversity statistics in microbial ecology. In: Kowalchuk GA, de Bruijn FJ, Head IM, Akkermans AD, van Elsas JD, editors. Molecular microbial ecology manual. Dordrecht: Springer; 2004. p. 3223-46.
  13. Whittaker RH. Evolution of species diversity in land communities. In: Hecht MK, Steere WC, Wallace B, editors. Evolutionay biology. Vol. 10. New York: Plenum; 1977. p. 1-67.
  14. Margalef DR. Information theory in ecology. Gen Syst 1958; 3:36-71.
  15. Garcia A, Rhoden SA, Rubin Filho CJ, Nakamura CV, Pamphile JA. Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L. and their localization by scanning electron microscopy. Biol Res 2012;45:139-48. https://doi.org/10.4067/S0716-97602012000200006
  16. Kim M, You YH, Yoon H, Kim H, Seo Y, Khalmuratova I, Shin JH, Lee IJ, Choo YS, Kim JG. Genetic diversity of endophytic fungal strains isolated from the roots of coastal plants in Ulleung Island for restoration of coastal ecosystem. J Life Sci 2012;22:1384-91. https://doi.org/10.5352/JLS.2012.22.10.1384
  17. Hasan HA. Gibberellin and auxin-indole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol Immunol Hung 2002;49:105-18. https://doi.org/10.1556/AMicr.49.2002.1.11
  18. Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, et al. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature 2005;437:693-8. https://doi.org/10.1038/nature04028
  19. Celloto VR, Oliveira AJ, Goncalves JE, Watanabe CS, Matioli G, Goncalves RA. Biosynthesis of indole-3-acetic acid by new Klebsiella oxytoca free and immobilized cells on inorganic matrices. ScientificWorldJournal 2012;2012:495970.
  20. Khan AR, Ullah I, Waqas M, Shahzad R, Hong SJ, Park GS, Jung BK, Lee IJ, Shin JH. Plant growth-promoting potential of endophytic fungi isolated from Solanum nigrum leaves. World J Microbiol Biotechnol 2015;31:1461-6. https://doi.org/10.1007/s11274-015-1888-0
  21. Khan SA, Hamayun M, Yoon H, Kim HY, Suh SJ, Hwang SK, Kim JM, Lee IJ, Choo YS, Yoon UH, et al. Plant growth promotion and Penicillium citrinum. BMC Microbiol 2008;8:231. https://doi.org/10.1186/1471-2180-8-231
  22. Hamayun M, Khan SA, Iqbal I, Ahmad B, Lee IJ. Isolation of a gibberellin-producing fungus (Penicillium sp. MH7) and growth promotion of Crown daisy (Chrysanthemum coronarium). J Microbiol Biotechnol 2010;20:202-7.
  23. Waqas M, Khan AL, Kamran M, Hamayun M, Kang SM, Kim YH, Lee IJ. Endophytic fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress. Molecules 2012;17:10754-73. https://doi.org/10.3390/molecules170910754
  24. Nicoletti R, De Stefano M. Penicillium restrictum as an antagonist of plant pathogenic fungi. Dyn Biochem Process Biotechnol Mol Biol 2012;6:61-9.
  25. Nath R, Sharma GD, Barooah M. Plant growth promoting endophytic fungi isolated from tea (Camellia sinensis) shrubs of Assam, India. Appl Ecol Environ Res 2015;13:877-91.
  26. Jackson MB, Armstrong W. Formation of aerenchyma and the processes of plant ventilation in relation to soil flooding and submergence. Plant Biol 1999;1:274-87. https://doi.org/10.1111/j.1438-8677.1999.tb00253.x
  27. Wirsel SG, Leibinger W, Ernst M, Mendgen K. Genetic diversity of fungi closely associated with common reed. New Phytol 2001;149:589-98.
  28. Choi WY, Rim SO, Lee JH, Lee JM, Lee IJ, Cho KJ, Rhee IK, Kwon JB, Kim JG. Isolation of gibberellins-producing fungi from the root of several Sesamum indicum plants. J Microbiol Biotechnol 2005;15:22-8.
  29. Rim SO, Lee JH, Choi WY, Hwang SK, Suh SJ, Lee IJ, Rhee IK, Kim JG. Fusarium proliferatum KGL0401 as a new gibberellin-producing fungus. J Microbiol Biotechnol 2005; 15:809-14.
  30. Kawanabe Y, Yamane H, Murayama T, Takahashi N, Nakamura, T. Identification of gibberellin $A_3$ in mycelia of Neurospora crassa. Agric Biol Chem 1983;47:1693-4.
  31. Hamayun M, Khan SA, Khan MA, Khan AL, Kang SM, Kim SK, Joo GJ, Lee IJ. Gibberellin production by pure cultures of a new strain of Aspergillus fumigatus. World J Microbiol Biotechnol 2009;25:1785-92. https://doi.org/10.1007/s11274-009-0078-3
  32. Khan SA, Hamayun M, Kim HY, Yoon HJ, Seo JC, Choo YS, Lee IJ, Kim SD, Rhee IK, Kim JG. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol Lett 2009;31:283-7. https://doi.org/10.1007/s10529-008-9862-7