Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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The distribution and antimicrobial susceptibility of pathogenic microorganisms isolated from chicken slaughtering and processing procedure

닭 도계 및 가공공정 중 유해미생물의 분포와 항생제 감수성

  • Seol, Kuk-Hwan (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki Hyun (National Institute of Animal Science, Rural Development Administration) ;
  • Jo, Su-Mi (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Young Hwa (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Hyun-Wook (National Institute of Animal Science, Rural Development Administration) ;
  • Ham, Jun-Sang (National Institute of Animal Science, Rural Development Administration)
  • 설국환 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 조수미 (농촌진흥청 국립축산과학원) ;
  • 김영화 (농촌진흥청 국립축산과학원) ;
  • 김현욱 (농촌진흥청 국립축산과학원) ;
  • 함준상 (농촌진흥청 국립축산과학원)
  • Received : 2015.02.12
  • Accepted : 2015.03.13
  • Published : 2015.03.31
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Abstract

This study was performed to analyze the distribution and antimicrobial resistance of pathogenic microorganisms isolated from the carcass and environments of chicken processing plant located in Gyeonggi province from October to November in 2010. Chicken slaughterhouse was visited 3 times and totally 40 samples were collected from chicken carcass before and after washing (n=14), chicken cuts (n=7), cooling water (n=8), brine (n=2), cutting knives (n=7) and working plate (n=2). Whole-chicken rinsing technique (for chicken carcasses) and swab technique (for working plate and knives) were used to analyze the distribution of pathogenic microorganisms. In addition, brine and chilling water from storage tanks were gathered using sterilized tubes and used as samples. The matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for whole cell fingerprinting in combination with a dedicated bioinformatic software tool was used to identify the isolated microorganisms. The pathogenic microorganisms, such as Bacillus cereus (n=8) and Staphylococcus aureus (n=9), were isolated form the chicken processing process (chicken carcasses of before and after chilling, chicken cuts, and working plate). The antimicrobial susceptibility of those isolated microorganisms was analyzed using 21 antimicrobial agents. In the case of B. cereus, it showed 100% of resistance to subclasses of penicillins and peptides, and it also resistant to cephalothin, a member of critically important antimicrobials (CIA), however there was no resistance (100% susceptible) to vancomycin and chloramphenicol. S. aureus showed 100% resistance to subclasses of peptides and some of penicillins (penicillin and oxacillin), however, it showed 100% susceptibility to cephalosporins (cefazolin and cephalothin). All of the tested pathogens showed multi drug resistance (MDR) more than 4 subclasses and one of B. cereus and S. aureus showed resistance to 9 subclasses. After the ban on using the antimicrobials in animal feed in July 2011, there would be some change in microbial distribution and antimicrobial resistance, and it still has a need to be analyzed.

Keywords

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