The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Nematicidal and Reproduction Supression Activity of Actinomyces Isolates against Pine Wood Nematode, Bursaphelenchus xylophilus

소나무재선충에 대한 방선균 균주의 살선충 및 증식억제 효과

  • Lee, Chae Min (Department of Ecological Forest Insect Pests and Diseases Division, Forest Conservation Department, Korea Forest Research Institute) ;
  • Lim, Tae Heon (Research Institute of Agri-Bio Science Samhoub, Co., Ltd.) ;
  • Lee, Sang Myeong (School of Environmental Ecology and Tourism, College of Ecology and Environment Sciences, Kyungpook National University) ;
  • Mun, Il Sung (Department of Ecological Forest Insect Pests and Diseases Division, Forest Conservation Department, Korea Forest Research Institute) ;
  • Han, Sang Sub (Department of Forest Science, Chonbuk National University) ;
  • Lee, Dong Woon (School of Environmental Ecology and Tourism, College of Ecology and Environment Sciences, Kyungpook National University)
  • 이채민 (국립산림과학원) ;
  • 임태헌 (삼호유비(주)) ;
  • 이상명 (경북대학교 생태환경대학 생태환경관광학부) ;
  • 문일성 (국립산림과학원) ;
  • 한상섭 (전북대학교 산림과학과) ;
  • 이동운 (경북대학교 생태환경대학 생태환경관광학부)
  • Received : 2015.02.26
  • Accepted : 2015.06.22
  • Published : 2015.06.30
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Abstract

Thirty-two actinomycetes isolates from Korean forest soil were screened for their nematicidal and reproduction suppression activity against pine wood nematode (PWN) which is widely spread in Korea. Culture filterates of 21 isolates showed more than 90% mortality at 2-fold concentration. Among them, AM210, SG16, YD116 and YD315 were more effective than others on reproduction of PWN. The YD116 isolate was identified as Streptomyces atratus by morphological and 16S rDNA analyses. Hydrazine hydrate, similar to hydrazidomycin which has cytotoxicity among substances from S. atratus against PWN, was tested for its nematicidal activity. Ten ppm of the hydrate showed 60.8% mortality. Additional studies are needed for practical use of the S. atratus YD116 isolate.

우리나라의 소나무림에 만연되어 있는 소나무재선충에 대한 살선충 및 증식억제 활성을 우리나라 산림토양으로부터 분리 한 방선균을 이용하여 수행하였다. 우리나라 산림토양에서 분리 된 32개의 방선균 균주들 중 21개 균주의 2배 배양여액에서 소나무재선충에 대한 보정사충율이 90% 이상을 나타내었다. 방선균들 중 AM210, SG16, YD116, YD315 균주의 병원성이 가장 높았으며 YD116 균주는 분자생물학적, 형태적 특성 조사결과 Streptomyces atratus로 동정되었다. S. atratus로부터 몇 가지 활성 물질들이 알려져 있으며 그들 중 세포독성을 가지는 hydrazidomycin과 유사한 hydrazine hydrate를 이용하여 소나무재선충에 대한 살선충 활성을 검정하였다. 10 ppm 농도의 hydrazine hydrate는 소나무재선충에 대하여 60.8%의 보정사충율을 보였다. S. atratus YD116 균주의 실용화를 위한 부가적인 연구가 필요할 것이다.

Keywords

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