Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Comparative Nutritional Analysis for Marker-Free Transgenic Bt rice and Non-Transgenic Counterparts

무선발 형질전환 Bt 벼 현미의 주요 영양성분 분석

  • Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lim, Myung-Ho (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Jin-Hyoung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Qin, Yang (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Soon Ki (National Academy of Agricultural Science, Rural Development Administration)
  • 우희종 (농촌진흥청 국립농업과학원) ;
  • 신공식 (농촌진흥청 국립농업과학원) ;
  • 임명호 (농촌진흥청 국립농업과학원) ;
  • 이진형 (농촌진흥청 국립농업과학원) ;
  • 친양 (농촌진흥청 국립농업과학원) ;
  • 박순기 (농촌진흥청 국립농업과학원)
  • Received : 2015.02.10
  • Accepted : 2015.03.02
  • Published : 2015.03.31
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Abstract

The selectable marker-free rice plants containing mcry1Ac insecticidal gene isolated from Bacillus thuringiensis (Bt) were generated using a non-selection approach by Agrobacterium tumefaciens-mediated transformation. The nutritional composition of two lines of transgenic rice plants (RTB5 and RTB11) was compared with that of its non-transgenic counterpart. The results showed that, except for small differences in dietary fiber and some minerals, there was no significant difference between transgenic rice and conventional counterpart variety with respect to their nutrient composition. Most of measured levels of nutrients were within the range of values reported for other commercial cultivars, showing substantial equivalency. Therefore, the insertion of transgenes did not affect the nutritional composition of transgenic RTB5 and RTB11 rice grains.

본 연구는 Bacillus thuringiensis 유래의 살충성 mCry1Ac 유전자를 무선발 형질전환 방법으로 일미 벼에 도입하여 개발된 마커프리 형질전환 Bt 벼 2계통의 일반성분 및 주요성분(무기질, 아미노산) 함량을 확인하여 모본벼 및 다른 일반품종과 함량차이를 비교 분석 함으로서 형질전환 벼의 영양성분 동등성 여부를 확인하고자 수행되었다. 영양성분 분석결과 GM 벼 현미의 일반성분 조성 중 식이섬유 함량과 일부 무기질 함량이 모본 벼인 일미와 비교하여 다소 유의적 차이가 있었지만 일반품종에서 나타나는 함량범위 안에 포함되는 수치이며, 아미노산 성분과 대부분의 일반성분 및 무기질의 함량은 전반적으로 모본과 유의적 차이가 없었다. 따라서 형질전환 벼에서 관찰된 일부 성분 차이는 Bt 유전자의 도입 효과가 아닌 재배 환경 및 토양성분의 차이에서 기인된 것으로 형질 전환에 의한 비의도적 영양성분 변화는 없는 것으로 판단된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청 국립농업과학원

References

  1. AOAC. 1990. Official methods of analysis of the Association of Official Analytical Chemists. 15th edition. Association of Official Analytical Chemists. Washington, DC.
  2. Choi H, Moon JK, Park BS, Park HW, Park SY, Kim TS, Kim DH, Ryu TH, Kweon SJ, Kim JH. 2012. Comparative nutritional analysis for genetically modified rice, iksan483 and milyang204, and nontransgenic counterparts. J. Korean Soc. Appl. Biol. Chem. 55: 19-26.
  3. Damalas CA, Eleftherohorinos IG. 2011. Pesticide exposure, safety issues, and risk assessment indicators. Int. J. Environ. Res. Pub. Health 8: 1402-1419. https://doi.org/10.3390/ijerph8051402
  4. EFSA. 2010. Scientific opinion on statistical considerations for the safety evaluation of GMOs. EFSA J. 8: 1250-1259. https://doi.org/10.2903/j.efsa.2010.1250
  5. James C. 2013. Global Status of Commercialized Biotech/GM Crops: 2013. ISAAA Brief No. 46. ISAAA. Ithaca, NY.
  6. KFDA. 2011. Korean Food Standards Codex. Korea Food & Drug Administration. Chungwon, Korea.
  7. Kim EH, Suh SC, Park BS, Shin KS, Kweon SJ, Han EJ, Park SH, Kim YS, Kim JK. 2009. Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection. Planta 23: 397-405.
  8. Lee KP, Kim DH, Kweon SJ, Baek HJ, Ryu TH. 2008. Risk assessment and variety registration of transgenic crops. J. Plant Biotechnol. 35: 13-21. https://doi.org/10.5010/JPB.2008.35.1.013
  9. Lee SY, Park SY, Shin KS, Lee JH, Lim MH, Lee SM, Oh SW, Jeong EG, Yeo Y. 2014. Analysis of key nutrients and anti-nutrients in insect-resistant transgenic rice. Korean J. Breed. Sci. 46: 400-407. https://doi.org/10.9787/KJBS.2014.46.4.400
  10. Lee YT, Kim JK, Ha SH, Cho HS, Suh SC. 2010. Analyses of nutrient composition in genetically modified ${\beta}$-carotene biofortified rice. J. Korean Soc. Food Sci. Nutr. 39: 105-109. https://doi.org/10.3746/jkfn.2010.39.1.105
  11. Oberdoerfer RB, Shillito RD, Beuckeleer MD, Mitten DH. 2005. Rice (Oryza sativa L.) containing the bar gene is compositionally equivalent to the nontransgenic counterpart. J. Agri. Food Chem. 53: 1457-1465. https://doi.org/10.1021/jf0486500
  12. OECD. 2004. Series on the safety of novel food and feed No. 10. consensus document on compositional considerations for new varieties of rice (Oryza sativa): key food and feed nutrients and anti-nutrients. OECD. Paris, France.
  13. Park SY, Lee SM, Yeo Y, Kweon SJ, Cho HS, Kim JK. 2013. Comparison of the nutritional compositions of insectresistant and glufosinate-tolerant rice and conventional rice. J. Appl. Biol. Chem. 56: 5-9. https://doi.org/10.3839/jabc.2013.002
  14. Song BH, Kim DY, Kim SK, Kim YD, Choi KS. 1988. Distribution of amino acids and fatty acids within the degermed brown rice kernel. J. Korean Agric. Chem. Soc. 31: 7-12.
  15. Woo HJ, Chung CH, Shin KS, Ji HS, Lee KJ, Suh SC, Kweon SJ, Cho YG. 2009. A survey of the genetic components introduced into approved GM crops. J. Plant Biotechnol. 36: 106-114. https://doi.org/10.5010/JPB.2009.36.2.106
  16. Woo HJ, Lee SB, Lim MH, Lee JH, Shin KS, Cho HY. 2013. Transgene structures of marker-free transgenic Bt rice plants. J. Plant Biotechnol. 40: 135-140. https://doi.org/10.5010/JPB.2013.40.3.135
  17. Woo HJ, Suh SC, Cho YG. 2011. Strategies for developing marker-free transgenic plants. Biotechnol. Bioprocess Eng. 16: 1053-1064. https://doi.org/10.1007/s12257-011-0519-3
  18. Xin L, Xiaoyun H, Yunbo L, Guoying X, Xianbin J, Kunlun H. 2008. Comparative analysis of nutritional composition between herbicide-tolerant rice with bar gene and its non-transgenic counterpart. J. Food Compos. Anal. 21: 535-539. https://doi.org/10.1016/j.jfca.2008.06.001