Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Steroid Metabolism in the Blackfin Flounder Glyptocephalus stelleri during Oocyte Maturation

기름가자미(Glyptocephalus stelleri) 성숙기 난모세포에서의 성스테로이드 호르몬 대사물질 분석

  • Lee, Hae Won (National Fisheries Research and Development Institute) ;
  • Baek, Hea Ja (Department of Marine Biology, Pukyong National University)
  • Received : 2015.08.06
  • Accepted : 2015.08.24
  • Published : 2015.08.31
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Abstract

We studied oocyte steroidogenesis in the blackfin flounder Glyptocephalus stelleri as a region-specific species, in the East Sea of Korea during the spawning season. Maturing oocytes (0.76, 0.82, 0.88, and 0.91 mm in oocyte diameter) were incubated in vitro in the presence of [$^3H$] $17{\alpha}$-hydroxyprogesterone ($[^3H]17{\alpha}$-OHP) as a precursor. Steroid metabolites were extracted from the incubated medium and oocytes, and the extracts were separated and identified by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatographymass spectrometry (GC/MS). The major metabolites produced from $[^3H]17{\alpha}$-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [$17{\beta}$-estradiol (E2) and estrone (E1)] and progestins [$17{\alpha},20{\alpha}$-dihydroxy-4-pregen-3-one ($17{\alpha}20{\alpha}P$) and $17{\alpha}20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$)] in maturing oocytes. The metabolic rate of $17{\alpha}20{\beta}$ was elevated (29.04%) in oocytes measuring 0.88 mm (nucleus migration stage following the induction of germinal vesicle breakdown), but was very low in oocytes measuring 0.76, 0.82, and 0.91 mm (0.42, 0.67, and 2.62%, respectively). From these results, we suggest that $17{\alpha}20{\beta}P$ acts as a maturation-inducing steroid in the blackfin flounder.

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References

  1. Babin PJ, Carnevali O, Lubzens E and Schneider WJ. 2007. Molecular aspects of oocyte vitellogenesis in fish. In: Babin PJ, Cerda J, Lubzens E, Ed. The Fish Oocyte: From Basic Studies to Biotechnological Applications. Springer, Dordrecht, The Netherlands, 39-76.
  2. Baek HJ. 2001. Biosynthesis of 17${\alpha}$-hydroxy, 20${\alpha}$-dihydroprogesterone by ovaries of the spotted flounder (Verasper variegatus). J Fish Sci Tech 4, 70-74.
  3. Baek HJ. 2008. Production of C21-steroids in longchin goby, Chasmichthys dolichognathus during oocyte maturation. Dev Reprod 12,107-112.
  4. Baek HJ, Kim DG and Kim HB. 2013. In vitro steroidogenesis on oocyte development in the Starry flounder, Platichthys stellatus. Dev Reprod 17, 421-426. https://doi.org/10.12717/DR.2013.17.4.421
  5. Canario AVM and Scott AP. 1990. Effects of steroids and human chorionic gonadotropin on in vitro oocyte final maturation in two marine flatfish: the dab, Limanda limanda, and the plaice, Pleuronectes platessa. Gen Comp Endocrinol 77, 161-176. https://doi.org/10.1016/0016-6480(90)90301-2
  6. Canario AVM and Scott AP. 1991. Plasma levels of ovarian steroids, including 17${\alpha}$,20${\beta}$-dihydroxy-4-pregnen-3-one and 17${\alpha}$,20${\beta}$-trihydroxy-5pregnane in female dabs (Limanda limanda)-marine flatfish-induced to mature and ovulate with human chorionic gonadotropin. Gen Comp Endocrinol 77, 177-191.
  7. Cha HK, Kwon HC, Lee SI, Yang JH, Chang DS and Chun YY. 2008. Maturity and spawning of Korean flounder, Glytocephalus stelleri (Schmidt) in the East Sea of Korea. Korean J Ichthyol 20, 263-271.
  8. Choi Y, Kim JH and Park JY. 2002. Marine Fishes of Korea. Kyo Hak Publ., Seoul, Korea.
  9. Kagawa H. 2013. Oogenesis in teleost fish. Aqua-BioScience Monographs 6, 99-127. https://doi.org/10.5047/absm.2013.00604.0099
  10. Kim IS, Choi Y, Lee CL, Lee YJ, Kim BJ and Kim JH. 2005. Illustrated book of Korean fishes. Kyo Hak Publishing Co, Seoul, Korea.
  11. Lee HW, Lee SJ, Yang JH, Lee JB Cha HK and Kim JK. 2014. Occurrence of eggs and larvae of Blackfin flounder Glyptocephalus stelleri (Pleuronectidae, Pisces) off Wangdol-cho, East Sea.
  12. Lubzens E, Young G, Bobe J and Cerda J. 2010. Oogenesis in teleosts: how eggs are formed. Gen Comp Endocrinol 165, 367-389. https://doi.org/10.1016/j.ygcen.2009.05.022
  13. Nagahama Y, Yoshikuni M, Yamashita M and Tanaka M. 1994. Regulation of Oocyte Maturation in Fish. In: Sherwood NM & CL Hew Ed., Fish Physiology. V13. Molecular Endocrinology of Fish. Academic Press, London, 393-439.
  14. National Fisheries Research and Development Institute. 2004. Commercial Fishes of the Coastal and Offshore Waters in Korea. 2nd ed. Hangul Press Busan, Korea.
  15. Ohta K, Yamaguchi S, Yamaguchi A, Okuzawa K, Kagawa H and Matsuyama M. 2002. Biosynthsis of estradiol-17${\beta}$ in the ovarin follicles of the red seabream Pagrus major during vitellogenesis. Fish Sci 68, 680-687. https://doi.org/10.1046/j.1444-2906.2002.00477.x
  16. Patino R, Thomas P and Yoshizaki G. 2003. Ovarian follicle maturation and ovulation: and integrated perspective. Fish Physiol Biochem 28, 305-308. http://dx.doi.org/10.1023/B:FISH.0000030565.74702.0a.
  17. Yoshizaki G, Shusa M, Takeuchi T and Patino R. 2001. The induction of oocyte maturational competence by gonadotropin in Nibe, Nibeamitsukurii (Teleostei, Sciaenidae) requires activation of the protein kinase A pathway, and RNA and protein synthesis. Fish Physiol Biochem 25, 201-208. https://doi.org/10.1023/A:1022209710224
  18. Yueh WS, Thomas P and Chang CF. 2005. Identification of 17,20beta,21-trihydroxy-4-pregnen-3-one as an oocyte maturation-inducing steroid in black porgy, Acanthopagrus schlegeli. Gen Comp Endocrinol 140, 184-191. http://dx.doi.org/ 10.1016/j.ygcen.2004.10.014.