Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Preparation of fermentation broth of Sparassis latifolia containing soluble β-glucan using four Lactobacillus species

수용성 β-glucan을 함유한 꽃송이버섯 발효액의 제조

  • Jo, Han-Gyo (Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Choi, Moon-Hee (Major in Cosmetic Engineering, Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Shin, Hyun-Jae (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
  • 조한교 (조선대학교 대학원 화학공학과) ;
  • 최문희 (조선대학교 대학원 화학공학과 향장공학) ;
  • 신현재 (조선대학교 대학원 화학공학과)
  • Received : 2015.03.06
  • Accepted : 2015.03.20
  • Published : 2015.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Glucan has been shown to have a significant role in the activation of the immune system, including increased activity of macrophages and so on. Sparassis latifolia (formerly S. crispa) is an edible mushroom abundant in dietary fiber and widely known to contain high levels of ${\beta}$-glucan. In the present study, fermentation broths containing soluble ${\beta}$-glucan were prepared by fermentation with mushrooms with four Lactobacillus species (L. plantarum subsp. Plantarum, L. acidophilus, L. helveticus, and L. delbrueckii subsp. Bulgaricus). After culturing four Lactobacillus spp. in MRS broth, each Lactobacillus was inoculated into MRS broth containing S. latifolia powder 5% (w/v) at $37^{\circ}C$ in an anaerobic incubator for five days. It showed that the ${\beta}$-glucan contents were different in each fermentation sample. The suitable conditions for the preparation of mushroom fermentation broths were investigated and discussed.

본 연구에서는 꽃송이버섯 건물과 powder를 각각 Yeast Extract broth와 MRS broth에서 Lactoballius 4종(L. plantarum, L. acidophilus, L. helveticus, and L. delbrueckii)과 함께 발효시켰다. 발효액의 final pH는 pH $3.9{\pm}0.02$로 유산균의 일반적인 final pH 값과 일치함을 통해 유산균의 발효를 확인하였고, 유산균에 의한 꽃송이버섯 발효의 효과를 ${\beta}$-glucan 함량을 통하여 확인하였다. Yeast Extract broth 발효액은 YSB-2LP 1.65 g/100 g으로 ${\beta}$-glucan을 거의 함유하고 있지 않았지만 MRS broth 발효액은 Yeast Extract broth 발효액의 10배에 달하는 MPB-1LP 10.84 g/100 g을 함유하고 있었다. MPB-1LP는 꽃송이버섯 powder와 L.actobacillus plantarum으로 발효한 발효액으로 pH stress로 인해 만들어진 L. plantarum의 ${\beta}$-D-glucosidase에 의해 분해된 고분자량의 수용성 ${\beta}$-glucan이라 사료된다. 꽃송이버섯을 powder 상태로 L. plantarum과 함께 발효시켰을 때 꽃송이버섯의 수용성 ${\beta}$-glucan 추출 효율을 높일 수 있으며, 본 연구의 결과는 꽃송이버섯 유산균 발효물의 면역촉진 작용 연구를 위한 기초연구자료로 활용될 수 있을 것이다.

