The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Inhibitory Effect of the Ethanol Extract of Rosae rugosae Flos on the Hyperpigmentation and its Action Mechanism Induced by α-MSH

매괴화(玫瑰花) 에탄올추출물이 α-MSH로 유도된 과색소 형성 억제와 작용기전 연구

  • Lee, Jin-Ho (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • In, Myung-Hee (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Suk-Hoon (Dept. of Oriental Pharmacy, College of pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Mun, Yeun-Ja (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Dept. of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Lim, Kyu-Sang (Research Center of Traditional Korean Medicine, Wonkwang University)
  • 이진호 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 인명희 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 강석훈 (원광대학교 한약연구소 약학대학 한약학과) ;
  • 문연자 (원광대학교 한의학전문대학원 BK21-plus팀) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실) ;
  • 임규상 (원광대학교 한국전통의학연구소)
  • Received : 2015.01.09
  • Accepted : 2015.02.05
  • Published : 2015.02.25
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Abstract

Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extract of Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinase activity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERK by western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced ${\alpha}$-MSH-stimulated melanin synthesis by suppressing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression of tyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression of microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein (CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated the inhibitory effect of ERR on ${\alpha}$-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppression of tyrosinase gene through CREB/MITF/ERK pathway.

Keywords

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