Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Total Phenolics, Total Flavonoids, and Antioxidant Capacity in the Leaves, Bulbs, and Roots of Allium hookeri

삼채의 잎, 인경, 뿌리의 총페놀 함량, 총플라보노이드 함량 및 항산화능

  • Hwang, Jeong-Seung (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Bong Han (Graduate School of Biotechnology, Kyung Hee University) ;
  • An, Xiangxue (Skin Biotechnology Center, Kyung Hee University) ;
  • Jeong, Ha Ram (Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Young-Eun (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Inil (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University) ;
  • Kim, Dae-Ok (Graduate School of Biotechnology, Kyung Hee University)
  • 황정승 (경희대학교 생명공학원) ;
  • 이봉한 (경희대학교 생명공학원) ;
  • 안향설 (경희대학교 피부생명공학센터) ;
  • 정하람 (경희대학교 생명공학원) ;
  • 김영은 (경희대학교 생명공학원) ;
  • 이인일 (경희대학교 생명공학원) ;
  • 이형재 (단국대학교 식품공학과) ;
  • 김대옥 (경희대학교 생명공학원)
  • Received : 2014.11.12
  • Accepted : 2015.02.02
  • Published : 2015.04.30
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Abstract

To quantitatively evaluate the total phenolics, total flavonoids, and antioxidant capacity in the leaves, bulbs, and roots of fresh Allium hookeri, they were extracted using various solvents including water, aqueous methanol (20, 40, 60, and 80%; v/v), and absolute methanol. The leaves had the highest levels of total phenolics (240.4-276.6 mg gallic acid equivalents/100 g) and total flavonoids (9.7-34.1 mg catechin equivalents/100 g). The highest antioxidant capacities of 78.7- 103.4 mg vitamin C equivalents (VCE)/100 g, 24.4-59.0 mg VCE/100 g, and 1,798.8-2,169.7 mg VCE/100 g in the leaves were also observed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) assays, respectively. The total phenolics had a higher linear correlation with antioxidant capacity than the total flavonoids. In general, 60% (v/v) aqueous methanol extract had higher levels of total phenolics and flavonoids, and higher antioxidant capacity than any other solvents used. This study suggested that A. hookeri might be a good source of phenolics and antioxidants.

삼채의 잎, 인경, 뿌리 세 부위를 물, 메탄올-물 혼합용매, 무수 메탄올을 이용하여 추출물을 제조한 후, 총페놀 함량, 총플라보노이드 함량 및 항산화능을 정량 분석하였다. 삼채의 부위별 총페놀 함량은 잎이 240.4-276.6 mg GAE/100 g FW, 인경이 65.5-82.8 mg GAE/100 g FW, 뿌리가 50.0-59.4 mg GAE/100 g FW를 보였다. 삼채의 부위별 총플라보노이드 함량은 잎에서 9.7-34.1 mg CE/100 g FW, 인경에서 5.0-16.7 mg CE/100 g FW, 뿌리에서는 4.4-15.3 mg CE/100 g FW로 나타났다. ABTS법, DPPH법, ORAC법을 통한 항산화능은 잎에서 각각 78.7-103.4, 24.4-59.0, 1,798.8-2,169.7 mg VCE/100 g FW, 인경에서는 35.8-51.1, 9.3-26.3, 415.3-611.7 mg VCE/100 g FW, 뿌리에서는 42.0-55.9, 12.8-24.4, 291.7-429.4 mg VCE/100 g FW을 보였다. 삼채 잎, 인경, 뿌리 중에서 잎이 가장 높은 총페놀 함량, 총플라보노이드 함량 및 항산화능을 가졌다. 6가지 추출용매에서 60% (v/v) 메탄올-물 혼합용매에서 주로 높은 활성을 나타냈다. 항산화능은 총플라보노이드 함량보다 총페놀 함량과 더 높은 상관관계를 보였다. 본 연구의 결과는 삼채가 높은 항산화능 공급할 수 있는 원료로서의 가능성을 보여 주었다는데 의의가 있다.

Keywords

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