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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Stabilization and Antifungal Activity of Isolated Symbiotic Bacteria from Entomopathogenic Nematodes

곤충병원성 선충에서 분리한 공생세균의 안정화 및 항진균활성

  • Kang, Dong-Hee (Department of Biological Sciences, College of Natural Science, Keimyung University) ;
  • Kim, Hyo-Hyun (MR innovation Co., Ltd.) ;
  • Nam, Uk-Ho (MR innovation Co., Ltd.) ;
  • Kim, Hyun-Soo (Department of Biological Sciences, College of Natural Science, Keimyung University)
  • 강동희 (계명대학교 자연과학대학 생명과학과) ;
  • 김효현 ((주)엠알이노베이션) ;
  • 남욱호 ((주)엠알이노베이션) ;
  • 김현수 (계명대학교 자연과학대학 생명과학과)
  • Received : 2015.04.22
  • Accepted : 2015.06.25
  • Published : 2015.06.27
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Abstract

In order to use the symbiotic bacteria from ethomophatogenic nematodes as a biological control agent for agriculture, the cultural condition for maintaining phase I and antifungal activity was investigated. Symbiotic bacteria (SB) 1 stain from nematodes were selected from the three strains isolated from entomopathogenic nematodes. The growth of the SB 1 strain in NB, TSB, TY and YS medium was higher than that of the SB 2 and SB 3 strain. The packed cell volume of the SB 1 strain was reduced in NB medium which showed radical pH change. Phase I of the SB 1 strain was maintained in TSB medium after being stored for 2 weeks at $4^{\circ}C$. Culture broth with the SB 1 strain in TSB medium for 6 days and 7 days showed antifungal activities against Rhizoctonia solani KACC 40142, Botrytis cinerea Pers. KACC 40854, and Botrytis cinerea Pers. KACC 41008. Culture broth with the SB 1 strain in TSB medium containing 100 mM L-proline for 5 days showed antifungal activities against Rhizoctonia solani KACC 40142, and Botrytis cinerea Pers. KACC 40854.

Keywords

References

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