Acknowledgement
Grant : Development of Monitoring and Diagnostic method for Environmental Animal Disease
Supported by : Rural Development Administration
Toxicology and Environmental Health Sciences
- Volume 7 Issue 5
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- Pages.272-276
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- 2015
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- 2005-9752(pISSN)
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- 2233-7784(eISSN)
DOI QR Code
Different Expression Patterns of Lysosomal Proteins Exposed to Melanin in HeLa Cells
- Park, Dong Jun (Department of Bioprocess Engineering, Chonbuk National University) ;
- Sekhon, Simranjeet Singh (Department of Microbiology, Chungbuk National University) ;
- Ahn, Ji-Young (Department of Microbiology, Chungbuk National University) ;
- Yoon, Hobaek (National Institute of Animal Science, RDA) ;
- Lee, Lyon (Applied Physiology and Comparative Pharmacology Laboratory, College of Veterinary Medicine, Western University of Health Sciences) ;
- Ko, Jung Ho (Applied Physiology and Comparative Pharmacology Laboratory, College of Veterinary Medicine, Western University of Health Sciences) ;
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Kim, Yang Hoon
(Department of Microbiology, Chungbuk National University)
;
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Min, Jiho
(Department of Bioprocess Engineering, Chonbuk National University)
- Received : 2015.09.11
- Accepted : 2015.11.03
- Published : 2015.12.30
Abstract
As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two-dimensional polyacrylamide gel electrophoresis (2-DE) after exposure of HeLa cells to melanin. The results showed 12 up- and 3-down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.