Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지
DOI QR코드

DOI QR Code

Genome Wide Expression Analysis of the Effect of Pinelliae Rhizoma Extract on Psychological Stress

반하(半夏)가 스트레스로 인한 생쥐의 뇌조직 유전자변화에 미치는 영향 연구

  • Jeong, Jong-Hyo (Department of Korean Medicine, Graduate School of Dong-Shin University) ;
  • Cho, Su-In (School of Korean Medicine, Pu-san National University) ;
  • Song, Young-Gil (Department of Korean Medicine, Graduate School of Dong-Shin University) ;
  • Kim, Ha-Na (Department of Korean Medicine, Graduate School of Dong-Shin University) ;
  • Kim, Kyeong-Ok (Department of Korean Medicine, Graduate School of Dong-Shin University)
  • 정종효 (동신대학교 한의과대학 한방신경정신과학교실) ;
  • 조수인 (부산대학교 한의학전문대학원) ;
  • 송영길 (동신대학교 한의과대학 한방신경정신과학교실) ;
  • 김하나 (동신대학교 한의과대학 한방신경정신과학교실) ;
  • 김경옥 (동신대학교 한의과대학 한방신경정신과학교실)
  • Received : 2015.02.27
  • Accepted : 2015.03.19
  • Published : 2015.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Pinelliae Rhizoma has traditionally been used as an anti-depressant in oriental medicine. This study is to investigate the effect of Pinelliae Rhizoma extract (PRe) on psychological stress in genome wild expression of mice. Methods: After giving physical stress to mice, PRe was orally administered with 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, their genome changes were observed by micorarray analysis method. The genome changes were analyzed by IMAGENE 4.0, TREEVIEW, FatiGo algorithems, BOND database, cytoscape program, etc. Results: 1. PRe administered group were remained at normal level; 60% of increase was shown in expressed genes by physical stress, and 65% of decrease was shown in expressed genes by psychological stress. 2. Genes with increased expression in control group that remained at a normal state in PRe administered group were involved with the gene of a cellular metabolic process on biological process, protein binding on molecular function, and cell part on cell composition. The pathway was found to be cytokin-cytokin receptor interaction. 3. Genes with decreased expression in control group that remained at a normal state in PRe administered group were involved with the gene of a cellular metabolic process on biologiacl detail and coupled ATPaes activity on molecular function. This gene related path was Ubiquintin mediated proteolysis etc. 4. Core node genes analyzed by protein interaction network were Vinculin, Cell sdivision cycle 42 homolog (S. cerevisiae) etc. They played an important role in maintaining cytoskeleton and controlling cell cycle. Conclusions: Several genes were up-regulated and down-regulated in response to psychological stress. The expression of most of the genes that were altered in response to psychological stress was restored to normal levels in PRe treated mice. When the interaction network information was analyzed, the recovery of the core node genes in PRe treated mice indicates that this final set of genes may be the effective target of PRe.

