The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Cultural Characteristics of New Cultivar of Lentinula edodes, Poongnyunko

표고 신품종 풍년고의 배양 특성

  • Received : 2015.03.05
  • Accepted : 2015.03.19
  • Published : 2015.03.31
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Abstract

In this study, a new cultivar of Lentinula edodes was bred from dikaryotic KFRI 490 and monokaryotic KFRI 536 by Di-mon method. The new breed was named "Poongnyunko" and physiological characteristics was investigated. Its optimal growing temperature was $25^{\circ}C$, and it had thin density. Its colony was colorless and did not form any epithelium. On the $7^{th}$ day of mycelial growth, "Poongnyunko" was 56.6 mm, and showed higher cellulase activity compared with "Sanlim 4-ho"(52.9 mm). On the $5^{th}$ day of mycelial growth, "Poongnyunko" was 58.0 mm, and showed higher laccase activity compared with "Sanlim 4-ho" (55.6 mm). Its pH at a substrate was 6.0. Before cultivation, the pH of sawdust substrate was 4.7-5.5 went down to 3.7-3.9 after 120-day cultivation. After 60 days of cultivation, the change of $CO_2$ was the greatest. During the cultivation, $CO_2$ concentration in a growing bag was 4.67% to 3.90%.

본 연구에서는 KFRI 490의 이핵 균사와 KFRI 536의 일핵 균사를 Di-mon 교잡을 시도하여 새로운 품종을 개발하였다. 품종명을 풍년고라 명명하였고, 생리적 특성을 파악하고자 하였다. 풍년고의 최적 균사 생장 온도는 $25^{\circ}C$이었고, 균사 밀도는 성김이었으며, 균총 색깔은 무색이고 피막은 형성되지 않았다. 배양 7일째 풍년고가 56.6 mm로 산림4의 52.9 mm보다 cellulase 활성이 높았고, 배양 5일째 laccase 활성 또한 풍년고 58.0 mm로 산림4호의 55.6 mm보다 높았다. 톱밥배지의 배양 전 pH는 4.7~5.5였고, 120일 배양이 완료되고 발생 직전의 pH는 3.7~3.9로 낮아졌다. 톱밥 배지의 $CO_2$ 변화는 60일 배양에서 가장 컸으며, 봉지 안의 배지 $CO_2$ 농도는 3.90~4.67%이었다.

Keywords

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