The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Antiobese Effects of Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan in Preadipocytes and Mice Fed High Fat Diet

지방전구세포와 고지방식이비만마우스에서 가미곽향정기산의 전탕액과 발효액의 항비만효과

  • Kim, Ju Hee (Department of Pediatrics, College of Oriental Medicine, Wonkwang University) ;
  • Park, Eun Jung (Department of Pediatrics, College of Oriental Medicine, Wonkwang University)
  • 김주희 (원광대학교 한의과대학 소아과학교실) ;
  • 박은정 (원광대학교 한의과대학 소아과학교실)
  • Received : 2015.04.29
  • Accepted : 2015.05.18
  • Published : 2015.05.31
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Abstract

Objectives This experimental study was designed to investigate the antiobese effects of Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan. Methods The cellular lipid contents were assessed by Oil-Red-O staining. The expression of $PPAR{\gamma}$ and $C/EBP{\alpha}$ were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks. Results Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan is reduced 3T3-L1 cells' differentiation and the expressions of $PPAR{\gamma}$ and $C/EBP{\alpha}$ in high concentration group. High-fat diet + Fermented GamiKwakhyangjungkisan group significantly reduced body weight gain. High-fat diet + Fermented GamiKwakhyangjungkisan group significantly increased HDL-cholesterol contents and reduced LDL-cholesterol contents. Furthermore, Fermented GamiKwakhyangjungkisan is excellent antiobese effects than Gamikwakhyangjungkisan. Conclusions These results demonstrate that Gamikwakhyangjungkisan and Fermented GamiKwakhyangjungkisan exerts antiobese effect in 3T3-L1 cells and mice fed high fat diet. Furthermore, Fermented GamiKwakhyangjungkisan is excellent antiobese effects than Gamikwakhyangjungkisan.

Keywords

Acknowledgement

Supported by : 원광대학교

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