Keywords

References

  1. Koh HJ. Stress management strategies in Primary medical care. Journal of Korean Academy of Family medicine. 2000;21(2):125-36.
  2. Selye H. Forty years of stress research. Principal remaining problems and misconceptions. Can Med Assoc J. 1976;115:53-6.
  3. Stone, E. A. Stress and catecholamines. In A. J. Friedhoff (ed.), Catecholamines and Behavior. Plenum Press. New York. 1975;2:31-72.
  4. Kang KT, Lee MK. Kyeongsang National University. Relations of stress coping style to depression and anxiety. Korean psychological Association. collection of dissertations. 2005. 402-3.
  5. Kwon BH, Lee SY. collection of dissertations. Journal of Oriental Neuropsychiatry. 1994;5(1):86.
  6. Moon JJ, Ahn KS, Choi SH. Oriental Pathology. Seoul. Komunsa. 1990:23-4,78-9.
  7. Kim JW, Hwang WW. Stress in Oriental Medicine. Korean Journal of Stress Research. 1993;1(1);120-1.
  8. Hwang WW. Psychosomatic disease. Seoul. Hanglim Publisher. 1985:18-24, 36-44.
  9. Hwang WW, Kim JH. Oriental Psychiatry. Seoul. Hyundai Medical Publisher. 1987:99-109.
  10. Kim YS. Experimental study on the Anti-stress effect of Bohyulanshin-tang on the noise stress.Graduate School of Kyunghee University. Doctoral Dissertation. 1986.
  11. Lee DJ. Experimental study on the Anti-stress effect of Bohyulanshin-tang, Kami Bohyulanshin-tang. Graduate School of Kyunghee University. Doctoral Dissertation. 1987.
  12. Kang HK. Effect of Jaeumgunbitang on the gastric ulcer and plasma catecholamines contents of rats in immobilization stress. Graduate School of Kyunghee University. Doctoral Dissertation. 1992.
  13. Ji SY. Effect of Jaeumgunbitang on the gastric ulcer and plasma catecholamines contents of rats in immobilization stress. Journal of Oriental Neuropsychiatry. 1992; 3(2):49-63.
  14. Hwang CY. Effects of Sibjeondaebo-Tang Adding Cervi Pantotrichum Cornu on the Immune Responses in Mice. Graduate School of Wonkwang University. Doctoral Dissertation. 1989.
  15. Im DS. The Antistress Effects of Semen Ziziphi Spinosae Extract. Graduate School of Dongshin University. Dissertation of Master's degree. 2003.
  16. Kim EJ. Effects of Valerianae Radix Extract on Sociopsychological Stress in Mice. Graduate School of Dongshin University. Doctoral Dissertation. 2005.
  17. Choi JH. Effects of Piperis Nigri Fructus Extract on Sociopsychological Stress in Mice. Graduate School of Dongshin University. Doctoral Dissertation. 2005.
  18. Kim KH, Jeong HW, Lee DW, Kim JS, Kim YK, Cho SI. Effects of Radix Polygalae Extract on Sociopsychological Stress. Korean Journal of Herbology. 2003;18(1):99-108.
  19. Cho SI, Kim HW, Jeong YS. Effects of Radix Polygalae Extract on Sociopsychological Stress. Korean Journal of Herbology. 2004;19(4):99-108.
  20. Kim SY. Effects of Pinelliae Rhizoma Extract on Sociopsychological Stress. Graduate School of Dongshin University. Doctoral Dissertation. 2007.
  21. Won HY. Effects of Banhahubak-Tang extract on sociopsychological stress. Graduate School of Dongshin University. Doctoral Dissertation. 2007.
  22. Editing commission of Herbology. Herbology. Seoul. Younglimsa. 2004:487-8.
  23. Ogawa N, Kuwahara K. Psychophysiology of emotioncommunication of emotion. Jpn J Psychosom Med. 1996;6:352-7.
  24. Shibasaki T, Imaki T, Hotta M, Ling N, Demura H. Psychological stress increases arousal through brain corticotropin-releasing hormone without significant increase in adrenocorticotropin and catecholamine secretion. Brain Res. 1993;618:71-5. https://doi.org/10.1016/0006-8993(93)90430-U
  25. Pavliek A, Hrda S, Flegr J. Free-Tree-freeware program of construction of phylogenetic trees on the basis of distance data and bootstrap/jacknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999;45(3):97-9.
  26. Park YE, Lee DH. A Study on Socio-Psychological Stresses affected with Health Status. 1999;16(1):61-82.
  27. Seyle H. The Stress of Life. 2nd edition, NY. Mcgraw-Hill Inc. 1976.
  28. Brenner. The Stressful Price of Prosperity, Science News, 1987, March 18, 16.
  29. Luthans F, Organizational Behavior, 4th, Mograw-Hill, 1985. 130.
