Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
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The Allergy Assessments of Insect-resistant Agb0101 Rice

해충저항성 Agb0101 벼 알레르기 유발 가능성 평가 연구

  • Lee, Simyung (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Park, Sooyoun (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Yeo, Yunsoo (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Cho, Hyunsuk (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Kwon, Sunjong (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Oh, Sungduk (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Oh, Sunwoo (Division of Biosafety, Department of Agricultural Biotechnology, National academy of Agricultural Science, Rural development Administration) ;
  • Kim, Jaekwang (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Park, Soon Ki (School of Applied Biosciences, Kyungpook National University)
  • 이시명 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 박수윤 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 여윤수 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 조현석 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 권순종 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 오성덕 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 오선우 (농촌진흥청 국립농업과학원 농업생명자원부 생물안전성과) ;
  • 김재광 (국립인천대학교 생명과학기술대학) ;
  • 박순기 (경북대학교 응용생명과학부)
  • Received : 2014.10.08
  • Accepted : 2015.01.19
  • Published : 2015.03.31
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Abstract

Genetically modified crops are becoming an increasingly important feature of the agricultural landscapes. Specially, insect-resistant GM crops are the most fast growing traits in the world. We developed Insect-resistant Agb0101 rice using the cry1ac1 gene in order to support the food safety assessment of insect-resistant Agb0101 rice containing modified Cry1Ac1 (mCry1Ac1) protein. The novel protein introduced into the genetically modified crops need to be evaluated for the potential allergenecity before their introduction into the food chain to address the safety concerns of consumers for commercialization. We performed assessment for allergy. The mCry1Ac1 protein does not possess the characteristics associated with food allergens, and no posttranslational change. The mCry1Ac1 protein can be rapidly degraded in gastric and intestinal fluids and heat treatment at $100^{\circ}C$. In conclusion, there is a reasonable certainty of no harm resulting from the inclusion of the mCry1Ac1 protein in human food or animal feed.

유전자 변형작물은 현재 농업에서 중요성을 인정받고 있으며, 특히 해충저항성 GM 작물은 가장 빠르게 성장하고 있는 특성이다, 농촌진흥청은 이런 해충저항성 특성을 이용한 해충 저항성 벼 Agb0101을 개발하고, 이에 대한 안전성을 평가하고자 하였다. 상업화를 목적으로 할 때, GM 작물에 새로운 단백질이 도입되면 시장에 공급되기 전에 잠재적 알레르기 평가는 매우 중요한 요소이다. 해충저항성 벼 Agb0101에 도입된 mCry1Ac1 단백질은 전체적으로 기존에 알려진 알레르기 유발원과 비슷한 특성을 보이지 않았다, 발현 후 변이로 인한 당화는 일어나지 않았으며, 인공 소화액 실험에서 5초 이내에 분해 됨을 확인 하였고, 열에 대해서도 불안정하여 $100^{\circ}C$ 환경에서 10분 이내에 분해됨을 확인 하였다. 이상의 결과로 볼 때, 해충저항성 벼 Agb0101은 식품 또는 사료로서 알레르기를 유발하지 않는 것으로 생각된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청 국립농업과학원

