Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of the Hot Water Extract Mixtures from Achyranthes bidentata Blume and Panax ginseng on Osteoclast and Osteoblast Differentiation

우슬과 인삼 열수추출 혼합물의 파골세포와 조골세포 분화 효과

  • Kim, Jin Seong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Ock (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Man Seok (Clinical Trial Institute, Biosafety & Validation Center, Dankook University) ;
  • Oh, Chung Hun (Clinical Trial Institute, Biosafety & Validation Center, Dankook University) ;
  • Kim, Chul Tae (Department of Emergency Medical Service, College Medical Science, Konyang University)
  • 김진성 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방만석 (단국대학교, 종합임상시험원, 바이오안전성 & 유효성 센터) ;
  • 오충훈 (단국대학교, 종합임상시험원, 바이오안전성 & 유효성 센터) ;
  • 김철태 (건양대학교, 의과학대학 응급구조학과)
  • Received : 2015.01.07
  • Accepted : 2015.02.11
  • Published : 2015.04.28
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Abstract

Osteoporosis induces a bone mineral density loss due to imbalance of bone homeostasis that is achieved by osteoclasts (which are involved in bone resorption) and osteoblasts (which are involved in bone formation). Thus, this study was performed to evaluate the effects of hot water extract of the Achyranthes bidentata Blume (ABB) and Panax ginseng (Gin) on osteoclast and osteoblast differentiation. In this study, there was no cytotoxicity by ABB, 50 and $100{\mu}g/ml$ of Gin significantly decreased cell viability of RANKL-induced osteoclast in RAW264.7 cell (p < 0.01). But, it was $50{\mu}g/ml$ of ABB and Gin mixtures increased due to protective action of ABB. Furthermore, Gin contained groups (Gin, ABB and Gin mixtures) were inhibitory effects on osteoclast differentiation and bone resorption, and increased in osteoblast differentiation activity. Gin clearly inhibited RANKL-induced osteoclast differentiation by decreased calcitonin and TRAP (p < 0.01). Also, these extracts significantly increased calcium accumulation formation of osteoblastic differentiation reagents-induced osteoblast in MC3T3-E1 cell (p < 0.05). These results suggest that ABB and Gin mixtures may be a potential as drug for the treatment of osteoporosis.

Keywords

References

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