Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-amnesic and Neuroprotective Effects of Artemisia argyi H. (Seomae mugwort) Extracts

섬애쑥 추출물의 뇌 신경세포 보호효과에 의한 학습 및 기억능력 개선 효과

  • Ha, Gi-Jeong (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Doo Sang (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung, Tae Wan (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Chang Hyeon (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Gyeongsangnamdo Agricultural Research and Extension Service) ;
  • Kim, Nak-Ku (Gyeongsangnamdo Agricultural Research and Extension Service) ;
  • Shin, Hyun-Yul (Gyeongsangnamdo Agricultural Research and Extension Service) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University)
  • 하기정 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 이두상 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 승태완 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박창현 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 진동은 (경상남도농업기술원) ;
  • 김낙구 (경상남도농업기술원) ;
  • 신현열 (경상남도농업기술원) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원)
  • Received : 2015.02.02
  • Accepted : 2015.03.24
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The anti-amnesic effect of Artemisia argyi H against trimethyltin (TMT)-induced learning and memory impairment and its neuroprotective effect against $H_2O_2$-inducedoxidative stress were investigated. Cognitive behavior was examined by Y-maze and passive avoidance test for 4 weeks, which showed improved cognitive functions in mice treated with the extract. In vitro neuroprotective effects against $H_2O_2$-induced oxidative stress were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide and lactate dehydrogenase (LDH) assays. A. argyi H. extract showed protective effects against $H_2O_2$-induced neurotoxicity; moreover, LDH release into the medium was inhibited. Finally, high-performance liquid chromatography (HPLC) analysis showed that eupatilin and jaceosidin were the major phenolic compounds in A. argyi H. extract. These results suggest that A. argyi H. could be a good source of functional substances to prevent neurodegenerative diseases.

국내에서는 최초로 황해쑥의 변종인 섬애(Seomae)쑥이란 품종명으로 산림청 품종보호등록(산림청 품종보호 제 42호, 2013.09.27.)된 고부가가치 식품 자원의 산업 활용도를 높이기 위하여 섬애쑥(Artemisia argyi H.) 60% 에탄올 추출물의 TMT 유도성 인지 결함에 대한 학습 및 기억 능력 개선 효과를 연구하였다. 뇌 신경세포 사멸을 유도하여 뇌 기능의 억제를 유발하는 TMT에 의한 인지기능 장애에 있어서 in vivo 학습 및 기억 능력을 평가하기 위해 Y-maze 및 passive avoidance test를 실시한 결과 TMT에 의해 유도된 인지기능 장애는 섬애쑥 에탄올 추출물에 의해 일정 수준에서 회복 되는 것으로 나타났다. 이들 in vivo 실험 결과의 원인 규명을 위해 동물실험이 종료된 후 mouse의 뇌 조직을 적출하여 AChE 활성 및 뇌 신경세포의 과산화 정도로써 MDA 함량을 측정한 결과, 섬애쑥 60% 에탄올 추출물을 섭취한 mouse의 경우 AChE 활성을 억제할 수 있으며 MDA 함량 역시 저해 시키는 것으로 나타났다. 특히 퇴행성 뇌신경 질환에서 중요한 발병 원인으로 알려진 산화적 스트레스에 대한 검증을 위해 섬애쑥 60% 에탄올 추출물을 이용하여 과산화수소에 의해 유발되는 in vitro 산화적 스트레스에 대한 PC12 세포에 대한 신경세포 보호효과를 확인한 결과, 60% 에탄올 추출물을 처리한 그룹은 비교적 농도 의존적으로 $H_2O_2$로 유도된 산화적 스트레스에 대한 신경세포 보호효과가 나타남을 확인할 수 있었다. 섬애쑥 에탄올 추출물에 존재하는 페놀 화합물을 HPLC로 분석한 결과 항산화 활성이 있는 eupatilin과 jaceosidin이 함유된 것으로 분석되었다. 본 연구 결과를 종합해 볼 때, TMT에 의해 유도된 학습 및 기억능력 감소 효과에 대한 섬애쑥 60% 에탄올 추출물의 개선 효과는 eupatilin과 jaceosidin 등의 페놀 화합물에서 기인되는 항산화 효과들과 이로 인한 뇌 신경세포 보호효과 그리고 일정 수준에서의 AChE 억제 효과 등에 의한 것으로 판단된다.

