Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Comparison of Genetic Variation between Pre-practice Mature Trees and Post-practice One-year Old Seedlings in Pinus densiflora Natural Regeneration Stands

소나무 천연갱신림내 성목과 치수의 유전변이 비교

  • Ahn, Ji Young (Devision of Forest Genetic Resources, National Institute of Forest Science) ;
  • Lee, Jei Wan (Devision of Forest Genetic Resources, National Institute of Forest Science) ;
  • Lee, Seok Woo (Devision of Forest Genetic Resources, National Institute of Forest Science) ;
  • Baek, Seung Hoon (Devision of Forest Genetic Resources, National Institute of Forest Science) ;
  • Lim, Hyo In (Devision of Forest Genetic Resources, National Institute of Forest Science) ;
  • Kim, Hyun Seop (Forest Practice Research Center, National Institute of Forest Science)
  • 안지영 (국립산림과학원 산림유전자원과) ;
  • 이제완 (국립산림과학원 산림유전자원과) ;
  • 이석우 (국립산림과학원 산림유전자원과) ;
  • 백승훈 (국립산림과학원 산림유전자원과) ;
  • 임효인 (국립산림과학원 산림유전자원과) ;
  • 김현섭 (국립산림과학원 산림생산기술연구소)
  • Received : 2015.05.08
  • Accepted : 2015.10.01
  • Published : 2015.12.31
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Abstract

We studied the genetic impact of natural regeneration practices, such as Single seed tree, Group seed tree, Patch clear cutting and Alternate strip clear cutting systems, by comparing the nuclear microsatellite(nSSR) variation of post-practice natural regeneration one-year old seedlings of Pinus densiflora to that of pre-practice mature trees. The levels of genetic diversity of seedlings (A=13.6, $A_e$=4.3, $H_o$=0.571, $H_e$=0.597) were similar to those of mature trees (A=13.4, $A_e$=4.3, $H_o$=0.596, $H_e$=0.598) and the differences in the level of genetic diversity between seedlings and mature trees for each of the practices were not statistically significant. The degree of genetic differentiation between seedlings and mature trees was very low ($F_{ST}$=0.002) and the pairwise $F_{ST}$ values between seedlings and mature trees for all practices were less than 0.01. Overall, the natural regeneration practices appeared to have only minor impacts on the genetic diversity and the genetic composition in the studied P. densiflora stands. For a better understanding of the genetic effects of natural regeneration practices, subsequent studies such as temporal genetic variation of seedlings formed by crossing among post-practice mature trees should be considered.

단목모수, 군상모수, 군상개벌, 대상개벌 등의 천연갱신 방법이 적용된 소나무림의 nSSR 유전변이를 조사하였다. 작업(벌채) 전 성목과 작업 후 임내에서 자연 발생한 1년생 치수들의 유전다양성을 비교한 결과 큰 차이가 없는 것으로 나타났으며(성목: A=13.4; $A_e$=4.3; $H_o$=0.596; $H_e$=0.598, 치수: A=13.6; $A_e$=4.3; $H_o$=0.571; $H_e$=0.597), 각각의 작업종에서도 통계적으로 유의한 차이를 발견할 수 없었다. 성목과 치수의 유전분화 정도는 매우 낮은 것으로 나타났으며($F_{ST}$=0.002), 각 작업종별 유전분화 정도 역시 낮은 값($$F_{ST}{\leq_-}0.01$$)을 보여 작업 전, 후 임분의 유전구조 변화는 크지 않은 것으로 추정되었다. 결과적으로 본 연구가 수행된 소나무 임분의 경우 유전다양성과 유전적 조성 변화에 미치는 천연갱신 작업종의 효과가 두드러지지 않은 것으로 나타났다. 그러나, 천연갱신 작업종이 유전변화에 미치는 영향을 보다 정확하게 평가하기 위해서는 작업 후 남겨진 개체(모수)의 교배로부터 발생한 치수들의 시계열적 유전변이와 유전구조 변화를 지속적으로 추정할 필요가 있는 것으로 판단된다.

Keywords

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