  30. Hoffstein S, Gennaro DE, Fox AC, Hirsch J, Streuli F, Weissmann G : Colloidal lanthanum as a marker for im-paired plasma membrane permeability in ischemic dog myocardium, Am J Pathol 79:207-18, 1975.
  31. Ruberman W, Weinblatt E, Goldberg JD, Chaudhary BS : Psychosocial influences on mortality after myocardial infarction, N Engl J med 311:552-9, 1984. https://doi.org/10.1056/NEJM198408303110902
  32. Harper LS, Williams K, Lochner A : Lanthanum proving of cell membrane permeability in the rat heart: Pathological Versus artefactual alterations, J Electr Microsc Tech 14: 357-66, 1990. https://doi.org/10.1002/jemt.1060140411
  33. Schmiedl A, Bach F, Rehrenbach H, Schnabel PA, Richter J : Cellular distribution patterns of lanthanum and morphometry of rat hearts exposed to different degrees of ischemic stress, Anat Rec 243:496-508, 1995. https://doi.org/10.1002/ar.1092430412
  34. Soldani P, Pellegrini A, Gesi M, Leni P, Cristofani R, Paparelli A : SEM/TEM investigation of rat cardiac subcellular alterations induced by changing duration of noise stress, Anat Rec 248:521-32, 1997. https://doi.org/10.1002/(SICI)1097-0185(199708)248:4<521::AID-AR4>3.0.CO;2-O
  35. Gesi M, Riva A, Soldani P, Fornai F, natale G, Lenzi P, Pellegrini A, Paparelli A : Central and peripheral benzodiazepine ligands prevent mitochondrial damage induced by noise exposure in the rat myocardium : An ultrastructural study, Anat Rec 255:334-41, 1999. https://doi.org/10.1002/(SICI)1097-0185(19990701)255:3<334::AID-AR9>3.0.CO;2-W
  36. Kim HT, Kim H, Song CH, Han EH, Lee MS. Changes of Ultrastructure and Atrial Specific Granules of Atrial Myocytes in Dehydrated and Rehydrated Rats. Journal of Korean association of Anatomists. 2001;34:567-78.
  37. Toth T : The role of catecholamines in lethal immobilization stress, Acta Physiol Hung 75:95-9, 1990.
  38. Lee MS, Song CH, Kim HT, Han EH, Lee KS, Kim WC, Shin HY. The Effects of Immobilization Stress on the Ultrastructure of Rat Atrial Myocytes. Journal of Korean association of Physical anthropologist. 2002;15:1-13. https://doi.org/10.11637/kjpa.2002.15.1.1
  39. Ganote CE, Heide RSV : Cytoskeletal lesions in anoxic myocardial injury : A conventional and high-voltage electron-microscopic and immunofluorescence study, AM J Pathol 129:327-44, 1987.
  40. Lee SG. A Study of Stress Management in Oriental Medicine. Korean Journal of Stress Research. 2002; 10(2):31-9.
  41. Hwang WW. Psychosomatic disease. Seoul. Hanglim Publisher. 1985:33-50.
  42. Ahn SW. Understanding the Conception of Stress regard in the Oriental Medicine. Collection of dissertations of Korea institute of Oriental medicine. 1997;3(1):126.
  43. Hong WS. Jeonggyo Hwangjenaehyeongyoungchu. Seoul. Oriental Medicine Institution Press. 1985:158-9, 286.
  44. Kim SH. Oriental Neuropsychiatry. Seoul. Hanglim Publisher. 1978:152-4, 258-64, 277-84.
  45. Jineon. Jinmutaeksaminbang Second volume. Taipei. Daeryeongukpung Publisher. 1978:6.
  46. Sa YG. Chinese Internal Medicine. Sichuan. Sichuan scientific-technique Publisher. 1985:141-2.
  47. Kim JW, Kim JH, Hwang WW. Stress in Oriental Medicine. Journal of Oriental Neuropsychiatry. 1993;4(1):19-26.
  48. Jineon. Jinmutaekilbyeongjeungbangron. Seoul. Iljungsa. 1992:19.
  49. Shin HD, Jang BS, Lee JS, Jeong SH, Kim SS. Effects of Pinellia Tuber Extract on the change of the weight, tissue in epididymal fat, blood and mRNA in visceral fat of obesity rats induced by high fat diet. Journal of Oriental Rehabilitation Medicine. 1999;9(1):103-28.
  50. Kim KS, Kang HS. Studies on the Effects of Seang-Banha, Kang-Banha and Chung-Banha on the Hypermesis Gravidarum. Graduate School of Daegu University. Dissertation of Master's degree. 1997.
  51. Hong JH, Seo JC, Im SC, Jeong TY, Han SW. Effect of Pinelliae Rhizoma Herbal Acupuncture on the Release of Thymus and Activation-Regulated Chemokine (TARC) in Human Bronchial Epithelial Cell. Journal of Korean Acupuncture & Moxibustion Society. 2005;22(1):155-64.
  52. Entrez Gene: VCL vinculin, http://www.ncbi.nlm.nih.gov/sites/entrez? Db=gene&Cmd=ShowDetailView&TermTo Search=7414.
  53. Entrez Gene : CDC42 cell division cycle 42 (GTP binding protein, 25kDa).