Keywords

References

  1. Fridovich I. Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247: 1-11 (1986) https://doi.org/10.1016/0003-9861(86)90526-6
  2. Jeong EJ, Sung SH, Kim JW, Kim SH, Kim YC. Rhus verniciflua stokes attenuates glutamate-induced neurotoxicity in primary cultures of rat cortical cells. Nat. Prod. Sci. 14: 156-160 (2008)
  3. Parfenova H, Basuroy S, Bhattacharya S, Techranova D, Qu Y, Regan RF, Leffler CW. Glutamate induces oxidative stress and apoptosis in cerebral vascular endothelial cells: contributions of $HO-_1$ and $HO-_2$ to cytoprotection. Am. J. Physiol.-Cell. Ph. 290: C1399-C1410 (2006) https://doi.org/10.1152/ajpcell.00386.2005
  4. Papandreou MA, Dimakopoulou A, Linardaki ZI, Cordopatis P, Klimis-Zacas D, Margarity M, Lamari FN. Effect of a polyphenol-rich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity. Behav. Brain Res. 198: 352-358 (2009) https://doi.org/10.1016/j.bbr.2008.11.013
  5. Lee JS, Jeong BC, Ahn YS, Chung MN, Kim HS. Color stability according to storage period of purple sweetpotato products. Korean J. Crop Sci. 51: 204-208 (2006)
  6. Whitehouse PJ, Price DL, Struble RG, Clark AW, Coyle JT, Delon MR. Alzheimer's disease and senile dementia: Loss of neurons in the basal forebrain. Science 215: 1237-1239 (1982) https://doi.org/10.1126/science.7058341
  7. Chung YK, Heo HJ, Kim EK, Kim HK, Huh TL, Lim YH, Kim SK, Shin DH. Inhibitory effect of ursolic acid purified from Origanum majorana L. on the acetylcholinesterase. Mol. Cells 11: 137-143 (2001)
  8. Murray IVJ, Sindoni ME, Axelsen PH. Promotion of oxidative lipid membrane damage by amyloid ${\beta}$ proteins. Biochemistry-US. 44: 12606-12613 (2005) https://doi.org/10.1021/bi050926p
  9. Iciek M, KwiecieI, Wlodek L. Biological properties of garlic and garlic-derived organosulfur compounds. Environ. Mol. Mutagen. 50: 247-265 (2009) https://doi.org/10.1002/em.20474
  10. Deng YM, Xie QM, Zhang SJ, Yao HY, Zhang H. Anti-asthmatic effects of perilla seed oil in the guinea pig in vitro and in vivo. Planta Med. 73: 53-58 (2007) https://doi.org/10.1055/s-2006-957062
  11. Kim RJ, Kang MJ, Hwang CR, Jung WJ, Shin JH. Antioxidant and cancer cell growth inhibition activity of five different varieties of Artemisia cultivars in Korea. J. Life Sci. 22: 844-851 (2012) https://doi.org/10.5352/JLS.2012.22.6.844
  12. Hwang YK, Kim DC, Hwang WI, Han YB. Inhibitory effect of Artemisia princeps Pampan. Extract on growth of cancer cell lines. J. Nutr. Health 31: 799-808 (1998)
  13. Rho TH, Seo GS. Growth characteristics and chemical components in local collections of Artemisia sp. Korean J. Medicinal Crop Sci. 1: 171-177 (1993)
  14. Cho YH, Chiang MH. Essential oil composition and antibacterial activity of Artemisia capillaris, Artemisia argyi, and Artemisia princeps. Korean J. Int. Agric. 13: 313-320 (2001)
  15. Kim MJ, Choi SJ, Lim ST, Kim HK, Heo HJ, Kim EK, Jun WJ, Cho HY, Kim YJ, Shin DH. Ferulic acid supplementation prevents trimethyltin-induced cognitive deficits in mice. Biosci. Biotech. Bioch. 71: 1063-1068 (2007) https://doi.org/10.1271/bbb.60564
  16. Ellman GL, Courtney KD, Andres jr V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-90 (1961) https://doi.org/10.1016/0006-2952(61)90145-9
  17. Choi SJ, Kim MJ, Heo HJ, Kim JK, Jun WJ, Kim HK, Kim EK, Kim MO, Cho HY, Hwang HJ, Kim YJ, Shin DH. Ameliorative effect of 1,2-benzenedicarboxylic acid dinonyl ester against amyloid ${\beta}$ peptide-induced neurotoxicity. Amyloid 16: 15-24 (2009) https://doi.org/10.1080/13506120802676997
  18. Kim JK, Choi SJ, Cho HY, Hwang HJ, Kim YJ, Lim ST, Kim CJ, Kim HK, Peterson S, Shin DH. Protective effects of kaempferol (3,4',5,7-tetrahydroxyflavone) against amyloid beta peptide ($A{\beta}$)-induced neurotoxicity in ICR mice. Biosci. Biotech. Bioch. 74: 397-401 (2010) https://doi.org/10.1271/bbb.90585
  19. Heo HJ, Lee CY. Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells. J. Agr. Food Chem. 53: 1984-1989 (2005) https://doi.org/10.1021/jf048616l
  20. Heo HJ, Lee CY. Phenolic phytochemicals in cabbage inhibit amyloid ${\beta}$ protein-induced neurotoxicity. LWT-Food Sci. Technol. 39: 331-337 (2006) https://doi.org/10.1016/j.lwt.2005.02.018
  21. Zhao B. Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638 (2009) https://doi.org/10.1007/s11064-008-9900-9
  22. Wang Q, Lee AY, Choi JM, Lee DG, Kim HY, Lee SH, Cho EJ. In vitro radical scavenging effect and neuroprotective activity from oxidative stress of Petasites japonicus. Kor. J. Pharmacogn. 45: 147-153 (2014)
  23. Kim MJ, Han JM, Jin YY, Back MI, Bang MH, Chung HG, Choi MS, Lee KT, Sok DE, Jeong TS. In vitro antioxidant and antiinflammatory activities of jaceosidin from Artemisia princeps Pampanini cv. Sajabal. Arch. Pharm. Res. 31: 429-437 (2008) https://doi.org/10.1007/s12272-001-1175-8
  24. Cai M, Phan PTT, Hong JG, Kim DH, Kim JM, Park SJ, Liu X, Han JE, Park H, Choi JW, Ryu JH. The neuroprotective effect of eupatilin against ischemia/reperfusion-induced delayed neuronal damage in mice. Eur. J. Pharmacol. 689: 104-110 (2012) https://doi.org/10.1016/j.ejphar.2012.05.042

Cited by

  1. Effect of gomchwi (Ligularia fischeri) extract against high glucose- and H2O2-induced oxidative stress in PC12 cells vol.48, pp.5, 2016, https://doi.org/10.9721/KJFST.2016.48.5.508