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Phenolic compounds of must and wine supplemented with Muscat Bailey A grape fruit stem

송이줄기 첨가에 따른 Muscat Bailey A 포도의 발효 중 발효액 및 포도주의 생리활성 물질 함량

  • Jeong, Se-Hyun (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Chang, Eun-Ha (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hur, Youn-Young (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jeong, Sung-Min (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Nam, Jong-Chul (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Koh, Sang-Wook (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, In-Myung (Fruit Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2014.08.19
  • Accepted : 2014.11.14
  • Published : 2015.02.28

Abstract

This study investigated the phenolic compounds of must and wine supplemented with different concentrations (0% (no added stems), 1%, 2%, 3%, and 5%) of fruit stems during winemaking using Muscat Bailey A (MBA) grapes. The red color, and total anthocyanin, total polyphenol, and tannin contents of the must and wine significantly (p<0.05) increased with increasing added amounts of grape fruit stems, while the volatile acid content decreased with increasing added amounts of grape fruit stems. Catechin (8.16~23.08 mg/L), gallic acid (2.32~3.28 mg/L), trans-resveratrol (1.38~3.27 mg/L), and ferulic acid (1.51~1.59 mg/L) were detected in the must and wine via HPLC. The bioactive substance contents increased with increasing added amounts of grape fruit stems, except for ferulic acid. The DPPH $IC_{50}$ activity was higher in the wine (12 mg/L) with 5% grape fruit stems than in ascorbic acid (67 mg/L). These results suggest that the fruit stems of MBA grapes can be used as functional materials for winemaking.

이전 연구에서 포도 품종별 및 부위별 생리활성 물질을 분석한 결과 MBA 품종의 송이줄기 부위에서 trans-resveratrol 함량이 다른 품종에 비해 수십배 높았다. 이를 바탕으로 포도주 제조 시 버려지는 비가식 부위인 송이줄기를 농도별로 첨가해 발효 중 발효액 및 포도주의 폴리페놀 함량을 조사하였다. 송이줄기 첨가량에 따른 발효액의 발효 중 적색도, 총안토시안 함량, 총폴리페놀 및 탄닌 함량은 발효후기로 갈수록 송이줄기 첨가에 따라 유의적으로 증가하였고, 포도주에서도 같은 경향을 보였다. 반면 포도주의 휘발산 함량은 송이줄기 첨가량이 증가할수록 감소하는 경향을 보였다. 발효액 및 포도주의 폴리페놀 성분을 분석한 결과 catechin(8.16~23.08 mg/L)함량이 가장 높았으며 gallic acid(2.32~3.28 mg/L), trans-resveratrol(1.38~3.27 mg/L)및 ferulic acid(1.51~1.59 mg/L) 순서로 함량이 높았다. MBA 포도주의 항산화활성을 DPPH $IC_{50}$로 측정한 결과 송이줄기 함량에 비례해 활성이 증가하였으며, 송이줄기 5% 첨가한 포도주(12 mg/L)는 ascorbic acid의 DPPH $IC_{50}$(67 mg/L)보다 낮은 농도에서 높은 항산화활성을 나타내었다.

Keywords

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