Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Analysis of growth pattern, gene expression and flavonoid contents under LED light wavelength in Lettuce (Lactuca sativa L.)

상추에서 LED광질에 따른 플라보노이드 생합성 관련 유전자들의 발현 및 이차대사 산물의 성분 분석

  • Jung, Yu Jin (Department of Horticulture, Hankyong National University) ;
  • Kang, Dae Hyun (Department of Plant Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Tsevelkhoroloo, Maral (Department of Plant Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Moon, Jun Kwan (Department of Plant Life & Environmental Science, Hankyong National University) ;
  • Kang, Kwon Kyoo (Department of Horticulture, Hankyong National University)
  • 정유진 (국립한경대학교 원예학과) ;
  • 강대현 (국립한경대학교 미래융합기술대학원 식물생명공학전공) ;
  • ;
  • 문준관 (국립한경대학교 식물생명환경과학과) ;
  • 강권규 (국립한경대학교 원예학과)
  • Received : 2015.06.22
  • Accepted : 2015.06.23
  • Published : 2015.06.30
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Abstract

We analyzed the effects of various LED light treatments (red 655 nm, blue 456 nm, white and mixed light) on growth pattern, gene expression and flavonoid contents in lettuce leaf. Plants treated with mixed light (red+blue+white) showed better growth performance than those treated with single LED and fluorescent lamp (FL). Expression analysis of the eight genes involved in flavonoid biosynthesis in plants treated with LED light was examined. Results showed that red lettuce grown under mixed light showed high expression levels of LsC4H, LsF3H and LsDRF genes. Morever, the same treatment plants possessed higher content of gallic acid, chlorogenic acid and quercetin contents than those in plants exposed to single light. However, the highest total anthocyanin content was identified in plants treated with red+blue light and the lowest content was identified in plants exposed to white fluorescent lamp and single LED light condition. Thus, this study indicates that the ratio of blue to red LEDs is important for the morphology, growth, and phenolic compounds with anthocyanin properties in the two lettuce cultivars tested.

본 연구는 다양한 LED광 처리가 상추의 생장상, 플라보노이드 생합성 관련 유전자들의 발현양상 및 플라보노이드 함량에 미치는 영향을 분석하였다. 상추의 생장상 분석에서 혼합광 처리가 단일 처리구보다 잎생장이 월등히 우수하였으며, 단일광내에서 청색광이 적색광보다 생체중이 높게 나타났다. LED광질에 따른 플라보노이드 생합성 관련 8개 유전자들의 발현을 분석한 결과 LsF3H와 LsDFR 유전자에서 혼합광 처리한 적상추에서 청상추보다 높게 나타났다. 또한 혼합광 처리가 단일광 처리보다 Gallic acid, Chlorogenic acidn, Quercetin 및 안토시아닌의 함량이 가장 높게 나타났다. 이상의 결과로부터 LED 광질에 따라 상추 생장과 발육차이를 보이며, 유전자의 발현 및 기능성 물질의 축적에 중요한 요인으로 작용하며, 기능성 물질의 축적을 유도하기 위해서는 적색과 청색의 혼합처리가 유용하리라 생각된다.

Keywords

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