The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antioxidant Activity and Inhibitory Activities of Xanthine Oxidase and Tyrosinase of Yeasts from Wild Flowers in Korea

우리나라 야생화에서 분리한 효모의 항산화 활성과 Xanthine Oxidase 및 Tyrosinase 저해활성

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology Paichai University) ;
  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology Paichai University) ;
  • Kim, Na-Mi (Global Products Research Institute, Korea Ginseng Corp.) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 김나미 (한국인삼공사 글로벌제품연구소) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2015.06.09
  • Accepted : 2015.06.17
  • Published : 2015.06.30
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Abstract

We prepared supernatants and cell-free extracts of yeasts isolated from wild flowers in Korea, and their antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase were investigated. Among them, cell-free extracts of Kuraishia capsulata UL40-2 and Sporobolomyces ruberrimus 121-Z-3 showed significantly high xanthine oxidase inhibitory activity of 46.4% and 48.3%, respectively. Starmerella bombicola 80-J-1 also showed tyrosinase inhibitory activity of 36.2% in the cell-free extract. Other antioxidant activities and tyrosinase inhibitory activities were not detected or were below 20%. Maximal production of the xanthine oxidase inhibitors were observed when Kuraishia capsulate UL40-2 and Sporobolomyces ruberrimus 121-Z-3 were cultured in the yeast extract-peptone-dextrose media at $30^{\circ}C$ for 24 hour, respectively.

제주도, 울릉도, 욕지도 등 우리나라 섬들과 계족산, 오서산, 백암산 등의 산에서 서식하는 야생화들에서 분리한 207 효모 균주들을 YPD 배지에 24시간 배양하여 각각의 무세포추출물과 배양상등액을 제조한 후 이들의 항산화활성과 Xanthine oxidase 저해활성 및 tyrosinase 저해활성을 측정하였다. 섬 지역 야생화들에서 분리한 Kuraishia capsulata UL40-2와 산 지역 야생화에서 분리한 Sporobolomyces ruberrimus 121-Z-3의 무세포추출물이 각각 46.4%와 48.3%의 비교적 높은 항통풍성 xanthine oxidase 저해활성을 보였다. 또한 Starmerella bombicola 80-J-1의 무세포추출물 역시 36.2%의 미백성 tyrosinase 저해활성을 보였다. 그 밖의 항산화활성과 tyrosinase 저해활성은 모든 분리효모들에서 없거나 30% 미만으로 미약하였다. 이들 가운데 xanthine oxidase 저해활성이 우수하였던 Kuraishia capsulate UL40-2와 Sporobolomyces ruberrimus 121-Z-3의 xanthine oxidase 저해제의 최적 생산 조건을 검토한 결과 두 효모 각각을 YPD에 접종하여 $30^{\circ}C$에서 24시간 배양하여 얻은 무세포추출물이 47.2%와 49.5%의 높은 xanthine oxidase 저해활성을 보였다.

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