References

  1. 통계청. 2014. www.kostat.go.kr
  2. Altmann, F., 2007. The role of protein glycosylation in allergy. Int Arch Allergy Immunol. 2007; 142(2) : 99-115. https://doi.org/10.1159/000096114
  3. Astwood, J. D., J. N. Leach, and R. L. Fuchs. 1996. Stability of food allergens to digestion in vitro. Nature Bio/Technol. 14 : 1269-127. https://doi.org/10.1038/nbt1096-1269
  4. Bajaj, S., A. Mohanty. 2005. Recent advances in rice biotechnology towards genetically superior transgenic rice. Plant Biotechnol J 3 : 275-307. https://doi.org/10.1111/j.1467-7652.2005.00130.x
  5. Bannon, G. A., R. E. Goodman, J. N. Leach, and E. Rice. 2002. Digestive stability in the context of assessing the potential allergenicity of food proteins. Comments Toxicol. 8 : 271-285. https://doi.org/10.1080/08865140214384
  6. Bierman, C.O., A. Pearlman, G. Shapiro, and W. Busse. 1996. Allergy, Asthma, and Immunology from Infancy to Adulthood, 3rd ed. Philadelphia, PA. WB Saunders Company.
  7. Codex Alimentarius Commission. 2003. Guideline for the Conduct of Food Safety Assessment of Foods Derived from Recombinant-DNA Plants, Annex on the Assessment of Possible Allergenicity. Codex Alimentarius Commission. Rome.
  8. FAO/WHO. 2001. Evaluation of Allergenicity of Genetically Modified Foods, Report of a Joint FAO/WHO Expert Consultation on Allergenicity of Foods Derived from Biotechnology. 22-25 January 2001. Food and Agriculture Organisation of the United Nations. Rome, Italy.
  9. Fu, T. J., U. R. Abbott, and C. Hatzos. 2002. Digestbility of food allergens and nonallergenic proteins in simulated gastric fluid and simulated intestinal fluid-A comparative study. Journal of agricultural and food chemistry. 50 : 7154-7160. https://doi.org/10.1021/jf020599h
  10. Goodman, R. E., S. Vieths, D. Hill, M. Ebisawa, S. L. Taylor, and R. V. Ree. 2008. Allergenicity assessment of genetically modified cropswhat makes sense? Nat Biotechnol. 26 : 73-81. https://doi.org/10.1038/nbt1343
  11. Herouet, C., D. J. Esdaile, B. A. Mallyon, E. Debruyne, A. Schulz, T. Currier, K. Hendrickx, R. J. Klis, and D. Rouan. 2005. Safety evaluation of the phosphinothricin acetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants. Regul. Toxicol. Pharm. 41: 134-149. https://doi.org/10.1016/j.yrtph.2004.11.002
  12. James, C. 2012. The global status of commercialized biotech/GM crops: 2012. ISAAA Briefs no. 44.
  13. James, C. 2013. Global status of commercialized biotech/GM crops: 2013, ISAAA Brief no. 46.
  14. Jiang, B. H., Y. Qu, T. Hu, T. Ni, and Z. Lin. 2007. Computational analysis of the relationship between allergenicity and digestibility of allergenic proteins in simulated gastric fluid. BHC Bioinformatics. 8: 375. https://doi.org/10.1186/1471-2105-8-375
  15. Jiao, Z, X. X. Si, G. K. Li, Z. M. Zhang, and X. P. Xu. 2010. Unintended compositional changes in transgenic rice seeds (Oryza sativa L.) studied by spectral and chromatographic analysis coupled with chemometrics methods. J. Agric. Food Chem. 58 : 1746-1754. https://doi.org/10.1021/jf902676y
  16. Kim. E. H., S. S. Suh, B. S. Park, K. S. Shin, S. J. Kweon, E. J. Han, S.H. Park J.K. Kim. 2009. Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection. Planta 230(2) : 397-405 https://doi.org/10.1007/s00425-009-0955-x
  17. Kim, H. Y. and J. H. Kim. 2007.Allergenicity of genetically modified crops. Pediatric Allergy and Respiratory Disease 17 : 166-172.
  18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 27 : 680-685.
  19. Maqbool, S. B., S. Riazuddin, N. T. Loc, A. M. R. Gatehouse, J. A. Gatehouse, and P. Christou. 2001. Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. Mol Breed 7 : 85-93. https://doi.org/10.1023/A:1009644712157