Keywords

References

  1. Choi MH, Han HK, Lee YJ, Jo HG and Shin HJ. 2014. In vitro anti-cancer activity of hydrophobic fractions of Sparassis latifolia extract using AGS, A529, and HepG2 cell lines. J Mushrooms. 12(4): 304-310. https://doi.org/10.14480/JM.2014.12.4.304
  2. De Man JC, Rogosa D and Sharpe ME. 1960. A medium for the cultivation of lactobacilli. J Appl Bacteriol. 23(1): 130-135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
  3. Galdeano CM and Perdigon G. 2004. Role of viability of probiotics strains in their persistence in the gut and in mucosal immune stimulation. J Appl Microbiol. 97(4): 673-681. https://doi.org/10.1111/j.1365-2672.2004.02353.x
  4. Goodridge HS, Reyes CN, Becker CA, Katsumoto TR, Ma J, Wolf AJ, Bose N, Chan ASH, Magee AS, Danielson ME, Weiss A, Vasilakos JP and Underhil DM. 2011. Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'. Nature. 472(7344): 471-475. https://doi.org/10.1038/nature10071
  5. Harada T, Miura N, Adachi Y, Nakajima M, Yadomae T and Ohno N. 2002. Effect of SCG, 1,3-$\beta$-D-glucan from Sparassis crispa on the hematopoietic response in cyclophosphamide induced leukopenic mice. Biol Pharm Bull. 25(7): 931-939. https://doi.org/10.1248/bpb.25.931
  6. Hatcher GE and Lambrecht RS. 1993. Augmentation of macrophage phagocytic activity by cell-free extracts of selected lactic acid-producing bacteria. J Dairy Sci. 76(9): 2485-2492. https://doi.org/10.3168/jds.S0022-0302(93)77583-9
  7. Kim HS, Kim JY, Ryu HS, Park HG, Kim YO, Kang JS, Kim HM, Hong JT, Kim Y and Han SB. 2010. Induction of dendritic cell maturation by $\beta$-glucan isolated from Sparassis crispa. Int Immunopharmacol. 10(10): 1284-1294. https://doi.org/10.1016/j.intimp.2010.07.012
  8. Lowry VK, Farnell MB, Ferro PJ, Swaggerty CL, Bahl A and Kogut MH. 2005. Purified $\beta$-glucan as an abiotic feed additive up-regulates the innate immune response in immature chickens against Salmonella enterica serovar Enteritidis. Int J Food Microbiol. 98(3): 309-318. https://doi.org/10.1016/j.ijfoodmicro.2004.06.008
  9. Lee SY, Lee YG, Byeon SE, Han S, Choi SS, Kim AR, Lee J, Lee SJ, Hong NS and Cho JY. 2010. Mitogen activated protein kinases are prime signalling enzymes in nitric oxide production induced by soluble $\beta$-glucan from Sparassis crispa. Arch Pharm Res. 33(11): 1753-1769. https://doi.org/10.1007/s12272-010-1107-3
  10. Lim CW, Kang KK, Yoo YB, Kim BH and Bae SH. 2012. Dietary fiber and $\beta$-glucan contents of Sparassis crispa fruit fermented with Lactobacillus brevis and Monascus pilosus. J Korean Soc Food Sci Nutr. 41(12): 1740-1746. https://doi.org/10.3746/jkfn.2012.41.12.1740
  11. Nameda S, Harada T, Miura NN, Adachi Y, Yadomae T, Nakajima M and Ohno N. 2003. Enhanced cytokine synthesis of leukocytes by a $\beta$-glucan preparation, SCG, extracted from a medicinal mushroom, Sparassis crispa. Immunopharmacol Immunotoxicol. 25(3): 321-335. https://doi.org/10.1081/IPH-120024500
  12. Oh DS, Wang SJ, Kim HS, Wi AJ, Yoon SY, Park HS and Park HH. 2014. Optimal medium compostion of cauliflower mushroom (Sparassis latifolia) cultivation using douglas fir wood chip and comparison of the fruiting body. J Kor Wood Sci Technol. 42(4): 428-438. https://doi.org/10.5658/WOOD.2014.42.4.428
  13. Ohno N, Miura NN, Nakajima M and Yadomae T. 2000. Antitumor 1,3-$\beta$-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull. 23(7): 866-872. https://doi.org/10.1248/bpb.23.866
  14. Ohno N, Harada T, Masuzawa S, Miura NN, Adachi Y, Nakajima M and Yadomae T. 2002. Antitumor activity and hematopoietic response of a $\beta$-glucan extracted from an edible and medicinal mushroom Sparassis crispa Wulf.: Fr. (Aphyllophoromycetideae). Int J Med Mushrooms. 4(1).
  15. Raja B. 2011. Veratric acid, a phenolic acid attenuates blood pressure and oxidative stress in L-NAME induced hypertensive rats. Eur J Pharmacol. 671(1): 87-94. https://doi.org/10.1016/j.ejphar.2011.08.052
  16. Spano G, Rinaldi A, Ugliano M, Moio L, Beneduce L and Massa S. 2005. A $\beta$-glucosidase gene isolated from wine Lactobacillus plantarum is regulated by abiotic stresses. J Appl Microbiol. 98(4): 855-861. https://doi.org/10.1111/j.1365-2672.2004.02521.x
  17. Tallon R, Bressolliera P and Urdaci MC. 2003. Isolation and characterization of two exopolysaccharides produced by Lactobacillus plantarum EP56. Res Microbiol. 154(10): 705-712. https://doi.org/10.1016/j.resmic.2003.09.006
  18. Tsai YT, Cheng PC and Pan TM. 2012. The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits. Appl Microbiol Biotechnol. 96(4): 853-862. https://doi.org/10.1007/s00253-012-4407-3
  19. Vuyst LD and Degeest B. 1999. Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol Rev. 23(2): 153-177. https://doi.org/10.1111/j.1574-6976.1999.tb00395.x
  20. Yamamoto K, Kimura T, Sugitachi A and Matsuura N. 2009. Anti-angiogenic and anti-metastatic effects of $\beta$-1, 3-D-glucan purified from hanabiratake, Sparassis crispa. Biol Pharma Bull. 32(2): 259-263. https://doi.org/10.1248/bpb.32.259
  21. Yang Z, Li S, Zhang X, Zeng X, Li D, Zhao Y and Zhang J. 2010. Capsular and slime-polysaccharide production by Lactobacillus rhamnosus JAAS8 isolated from Chinese sauerkraut: potential application in fermented milk products. J Biosci Bioeng. 110(1): 53-57. https://doi.org/10.1016/j.jbiosc.2009.12.010

Cited by

  1. Antioxidant and Immunological Activities of Sparassis crispa Fermented with Meyerozyma guilliermondii FM vol.45, pp.10, 2016, https://doi.org/10.3746/jkfn.2016.45.10.1398