Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant and α-glucosidase inhibitory effects of ethanolic extract of Ainsliaea acerifolia and organic solvent-soluble fractions

단풍취 추출물 및 분획물의 항산화 및 α-glucosidase 저해 활성 평가

  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Taewan (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Hyun-Seok (Department of Food Science and Biotechnology, Andong National University) ;
  • Park, Youn-Moon (Department of Food Science and Biotechnology, Andong National University) ;
  • Kim, Seong-Ho (Department of Food Science and Biotechnology, Daegu University) ;
  • Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University) ;
  • Kwak, Jae Hoon (Faculty of Biotechnology Convergence, Daegu Haany University) ;
  • Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
  • 이은우 (동의대학교 생명응용학과) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 김현석 (안동대학교 식품생명공학과) ;
  • 박윤문 (안동대학교 식품생명공학과) ;
  • 김성호 (대구대학교 식품공학과) ;
  • 임무혁 (대구대학교 식품공학과) ;
  • 곽재훈 (대구한의대학교 바이오산업융합학부) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2014.09.05
  • Accepted : 2014.12.05
  • Published : 2015.04.30
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Abstract

Among the naturally occurring antioxidants, polyphenols are widely distributed in various fruits, vegetables, wines, juices, and plant-based dietary sources and divided into several subclasses that included phenolic acid, flavonoids, stilbenes, and lignans. As part of our continuing search for bioactive food ingredients, the antioxidant and ${\alpha}$-glucosidase inhibitory activities of the aqueous ethanolic extract from the aerial parts of Ainsliaea acerifolia were investigated in vitro. The antioxidant properties were evaluated via radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effect of A. acerifolia extracts was tested via ${\alpha}$-glucosidase inhibitory assay. Furthermore, the total phenolic contents were determined using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and ${\alpha}$-glucosidase inhibitory activities. In particularly, the ${\alpha}$-glucosidase inhibitory and radical scavenging properties of the ethyl acetate (EtOAc)-soluble portion from the aerial parts of the A. acerifolia were higher than those of the other solvent-soluble portions. These results suggest that A. acerifolia could be considered a new potential source of natural antioxidants and antidiabetic ingredients. More systematic investigation of the aerial parts of A. acerifolia will be performed for the further development of anti-oxidative and antidiabetic drugs.

단풍취를 70% 에탄올로 침지 추출하여 얻어진 추출물에 대해 n-hexane, EtOAc 및n-BuOH로 순차 용매 분획하였고, 얻어진 결과물에 대하여 DPPH와 $ABTS^+$ radical 소거능 및 ${\alpha}$-glucosidase 저해활성을 평가하였다. DPPH 라디칼 소거능은 페놀성 화합물의 함량이 상대적으로 높은 EtOAc층에서 $IC_{50}$ 값이 $23.4{\pm}0.3mg/mL$으로 강한 DPPH 라디칼 소거능을 확인하였고, 단풍취 추출물에 존재하는 페놀성 화합물과 라디칼 소거능과의 연관성을 시사하였다. 또한 $ABTS^+$ 라디칼 소거능은 EtOAc층의 $IC_{50}$ 값이 $19.9{\pm}2.3mg/mL$, n-BuOH층이 $IC_{50}$ 값이 $23.4{\pm}0.3mg/mL$의 우수한 라디칼 소거활성이 확인 되었고, 강한 활성물질의 존재가 시사되었다. 또한, ${\alpha}$-glucosidase 저해활성을 측정한 결과, 강한 $ABTS^+$ 라디칼 소거능을 나타낸 EtOAc 층의 $IC_{50}$은 $103.4{\pm}1.0mg/mL$의 저해활성을 나타내었으며 이는 positive control인 acarbose에 비해 우수한 활성이었으며, 추출물 상태의 시료를 단일물질로 정제할 경우 더욱 강한 효능의 화합물이 존재할 가능성을 시사하였다. 향후 이들 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며 본 연구 결과는 보다 우수한 라디칼 소거능 및 ${\alpha}$-glucosidase 저해능을 가지는 새로운 기능성 식품소재 발굴을 위한 기초자료로 활용가능하리라 사료된다.

Keywords

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