  20. McClintock, J. T., C. R. Schaffer, and R. D. Sjoblad. 1995. A comparative review of the mammalian toxicity of Bacillus thuringesisbased pesticides, Pestic. Sci., 45 : 95-105. https://doi.org/10.1002/ps.2780450202
  21. Metcalfe, D. D., J. D. Astwood, R. Townsend, H. A. Sampson, S. L. Taylor, R. L., and Fuchs. 1996. Assessment of the allergenic potential of foods derived from genetically engineered crop plants. Crit. Rev. Food Sci. Nutr. 36 : S165-S186. https://doi.org/10.1080/10408399609527763
  22. Moreno-Fierros, L., N. Garciaa, R. Gutierreza, R. Lopez-Revillab, and R.I. Vazquez-Padron. 2000. Intranasal, rectal and intraperitoneal immunization with protoxin Cry1Ac from Bacillus thuringiensis induces compartmentalized serum, intestinal, vaginal and pulmonary immune responses in Balb/c mice. Microbes. Infect. 2, 885-890. https://doi.org/10.1016/S1286-4579(00)00398-1
  23. Ofori-Anti, A. O., H. Ariyarathna, L. Chen, H. L. Lee, S. N. Pramod, and R. E. Goodman. 2008. Establishing objective detection limits for the pepsin digestion assay used in the assessment of genetically modified foods. Regul Toxicol Pharmacol.52 : 94-103. https://doi.org/10.1016/j.yrtph.2008.06.006
  24. Osterballe, M. I., T. K. Hansen, G. G. Mortz, A. Host, C. Bindslev-Jensen. 2005. The prevalence of food hypersensitivity in an unselected population of children and adults. Pediatr Allergy Immunol. 16(7) : 567-73. https://doi.org/10.1111/j.1399-3038.2005.00251.x
  25. Ramesh, S., D. Nagadhara V.D. Reddy, and K.V. Rao. 2004. Production of transgenic indica rice resistant to yellow stem borer and sapsucking insects, using super-binary vectors of Agrobacterium tumefaciens. Plant Sci 166 : 1077-1085 https://doi.org/10.1016/j.plantsci.2003.12.028
  26. Taylor, S. L., S. L, Hefle, C. Bindslev-jensen, F.M. Atkins, C. Andre, and C. Bruijnzeel-koomen. 2004. A consensus protocol for the determination of the threshold doses for allergenic foods: how much is too much? Clin Exp Allergy. 34 : 689-695. https://doi.org/10.1111/j.1365-2222.2004.1886.x
  27. Tu, J., G. Zhang, K. Datta, C. Xu, Y. He, Q. Zhang, G.S. Khush, and S.K. Datta. 2000. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin. Nat Biotechnol 18 : 1101-1104 https://doi.org/10.1038/80310
  28. United State Pharmacopoeia. 1990. The National Formulary USPXXII, NF XVII, US Pharmacopoeia Convention, Inc. Easton, PA: Mack Printing Co., 1788.
  29. Vazquez-Padron, R. I., L. Moreno-Fierros, L. Neri-Bazan, A.F. Martinez-Gil, G.A. de-la-Riva1, R. Lopez-Revilla. 2000a. Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice. Braz. J. Med. Biol. Res. 33 : 147-155. https://doi.org/10.1590/S0100-879X2000000200002
  30. Vazquez-Padron, R. I., J. Gonzales-Cabrera, C. Garcia-Tovar, L. Neri-Bazan, R. Lopez-Revilla, M. Hernandez, L. Moreno-Fierro, G.A. de la Riva. 2000b. Cry1Ac protoxin from Bacillus thuringiensis sp. kurstaki HD73 binds to surface proteins in the mouse small intestine. Biochem. Biophy. Res. Co. 271 : 54-58. https://doi.org/10.1006/bbrc.2000.2584
  31. Venge, P. 2004. Monitoring the allergic inflammation. Allergy 59(1) : 26-32. https://doi.org/10.1046/j.1398-9995.2003.00386.x
  32. Xu, W., S. Cao, X. He, Y. Luo, X. Guo, Y. Yuan, and K. Huang. 2009. Safety assessment of Cry1Ab/Ac fusion protein. Food Chem Toxicol 47 : 1459-1465. https://doi.org/10.1016/j.fct.2009.03.029
  33. Yun, H. Y., K. E. Lee, S. Y. Choi, H. S. Yang, M. H. Sohn, S. I. Lee, H. K. Park, S. H. Park, S. H. Lee, W. Y. Lee, and K. E. Kim. 2005. Wild Rice, Hypoallergenic Rice, and GMO Rice-Immuologic Comparison. Pediatric Allergy and Respiratory Disease. 15 : 117-125.